ANTIMICROBIAL ACTIVITY OF ETHANOL AND AQUEOUS EXTRACTS OF BOSWELLIA DALZIELII AGAINST ENTEROPATHOGENIC ESCHERICHIA COLI AND SHIGELLA DYSENTERIAE
Keywords:
Boswellia dalzielii, phytochemical, Escherichia coli, Shigella dysenteriae, Antimicrobial.Abstract
Ethanol and aqueous extracts of stem bark of Boswellia dalzielii was used to determine the phytochemical and antibacterial activity against enteropathogenic E. coli and S. dysenteriae. The extraction was carried out using a standard method. The phytochemical screening was carried out through standard procedures and the extracts were further tested for antimicrobial activity using agar well diffusion method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also determined. Ethanol extract has the highest percentage yield of 22.26, the phytochemical tests revealed the presence of saponins, tannins, flavonoids, terpenoid, glycosides, alkaloids, triterpenes, phenols and proteins. Antimicrobial activity shows that ethanol extract has the highest mean zone of inhibition of 17.67mm and 17. 00mm at the highest concentration (250mg/ml) against S. dysenteriae and E. coli respectively, both test organisms were resistant to all concentrations of aqueous extract except at the highest concentration. MIC of aqueous extract was 250mg/ml and MBC of 500mg/ml was shown on both the test organisms. The study shows that Boswellia dalzielii stem bark ethanol and aqueous extract contain bioactive components which are responsible for its antimicrobial activities and may have the potential for the development of plant source drugs against diarrhea causing E. coli and S. dysenteriae
References
Ayoade, J.O and Oyebande, B.I. (1978). Water Resources: Geography of Nigeria Development. Pp. 71-78.
Chorley R. J (1964).Water, Earth and Man. the history of the study of landform volume I pp. 678.
Gregory, K.J. and Walling, D.E. (1973). Drainage Basin Form and Processes: AGeomorphological Approach. London: 456 pp.
Jaiyeoba, I.A. (1995).Change in soil properties relation to different land uses in part of the Nigerian semi-arid savannah, Soil Use and Management, 11, pp. 84-89.
Ogunkoya, O.O. (2000). Discrepancies in discharge records derived using the staff Gauge, staff gauge-crest stage indicator and water level recorder in S.W. Nigeria. The Nigerian Geographical Journal, New Series, 3 and 4, pp. 169-82.
Oladipo, E.O. (1985).Characteristics of thunderstorms in Zaria, Nigeria. Weather 40, p. 316
Ologe, K.O. (1971).Gully Development in the Zaria Area, Northern Nigeria (with particular reference to the Kubanni basin). Unpublished Ph.D Thesis, University of Liverpool.
Published
How to Cite
Issue
Section
FUDMA Journal of Sciences
