PHYTOCHEMICALS AND ANTIMICROBIAL ACTIVITY OF BOSWELLIA DALZIELII STEM BARK AGAINST SOME CLINICAL ISOLATES

Authors

Keywords:

: Phytochemicals, extracts, Boswellia dalzielii, antimicrobial activity, fractions

Abstract

Plants have been used in traditional medicine to treat common human illnesses in our local communities. Crude aqueous and methanol extracts of Boswellia dalzielii stem bark were screened to investigate the phytochemicals responsible for its antimicrobial activity. The crude extracts and methanol extract fractions were used to conduct antimicrobial activity against some clinical isolates (Eschericia coli, Shigella dysenteriae and Salmonella typhi). Results of the antimicrobial activity showed that both methanol and aqueous extracts had activity against the clinical isolates with higher activity observed in the methanol extract against E. coli (16.670.96 at 500 mg/ml) and S. typhi (16.170.75 at 500 mg/ml). Bioassay of the partitioned methanol extract showed n-butanol had the best activity against the organisms and the n- butanol fractions were more active against E. coli (16.00±0.00), then S. dysenteriae (14.00±0.00) and least active against S. typhi (11.00±0.00) all at 50 mg/ml. The MIC of all the organisms for both methanol and aqueous extracts is 7.81 mg/ml but 15.63 mg/ml in methanol for S. dysenteriae and the MBC range between 7.81- 125 mg/ml in both extract for all the organisms. The results of this study therefore indicated that the plants’ stem bark contain potent phytochemicals which inhibited the growth of the tested clinical isolates which validate its continued use in traditional medicine to cure illnesses. 

Dimensions

Abdulazeez, A. T., Jabeke-Togo, B., Lawal, M. and Abubakar, M. G. (2013). Phytochemical, Antibacterial and Toxicological Studies of Aqueous Stem Bark Extract of Boswellia dalzielii.African Journal of Pharmaceutical Research and Development. 5(1), pp 61-67.

Adebajo, A. O., Adewumi, C. O. and Essien, E. E (1983): Antiinfective agents of higher plants. International Symposium of Medicinal Plants. 5th ed. University of Ife, Nigeria. Pp 152-158.

Alemika, T. E., Onawunmi, G. O. and Olugbade, T. A (2004): Isolation and characterization of incensole from Boswellia dalzielii stem bark. Journal of Pharmacy and Bioresources; 1:(1), pp. 7-11

Baha’uddeen, S., Abdulkadir, M. M., Abdulrazak, M. H. and Usman, A. (2017). Determination of Phytochemicals and Antimicrobial Activity of Aqueous Stem Bark Extract of Boswellia dalzielii against Some Common Pathogenic Microorganisms. Journal of Microbiology Research, Umar Yaradua Universuty Katsina (UJMR) Volume 2:1

Baker, F. A., Silverton, R. E. and Pallinster, C. J. (1993). Introduction to Medical Laboratory Technology: 7; Pp 284-297.

Benjamin, T. T., Richard, T. S., Emmanuel, T., Pierre, S. C. F., Senghor, S. G. N., Noel, N. J., Jabeen, A., Bhatti, A. H, Maharjan, N., NgouneLeopold, T. and Jules-Roger, K. (2019). Antimicrobial and Immunomodulatory Properties of Crude Extract andCompounds fron Boswellia dalzielii Hutch. International Journal of Biosciences. Vol. 14(2), pp161-171.

Ben-Yehoshua, S., Borowitz, C. and Hanus, L. O. (2012). Frankincense, myrrh and balm of Gilead: Ancient spices of southern Arabia and Judea. Horticultural Reviews, Vol. 39 1st Ed. Willy-Blackwell publisher. Isreal. 76 pp.

Burkill, H. M. (1985): Useful plants of West Tropical Africa. White Friars Press Ltd., United Kingdom. 1:300-301.

Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Review 12:564-582.

Esimone, C. O., Attama, A. A., Mundi, K. S., Ibekwe, N. N. and Chah, K. F. (2012): Antimicrobial Activity of Psidium guajava Linn. Stem Extracts Against Methicillin-Resistant Staphylococcus aureus. African Journal of Biotechnology, 11(89), 15556-15559.

Ethuk, E. U., Agaie, B. M., Onyeyili, P. A. and Ottah, C. U. (2006). Toxicological studies of aqueous stem bark extract of Boswellia dalzielii in albino rats. J. Pharmacol. Toxicol. 38:359-360.

Hassan, H. S., Musa, A. M., Usman, M. A. and Abdulaziz, M. (2009). Preliminary Phytochemical and Antipasmodic studies of the Stem Bark of B. dalzielii. Nigerian Journal of Pharmaceutical Science, Vol. 8:1.

Hussain, H., Al-Harrasi, A., Al-Rawahi, A. and Hussain, J. (2013). Chemistry and biology of essential oils of genus Boswellia. Hindawi Publishing Corporation. Evidence-based complementary and alternative medicine Germany. pp. 12. http://doc.doi.org/10.1155/140509.

Jamil, M., Haq, U.I., Mirza, B. and Qayyum, M. (2012). Isolation of Antibacterial Compounds from Quercus dilatata L. Through Bioassay GuidedFractionation. Annals of Clinical Microbiology and Antimicrobials; 11:11, Pp2-5.

Javaid, A., Qudsia, H. and Shoaib, A. (2016). Bioassays Guided Fractionation of Senna occidentalis for Identification of Natural Antifungal Constituents against Macrophomina phaseolina. Planta Daninha 2017: V35

Karthikeyan, S., Sivakumar, A., Anbalagan, M., Nalini, E. and Gothandam, K. M. (2013). Finger printing of alkaloids, steroids and flavonoids using HPTLC of Leucasaspetra L. whole plant methanolic extract. Journal of Pharmaceutical Science Research 5(3):67-71.

Kirby, B. (1966). Antimicrobial Sensitivity Testing by Agar Diffusion Method. African Journal of Clinical Pathology 1:44-493

Leven, M., Vanden-Berghe, D. A., Morten, I. Vilienttrick, A. and Lomweas, E. C. (1979): Screening of higher plants for biological activity. Planta Medica; 36:311-312.

Mamza, U. T., Sodipo, O.A., Abdulrahman, F. I. and Khan, I. Z. (2015a). Evaluation of phytochemical, antimicrobial and toxicity studies of ethanolic stem extract of Phyllanthus amarus Thonn and Schum (Euphorbiacea). International Journal of Science and Technology 3(6):262-268.

Mamza, U. T., Sodipo, O. A., Khan, I. Z. and Gulani, I. A. (2015b). Phytochemical, antimicrobial and toxicity studies of ethanolic leaf extract of Phyllanthus amarus Thonn and Schum (Euphorbiacea). Int. J. Green. Herb.Chem. 4(1):21-30.

Mamza, U. T., Sodipo, O. A., Abdulrahman, F. I., Khan, I. Z. (2017). Proximate and Elemental Composition of Methanolic stem Bark Extract of Boswellia dalzielii (frankincense tree: Burseracea). International Journal of Science Research 6(10):751-757.

Mamza, U. T., Sodipo, A. O., Abdulrahman, F. I. and Khan, I. Z. (2018).Phytochemical Analysis and In vitro Antimicrobial Assay of the Methanol Stem Bark Extract of Boswellia dalzielii Hutch. (Burseraceae).Chemistry Research Journal. 3(4):161-168.

Mbiantcha, M., Wembe, A. N., Dawe, A., Nana, W. Y. and Ateufack, G. (2017): Antinociceptive activities of the methanolic extract of the stem bark of Boswellia dalzielii Hutch. (Burseraceae) in rats Are NO/cGMP/ATP-sensitive-k+ channel activation dependent, Evidence-Based Complementary and Alternative Medicine, vol. 2017; http://doi.org/10.1155/2017/6374907.

Miller, A. G. and Morris, M. (1988). Plants of Dhofar: Traditional, Economic and Medicinal uses, Oman Office of the adviser for conservation of the environment. Diwan of the Royal Court Sultanate of Oman. p. 360.

Mudi, S. Y., and Dauda, U. (2013).Screening and Bioassay- Guided Isolation of Antimicrobial Components from Laggera mollis. Journal of Pure and Applied Sciences (Bajopas), 6(1): 153

Nabèrè, Q., Adama, H., Samson, G., Kiessoum, K., Patrice, Z., Roland, M. N., Moussa, C., Martin, K., Jeanne, M. F. and Germaine, O. N. (2013). Antibacterial and Phytochemical studies of three Acanthaceae species used in Burkina Faso traditional medicine. Journal of Applied Pharmaceutical Science .3 (05), pp. 049-055.

Nas, F. S. and Ali, M. (2017).Antibacterial Activity of Boswellia dalzielii Leaves Extracts against Some Pathogenic Bacterial Isolates. Journal of Advances in Microbiology (JAMB), 7(1): 1-8.

Nwinyi, F. C. L., Binda, G. A., Ajoku, S. O. and Aniagu, K. S. (2004): Evaluation of aqueous extrac of Boswellia dalzielii stem bark for antimicrobial activities and gastrointestinal effects. African Journal of Biotechnology, 3:284-8

Nwade, N. and Ibrahim, M. A. (1980): Plants used in veterinary medical practice in Nigeria. Journal of veterinary pharmacol Ther, 3:261-73.

Ogunleye, D. S. and Ibittoye, S. F. (2003). Studies of antimicrobial activity and chemical Constituents of Zimenia americana. Tropical Journal of Pharmaceutical Research 2:231-241.

Ohadoma, S. C., Nnatuanya, I., Amazu, L. U. and Okolo, C. E. (2014): Antimicrobial activity of the leaf extract of fractions of Lupinusarboreus, Journal of Medicinal Plants Research. 8(8): 386-391.

Ohadoma, S. C., Amazu, L. U., Felix, N. O and Iwuji, S. (2016): Assessment of antimicrobial activity of Ethanol extracts of Commiphora Africana and Boswellia dalzielii.

Ohemu, T. L., Agunu, A., Olotu, P. N., Ajima, U., Danfam, D.G. and Azila, J. J. (2014). Ethnobotanical survey of medicinal plants used in the traditional treatment of viral infection in Jos, Plateau state- Nigeria. Int. J. Med. Arom. Plants. 4(2):74-

Parekh, J., Karathia, N. and Chanda, S. (2006). Evaluation of antibacterial activity and phytochemical analysis of Bauhinia variegate L. bark. African Journal of Biolmed. Research 9:53-56.

Perez, C., Paul, M. and Bazerque, P. (1990). An Antibiotic Assay by the Agar-well Diffusion Method. Acta Biologiae et Medicinae Experimentalis, 15, 113-115.

Pingale, S. S., Chaskar, M. G. and Kakade, N. R. (2017). Phytochemical analysis and antimicrobial activity of Caesalpinia bonducella leaves. Journal of Natural Products from Plant Resources 7(1):9-14.

Singh, K. and Tafida, G. M. (2013). Antibacterial Activity of Moringa oleifera Leaves Extracts against Some Selected Bacteria. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6:9. Pp52-54.

Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa. 2nd Ed. Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd; Screening Plants for Bioactive Agents; pp. 134–156.

Tanaka, J. C. A., da Silva, C. C., de Oliveira, A. J. B., Nakamura, C. V. and Dias Filho, B. P. (2006): Antibacterial activity of indole alkaloids from Aspidosperma ramiflorum. Brazalian Journal of Med. Biology Res.;39(3):387-391

Trease, G.E. and Evans, W.C. (2002).Pharmacognosy. 15th Ed. London: Saunders Publishers, pp. 42–393.

Vallekobia, A., Kostalova, D. and Sochorova, R. (2001). Isoquinone Alkaloid from Ahonia aquifoliusm Stem-Bark is Active Against Malassezia Species. Folia Microbiology 46: 107-111.

Vishnoi, N.R. (1979). Advanced Practical Chemistry. Ghaziabad-India: Yikas Publication House, PVT Ltd.; 1979. pp. 447–449.

Yadav, R.N.S. and Munin, A. (2011).Phytochemical analysis of some medicinal plants. Journal of Phytology, 3(12): 10-14 ISSN: 2075-6240.

Yamac, M. and Bilgili, F. (2006): Antimicrobial activities of fruit bodies and/or mycelial culture of some mushroom isolates’. Pharmaceut. Biol. 44(9):660-667.

Published

11-04-2023

How to Cite

PHYTOCHEMICALS AND ANTIMICROBIAL ACTIVITY OF BOSWELLIA DALZIELII STEM BARK AGAINST SOME CLINICAL ISOLATES. (2023). FUDMA JOURNAL OF SCIENCES, 3(3), 587-593. https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/1606

Issue

Vol. 3 No. 3 (2019): FUDMA Journal of Sciences - Vol. 3 No. 3

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Research Articles
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FUDMA Journal of Sciences

How to Cite

PHYTOCHEMICALS AND ANTIMICROBIAL ACTIVITY OF BOSWELLIA DALZIELII STEM BARK AGAINST SOME CLINICAL ISOLATES. (2023). FUDMA JOURNAL OF SCIENCES, 3(3), 587-593. https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/1606

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