EXTRACTION, ISOLATION, CHARACTERIZATION AND PHYTOCHEMICAL SCREENING OF EUPHORBIA BALSAMIFERA LEAF EXTRACT FOR PESTICIDAL ACTIVITY AGAINST CALLOSOBRUCHUS MACULATUS
Abstract
Pest infestations of stored agricultural products cause substantial global losses, highlighting the need for ecofriendly alternatives to synthetic pesticides. This study explores the insecticidal potential of Euphorbia balsamifera leaf extracts against Callosobruchus maculatus, a major storage pest. Ethanol was used to obtain the crude extracts which were then fractionated into n-hexane, chloroform, ethyl acetate, methanol, and aqueous fractions. Phytochemical analysis confirmed the presence of bioactive compounds including alkaloids, flavonoids, saponins, and terpenoids. The bioassay was conducted at varying concentrations ranging from 0.5 to 2.5 mg/µL, with each treatment performed in triplicate, and mortality rates were recorded over 72 hours. The n-hexane fraction exhibited the highest insecticidal activity, achieving 87% mortality at the highest concentration of 2.5 mg/µL. Further analysis using FT-IR and GC-MS identified key functional groups and bioactive compounds, including azelaic acid and 1-hexyl-2-nitrocyclohexane, known for their pesticidal properties. These findings suggest that Euphorbia balsamifera leaf extract could serve as natural, biodegradable alternatives to synthetic pesticides for storage pest control, contributing to sustainable pest management in agricultural.
References
Aadil, K., et al., (2022). Plant based natural products as potential ecofriendly and safer biopesticides: A Comprehensive overview of their advantages over conventional pesticides, limitations and regulatory sapects. Micrbial Pathogenesi, 173, 105854 https://doi.org/10.1016/j.micpath.2022.105854 DOI: https://doi.org/10.1016/j.micpath.2022.105854
Ahmed, S. M. S., et al., (2020). Bioefficacy of three indigenous plant extracts against callosobruchus chinensis l. (bruchidae: coleoptera). J. Asiat. Soc. Bangladesh, Sci. 46(1): 1-12, June 2020. DOI: https://doi.org/10.3329/jasbs.v46i1.54224
Ashamo, M. O., Ileke, K. D., & Onasile, A. I. (2022). Phytochemical compositions and insecticidal efficacy of four agro-waste used as biological control of cowpea beetle, Callosobruchus maculatus (Fab.) [Coleoptera: Bruchidae]. Bulletin of the National Research Centre, 46, 108. https://doi.org/10.1186/s42269-022-00795-z DOI: https://doi.org/10.1186/s42269-022-00795-z
Augusto, L. S., et al., (2021). Plant derived pesticides as an alternative to pest management and sustainable agricultural production: prospects, applications and challenges. Academic open access publishing molecule 26(16), 4835, 2021 https://doi.org/10.3390/molecules26164835 DOI: https://doi.org/10.3390/molecules26164835
Fatope M. O. Ibrahim H.and Takeda Y. (1993): Screening of Higher Plants Reputed as Herbicides Using Brine Shrimps Lethality Assay Int. J. of Pharmacog 31(4) 250 DOI: https://doi.org/10.3109/13880209309082949
Gowthama P. U., et al., (2020). Investigations into the phytochemical profile in the seeds of Elaeocarpus variabilis fruits: A potential untapped source endemic to Western Ghats. Journal of Pharmacognosy and Phytochemistry, 9(4), 566-571. DOI: https://doi.org/10.22271/phyto.2020.v9.i4h.11762
Hassan, I.U., Garba, Y.I., & Hussain, A. (2025). Effects of agrochemicals and their impact on soil quality in part of Sudan Savannah Zone of Northwestern Nigeria. FUDMA Jounal of Sciences, 9(3). https://doi.org/10.33003/fjs-2025-0903-2693 DOI: https://doi.org/10.33003/fjs-2025-0903-2693
Kabrambam D. S., et al (2021). Main plant volatile as stored grain pest management approach: A review. Journal of Agriculture and Food Research 4, 100127 https://doi.org/10.1016/j.jafr.2021.100127 DOI: https://doi.org/10.1016/j.jafr.2021.100127
Murugesan, R., et al., (2021). Insecticidal and repellent activities of Solanum torvum (Sw.) leaf extract against stored grain Pest, Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Journal of King Saud University - Science 33(3). https://doi.org/10.1016/j.jksus.2021.101390 DOI: https://doi.org/10.1016/j.jksus.2021.101390
Oladejo A.O., et al., Efficacy of Balsam Spurge [Euphorbia balsamifera (Ait.)] as Storage Protectant Against Cowpea Bruchid (Callosobruchus maculatus). Nigerian Journal of Environmental Sciences and Technology (NIJEST). Vol 7, No. 2 July 2023, pp 232 240. https://doi.org/10.36263/nijest.2023.02.0412
Pablo, P. F., & Ricard R. (2023). Neotypification of the sweet tabaiba, Euphorbia balsamifera (Euphorbiaceae). Phytotaxa 630 (2) 2023 Magnolia Press. https://doi.org/10.11646/phytotaxa.630.2.8
Riina, R., et al., (2021). More than one sweet tabaiba: Disentangling the systematics of the succulent dendroid shrub Euphorbia balsamifera. Journal Systematics and Evolution 59: 490 503. https://doi.org/10.1111/jse.12656 DOI: https://doi.org/10.1111/jse.12656
Shafeeqa et al., (2022). Antioxidant Activity and Phenolic content of sonication and maceration assisted ethanol and acetone extracts of Cymbopogon citratus leaves. Multidisciplinary Digital Publishing Institute 9(9), 244. https://doi.org/10.3390/separations9090244 DOI: https://doi.org/10.3390/separations9090244
Sharma, A., Shukla, M., Kumar, S., & Srivastava, A. (2020). Impact of synthetic pesticides on human health and environment. Frontires in Public Health, 8,398 https://doi.org/10.3389/fpubh.2020.00498 DOI: https://doi.org/10.3389/fpubh.2020.00498
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