THE EFFECT OF MEDIUM COMPOSITION ON β-GLUCOSIDASE PRODUCTION BY TRICHODERMA VIRIDE USING COW DUNG

  • S. T. Tyohemba
  • S. Aliyu
  • N. N. Ndukwe
  • G. G. Memi
  • U. O. Edem
Keywords: : β-glucosidase, Trichoderma viride, Plackett-Burman Design, Cowdung, Medium Components.

Abstract

Different medium components were screened for their effect on β-glucosidase production.  Plackett-Burman experimental design was used and twelve experimental runs were generated using Design-Expert software to determine the main effect of glucose, sucrose, lactose, carboxymethyl cellulose, peptone, beef extract, NH4Cl, NaNO3, MnCl2 CaCl2 and K2HPOon β-glucosidase production. Out of the eleven medium components selected, six components (glucose, lactose, NaNO3, NH4Cl, MnCl2, K2HPO4) were found to contribute positively to the overall β-glucosidase production with a maximum production of 34.40±0.72 U/g. Thus, the use of Plackett-Burman experimental design in this study enabled the rapid identification of important medium components affecting the production of β-glucosidase.

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Published
2020-04-14
How to Cite
TyohembaS. T., AliyuS., NdukweN. N., MemiG. G., & EdemU. O. (2020). THE EFFECT OF MEDIUM COMPOSITION ON β-GLUCOSIDASE PRODUCTION BY TRICHODERMA VIRIDE USING COW DUNG. FUDMA JOURNAL OF SCIENCES, 4(1), 105 - 111. Retrieved from https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/25