THE EFFECT OF MEDIUM COMPOSITION ON β-GLUCOSIDASE PRODUCTION BY TRICHODERMA VIRIDE USING COW DUNG
Abstract
Different medium components were screened for their effect on β-glucosidase production. Plackett-Burman experimental design was used and twelve experimental runs were generated using Design-Expert software to determine the main effect of glucose, sucrose, lactose, carboxymethyl cellulose, peptone, beef extract, NH4Cl, NaNO3, MnCl2 CaCl2 and K2HPO4 on β-glucosidase production. Out of the eleven medium components selected, six components (glucose, lactose, NaNO3, NH4Cl, MnCl2, K2HPO4) were found to contribute positively to the overall β-glucosidase production with a maximum production of 34.40±0.72 U/g. Thus, the use of Plackett-Burman experimental design in this study enabled the rapid identification of important medium components affecting the production of β-glucosidase.
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