• S. T. Tyohemba
  • S. Aliyu
  • N. N. Ndukwe
  • G. G. Memi
  • U. O. Edem
Keywords: β-glucosidase, Purification, Cow dung, Enzyme activity Trichoderma viride


β-glucosidases have characteristics of biotechnological interest and have thus become important industrial enzymes.In this study, β-glucosidase produced by Trichoderma viride from cow dung was subjected to a three step purification process involving ammonium sulphate precipitation, gel filtration by Sephadex G-100 and ion exchange chromatography by DEAE-Sephadex A-25. The elution profile on Sephadex G-100 resulted in a single broad peak (fractions 9-21) which had a yield of 3.7% and a purification fold of 4.29 with a specific activity of 25.70 µmol/min/mg proteins while the elution profile on DEAE-Sephadex A-25 resulted in a single broad peak (fraction 8-14) which had a yield of 2.76% and a purification fold of 22.14 with a specific activity of 132.41µmol/min/mg of protein. The purified enzyme was obtained as a single band and had a molecular mass of 51.8 kDa on SDS-PAGE. This results provide support for further studies of this enzyme towards revealing its potential biotechnological applications.


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How to Cite
TyohembaS. T., AliyuS., NdukweN. N., MemiG. G., & EdemU. O. (2020). PURIFICATION OF β-GLUCOSIDASE PRODUCED FROM Trichoderma viride USING COW DUNG AS CARBON SOURCE. FUDMA JOURNAL OF SCIENCES, 4(2), 190 196. https://doi.org/10.33003/fjs-2020-0402-210