• N. N. Ndukwe
  • E. O. Agbagwa
Keywords: Apiaries, Honey, Prevalence, Bacillus, Hi-Chrome Media, Characterization


Honey is a sweet viscous liquid produced by honey bee Apis mellifera from the nectar of plants. This study was carried out to determine the occurrence and identification of Bacillus species in honey samples from apiaries, locally harvested, commercial and packaged honey samples marketed in some states of Nigeria. Twenty seven honey samples were obtained from: Abia, Enugu, and Benue and Rivers state.  Honey samples were diluted with sterile distilled water and pour plated onto Hi-Chrome Bacillus agar. The isolates were identified using chromogenic media, phenotypic and Analytical Profile Index (API) kits (AP 50CHB). Nine species of Bacillus species identified include: Bacillus badius, B. cereus, B. circulans, B. laterosporus, B. licheniformis, B. megaterium, B. mycoides, B. polymyx and B. subtilis. The Bacilli count for honey samples were in the range 3.48 -2.96 log cfu/ml (Abia), 3.44 - 3.20 log cfu/ml (Benue), 3.36 - 3.15 log cfu/ml (Enugu) and 2.91-3.40 log cfu/ml (Rivers state) respectively. The occurrences of Bacillus species were significantly higher (p<0.05) in samples from commercial traders compared to Apiaries, locally harvested and the branded products. There was no significant difference (p>0.05) between the branded product samples and those from apiaries. This study has demonstrated that Bacillus species are prevalent in different sources of honey


François, E. A., Armand, P., Cokou P. A., Victorien T. D., Christine N.,Wassiyath, M., and Lamine, B.M (2018). Physicochemical Characteristics and Microbiological Quality of Honey Produced in Benin. Journal of Food Quality. Volume (1)13.https://doi.org/10.1155/2018/1896057

Afroz, R.,Tanvir E.M.,Zheng,w., and Little,P.J (2016).Molecular Pharmacology of Honey. Journal of Clinical and Experinmental Pharmacology.6:3

Libonatti, C., Soledad, V and Marina, B (2014). Antibacterial activity of honey: A review of honey around the world, Journal of Microbiology and Antimicrobials 4; 306-317.

Buba, F., Gidado, A and Shugaba, A (2013). Physicochemical and Microbiological Properties of Honey from North East Nigeria. Biochem Anal Biochem 2: 142.

Adebayo, A. A. and Davies, B. A. (2012). Microbial examination of honey marketed in south western Nigeria. Journal of Basic and Applied Scientific Research: 2(2)1701-1705.

Tawiah, A. A., Stephen, Y. G., Francis, A., Vivian, E. B and Kofi, A (2012). Antibiotic producing microorganisms from River Wiwi, Lake Bosomtwe and the Gulf of Guinea at Doakor Sea Beach, Ghana. BMC Microbiology.

Noori, S. A., Khelod, S.,Ahmed,A and Mohammad, J. A. (2012). Antibiotic, Pesticide, and Micobial Conaminant of Honey:Human Health Hazards. The scientific world Journal.(7):930849

Agbagwa, O.E, Otokunefor T.Vand Frank-Peterside N (2011). Microbiological quality of selected Nigerian honey. Global Resource Journal of Microbiology. 2: 039-042.

Amy, K. B., Brandi, M. M., Wendy, S. D., David, F. V., and Andrew, V.G (2011). Enumeration and identification of bacterial contaminants in commercial and locally produced honey.journal of microbiology (82) 103-113

Esawy, M.A., Ahmed, E.F., Helmy, W. A., Mansour, N.M., El-Senousy,W.M and El-Safty , M..M. (2011). Production of levansucrase from novel honey Bacillus subtilis isolates capable of producing antiviral levans. Carbohydrate Polymer, 36, 823-83

Babarinde,G.O.,Babarinde,S.A.,Adegbola,D.O (2011) Effects of harvesting methods on physicochemical and microbial qualities of honey. Journal of Food Science and Technology. Volume 48 (5): 628 - 634.

Hanna Rozanska (2011). Microbiological quality of polish honey. Bull Vet Inst Pulawy 55, 443-445.

Yarza, P., Ludwig, W., Euzéby, J., Amann, R., Schleifer, K. H., Glöckner, F. O and Rosselló-Móra, R. (2010). Update of the All-Species Living Tree Project based on 16S and 23S rRNA sequence analyses. Systematic and Applied Microbiology 33 (6): 291–299.

Erem, F. C and Barçın K (2009). Identification of Bacillus species isolated from ropey breads both with classical methods and api identification kits", Akdeniz Üniversitesi,

Sabate, D. C., Carrillo, L and Audisio, M. C. (2009). Inhibition of Paenibacillus honey samples. Research Microbiology, 160, 193–199.

Bogdanov, S., Jurendic, T., Sieber, R. and Gallman, P. (2008). Honey for Nutrition and Health: a review. American Journal of the College of Nutrition 27: 677 – 689.

McIntyre L, Bernard K, Beniac D, Isaac-Renton J.L, Naseby D.C (2008). Identification of Bacillus cereus group species associated with food poisoning outbreaks in British Columbia, Canada.Appllied Environmental Microbiolocy .74:7451–7453.

Stenfors, A. L.P., Fagerlund, Aand Granum P.E (2008). From soil to gut: Bacillus cereus and its food poisoning toxins. FEMS Microbiology Rev.;32 (4):579-606.
Deemah M. Al-Dabbagh (2007) Practical bacteriology manual Demonstrator, CLS413 Department King Saud University.

Kacaniova, M., Sudzina, M., Sudzinova, J., Fikselova, M., Cubon, J and Hascík, P (2007). Microbiological and physico– chemical quality of honey collected from different Slovak habitats. Slovak Journal Animal Science. 40, (1): 38 – 43.

Lawton, E.M., Ross R.P., Hill, C and Cotter, P.D (2007) Two-peptide lantibiotics: a medical perspective. Mini Rev Med Chem 7: 1236–1247.

European Commission (2005). Scientific Committee on Veterinary Measures Relating to Public Health on Honey and Microbiological Hazards. Management of Scientific Committees;Scientific Co-Operation and Networks. 1- 15.

Iurlina, M.O. (2005) Characterization of microorganisms in Argentinean honeys from different sources. International Journal of Food Microbiology. (12)151-160.

Schoeni J.L, Wong A.C (.2005) Bacillus cereus food poisoning and its toxins.Journal of Food Prot;68(3):636-48.

Barrow, G.I. and Feltham, R.K.A (2004) (eds.) Manual for the identification of medical bacteria (3rd edn.). Cambridge: Cambridge University Press.

Rayan K.J and Ray C.G (2004).Sherris Medical Microbiology (4th ed.). McGraw Hill.ISBN 0-8385-8529-9.

Xu, D., Cote, J.C. (2003). "Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences". International Journal of Systematic and Evolutionary Microbiology53 (3): 695–704.

Bryant, Vaughn M., J (2001). "Pollen Contents of Honey".CAP Newsletter24 (1): 10–24.

Cappuccino, J.G. and Sherman, N. (2001). Microbiology:A Laboratory Manual. 6th Edition. Benjamin Cummings. CA.

Cheesbrough, M (2000). Distirict laboratory practice in tropical countries Part 2. Cambridge University Press, UKp434.

Buchanan, L.E. and Gibbons, M.E. (1999).Bergey is manual of determinative bacteriology, Baltimore, the William and Willing Sco.

Wong, H.C., Chen,Y.L.,Fan,J.Y (1988).Incidence and Characterization of Bacillus cereus Isolates contaminating dairy product. Applied Environmental Microbiology.54, 699 -702.

Sneath, P.H.A., Mair, N.S., Sharpe, M.E. and Holt, J.G., (1986). Bergey’s Manual of Systematic Bacteriology, Vol. 2 Baltimore: Williams and Wilkins ISBN 0-683-07893-3
How to Cite
NdukweN. N., & AgbagwaE. O. (2020). OCCURRENCE AND IDENTIFICATION OF Bacillus SPECIES FROM DIFFERENT SOURCES OF HONEY. FUDMA JOURNAL OF SCIENCES, 4(1), 473 - 479. Retrieved from https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/70