VARIABILITY IN NUTRITIONAL QUALITY AND EVALUATING THE EFFICACY OF SSR MARKERS IN CHARACTERIZATION OF SELECTED BAMBARA GROUNDNUT ACCESSIONS
DOI:
https://doi.org/10.33003/fjs-2026-1007-4963Keywords:
Vigna subterranea L. Verdc, Proximate Analysis, Accessions, phytochemical analysis, Yield, SSR MarkersAbstract
Variety, soil conditions, climatic change, time, and biodiversity are some of the factors contributing to the inconsistent evaluation of Bambara groundnut accessions over the years. Finding Bambara groundnut accessions with excellent nutritional contents and molecular markers for efficient genetic diversity screening was the aim of this research. Five polymorphic SSR markers and ten Bambara groundnut accessions were used in the experiment. This study aimed to identify Bambara groundnut accessions with high nutritional contents and molecular markers for effective genetic diversity screening. The experiment consisted of Ten Bambara groundnut accessions and five polymorphic SSR markers. Nine proximate and phytochemical parameters were observed among the studied accessions. Wide variability was recorded among the accessions on the total flavonoid content, total phenolic content, crude protein, crude fiber, moisture content, ether extract, nitrogen free extract and total sugar content. The total flavonoid content (TFC) varied among the accessions of Bambara groundnut ranging from 5.58 – 29.19 mgGAE/g with FUKV8 recording the highest. Total phenolic content ranges from 13.48 to 5.85 mgGAE/g, crude protein ranges from 26.74% to 15.14% with FUKV6 recording the highest. The microsatellite markers under analysis had polymorphism information content (PIC) values ranging from 0 to 89%, with an average of 52%. In genetic diversity investigations, CEDG294 and PR-32 had the greatest PIC values, indicating efficiency. It is possible to classify FUKV6, FUKV18, FUKV19, and FUK10 as high-nutrient types, FUKV1 and FUKV2 as low-nutrient types, and the remaining ones as medium-nutrient types
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