EFFECTS OF EXTRACELLULAR VESICLES ISOLATED FROM SUBVENTRICULAR ZONE ON MOLECULAR CHANGES FOLLOWING SPINAL CORD INJURY ON WISTAR RATS
DOI:
https://doi.org/10.33003/fjs-2022-0601-889Keywords:
Trauma, exosome, inflammasome, NeuroinflammatoryAbstract
Spinal cord injury (SCI) may occur as a result of traumatic crash to the spine that dislocates one or many vertebrae. SCI has two events to bring about the injury in the spinal cord that result at the end it will lead to bad consequences and loss of neurons; SCI complications are general loss of motor and sensory function. Extracellular vesicles (EVs) are very small vesicles produced by cells that contribute to cell to cell communication, transferring bioactive lipids, proteins, RNA. The aim of this research is to determine the effect of EVs on inflammasomes complex after SCI on rats. EVs were harvested characterized and cultured as exosomes, the animals are divided to 5 groups: control, sham, vehicle, SCI + treatment, SCI + EVs group. Spinal cord injury was induced using weight compression. 10 μl of EVs were injected intrathecally into lumber cistern in a space between L4-L5 laminae slowly. Gene expression of Caspase-1, ASC and NLRP3 were determined by RT-PCR. Western blot for determining the protein content of the inflammasomes were carried out. SCI up regulated the level of inflammasome complex both mRNA and proteins. Inthrathecal injection of EVs down regulated the inflammasome complex and make neuronal recovery. SCI can up regulate the level of inflammasome complex both mRNA and proteins. EVs are good therapeutic agent for SCI and increase neuronal recovery, inthrathecal injection is the best method of injection MSCs for treatment of SCI.
References
Aditi, G., Kawatra, A. and Sehgal, S. (2011). Physical-Chemical Properties and Nutritional Evaluation of Newly Developed Tomato Genotypes. African Journal of Food Science and Technology. Vol: 2 (7) pp 167-172.
Aminu, Y.U and Sadi, M. (2020). Analysis of Profitability of Dry Season Tomato Production in Ikara Local Government Area of Kaduna State, Nigeria. The Nigerian Journal of Energy and Environmental Economics (NJEE). Vol: 11 (1) pp103-113.
Aremu, F.J, Adeyemo, R and Olugbire, O.O (2016). Economic Analysis of Fresh Tomato Marketing in the Wet Season under Tropical Conditions. Journal of Sustainable Development. Vol: 13(1) pp 65-72.
Asogwa, B. C. Ihemeje, J. C. and Ezihe, J. A. C. (2011). Technical and Allocative Efficiency Analysis of Nigerian Rural Farmers: Implication for Poverty Reduction, Agricultural Journal. Vol: 6(5): pp 243-251.
Benjamin, C. A, Simon, T. P. and Wuraola, L. L. (2011). Application of Data Envelopment Analysis to Evaluate Farm Resource Management of Nigerian Farmers. Journal of Agricultural Science. Vol: 2(1): pp9-15.
Christine Plaisier, Youri Dijkxhoorn, Fédes van Rijn, Johann Bonnand and Olufolajimi Talabi, 2019. The Vegetable and Potato Sector Nigeria; An overview of the present status. Wageningen, Wageningen Economic Research, Report 2019-119. Pp. 68.
Danmaigoro, A. and Gona, A. (2020). Tomato Production under Irrigation in Some Selected Districts of Zuru Local Government Area of Kebbi State, Nigeria. Journal of Environmental and Agricultural Studies. Vol: 1(1):pp 11-16.
Food and Agricultural Organization (FAO) (2019). Nigeria Country Profile. Retrieved from http://faostat.fao.org/static/syb/syb_159.pdf (23-4-2019).
Federal Ministry of Agriculture and Rural Development (FMARD) (2015). ‘Tomato Action Plan for Nigeria 2015- 2019’, Horticulture Division, Federal Department of Agriculture, FMARD Nigeria.
Haruna, U. (2004). Prospects and Problems of Vegetable Production under Irrigation in the Fadama Areas of Bauchi State, Nigeria. Horticultural Society of Nigeria, Proceedings of the 22nd Annual Conference.
Hadejia Jama’are River Basin Development Authority (HJRBDA, 2013). Brief on Kano River Irrigation Project (KRIP), Phase I. Kano State Government (2012). Brief on Kano State. kano.gov.ng/new/index.php/2012-02- 28-03-51-05/brief. Accessed on 10th January, 2013.
Katanga, Y.N., Danwawu, S.S and Musa, B. (2018). Economic Analysis of Tomato Production in Fagge Local Government Kano State, Nigeria. Dutse Journal of Economics and Development Studies (DUJEDS). Vol: 6(2): pp 98-104.
Lenis, S. L, Oluyemisi, K. and Akeem, A. (2011). A Review of Literature on Agricultural Productivity, Social, Capital and Food Security in Nigeria: National Strategy Support Programme (NSSP), International Food Policy Research Institute (IFPRI), Washington D. C, USA. NSSP Working Paper No. 21.
Olanrewaju, D. and Swarup, V. (1983).Tomato Cultivation and it’s Potential in Nigeria. National Horticultural Research Institute, Idi-Ishin, Ibadan, Nigeria, Occasional Paper No.
Usman, J. and Bakari, U.M. (2013).Profitability of Small Scale Dry Season Tomato (Lycopersicon esculentum Mill.) Production in Adamawa State, Nigeria. ARPN Journal of Science and Technology. Vol: 3 (6): pp 604-612.
Published
How to Cite
Issue
Section
FUDMA Journal of Sciences
