Bacterial Contamination and Antimicrobial Resistance Profiles of Street-Vended Foods in Ogwa and Ebelle, Edo State, Nigeria
DOI:
https://doi.org/10.33003/fjs-2026-1019-6159Keywords:
Antimicrobial resistance, Street foods, Multidrug resistance, Food safetyAbstract
Street foods provide affordable and accessible nutrition but may serve as reservoirs of pathogenic and antimicrobial-resistant (AMR) bacteria. This study investigated bacterial contaminants and antimicrobial resistance patterns among bacteria isolated from commonly consumed street foods sold in Ogwa and Ebelle communities, Edo State, Nigeria. Twenty street food samples comprising rice-based (n=8), bread/pastry-based (n=5), and yam/plantain-based foods (n=7) were analyzed using standard microbiological techniques. Bacterial isolates were identified by colony morphology, Gram staining, biochemical characterization, and polymerase chain reaction (PCR) detection of selected resistance genes. Antimicrobial susceptibility was determined using the Kirby–Bauer disk diffusion method following CLSI guidelines. Ten bacterial isolates comprising Staphylococcus aureus, Escherichia coli, Pseudomonas spp., and Salmonella spp. were recovered. Rice-based foods recorded the highest mean total viable count (537 ± 42 × 10³ CFU/g; P=0.018). All 10 isolates were resistant to ceftriaxone, ampiclox, and amoxicillin. Multidrug resistance was observed in 9 of 10 isolates (90%), based on resistance to at least one antimicrobial agent in three or more antimicrobial classes. PCR detected mecA, blaTEM, blaCTX-M, tetA, and sul1 among MDR isolates, while blaSHV was not detected. These findings highlight the need for improved food hygiene, microbiological surveillance, and antimicrobial stewardship.
References
Adane, M., Teka, B., Gismu, Y., Halefom, G., & Ademe, M. (2018). Food hygiene and safety measures among food handlers in street food shops and food establishments of Dessie Town, Ethiopia: A community-based cross-sectional study. PLoS ONE, 13(7), e0196919. https://doi.org/10.1371/journal.pone.0196919
Adeleke, O. A., & Owoseni, A. A. (2022). Antibiotic resistance and presence of plasmids in bacteria isolated from cooked street foods. International Journal of Environmental Studies, 80(3), 664–671. https://doi.org/10.1080/00207233.2022.2111149
Adhikari, S., Regmi, R. S., Sapkota, S., Khadka, S., Patel, N., Gurung, S., Thapa, D., Bhattarai, P., Sapkota, P., Devkota, R.. (2023). Multidrug resistance, biofilm formation and detection of blaCTX-M and blaVIM genes in Escherichia coli and Salmonella isolates from chutney served at street-food stalls of Bharatpur, Nepal. Heliyon, 9, e15739.
Ahmad, N., Joji, R. M., & Shahid, M. (2023). Evolution and implementation of One Health to control the dissemination of antibiotic-resistant bacteria and resistance genes: A review. Frontiers in Cellular and Infection Microbiology, 12, 1065796.
Akinyele, H. A., Oluwaseun, A. A., Tunde, A. K., & Oluwaseun, A. C. (2024). Detection and antibiogram of bacterial contaminants in some commonly consumed indigenous street vended foods in Kasua Central Market in Kaduna North, Kaduna State, Nigeria. Dutse Journal of Pure and Applied Sciences, 10(1a), 100–110. https://doi.org/10.4314/dujopas.v10i1a.11
Anukampa, Shagufta, B., Sivakumar, M., Kumar, S., Agarwal, R. K., Bhilegaonkar, K. N., Kumar, A., & Dubal, Z. B. (2017). Antimicrobial resistance and typing of Salmonella isolated from street-vended foods and associated environment. Journal of Food Science and Technology, 54(8), 2532–2539. https://doi.org/10.1007/s13197-017-2682-6
Avoseh, D. O., Okanlawon, T. S., Ekozin, A. A., Okanlawon, K. P., & Olatunji, E. O. (2024). Assessing the water quality of River Obazagbon, Edo State, Nigeria using physicochemical and bacteriological parameters. Pollution, Bioremediation & Biodegradation Journal, 5(1), 180020. https://doi.org/10.23880/pbbj-16000180
Baloch, A. B., Yang, H., Feng, Y., Xi, M., Wu, Q., Yang, Q., Tang, J., He, X., Xiao, Y., & Xia, X. (2017). Presence and antimicrobial resistance of Escherichia coli in ready-to-eat foods in Shaanxi, China. Journal of Food Protection, 80(3), 420–424. https://doi.org/10.4315/0362-028X.JFP-16-262
Cheesbrough, M. (2009). District laboratory practice in tropical countries (2nd ed.). Cambridge University Press.
Dela, H., Egyir, B., Behene, E., Sulemana, H., Tagoe, R., Bentil, R., Bongo, R. N. A., Bonfoh, B., Zinsstag, J., Bimi, L., et al. (2023). Microbiological quality and antimicrobial resistance of bacteria species recovered from ready-to-eat food, water samples, and palm swabs of food vendors in Accra, Ghana. International Journal of Food Microbiology, 396, 110195.
Ghali-Mohammed, I., Azeez, A. A., Akeem, A. O., Adetona, M. A., Adeyemo, I. A., Olawoye, O. C., Alhaji, N. B., & Odetokun, I. A. (2025). Assessment of keeping quality and microbial load of Staphylococcus aureus in cattle milk, processing facilities, and environment in Northern Oyo State, Nigeria. FUDMA Journal of Sciences, 9(3), 309–315. https://doi.org/10.33003/fjs-2025-0903-3016
Gulati, D., & Chakraborty, D. (2017). Antibiogram of bacterial pathogens isolated from one of the most popular street food (Panipuri) of Dehradun. International Journal of Pharma and Bio Sciences, 8(3), 395–400. https://doi.org/10.22376/ijpbs.2017.8.3.395-400
Kintum, A. T., Oyetibo, O. B., Lanlokun, O. A., & Omemu, A. M. (2025). Microbial profile of ready to eat meat and its preparation environment in Lagos State, Nigeria. Scientia Africana, 24(5), 59–70. https://doi.org/10.4314/sa.v24i5.8
Ma, L., Chen, H., Yan, H., Wu, L., & Zhang, W. (2019). Food safety knowledge, attitudes, and behavior of street food vendors and consumers in Handan, a third tier city in China. BMC Public Health, 19, 1128. https://doi.org/10.1186/s12889-019-7426-0
Makinde, O. M., Akinyemi, M. O., Bezuidenhout, C., Adeleke, R. A., & Ezekiel, C. N. (2026). Antibiogram of isolated bacteria and overview of resistome and virulome of multi-drug resistant Escherichia coli from ready-to-eat foods vended in Lagos, Nigeria. BMC Microbiology. https://doi.org/10.1186/s12866-025-04694-x
Nosa, A. A., Okanlawon, T. S., Onyijen, O. H., Iyafokhia, I. U., Ekozin, A. A., Isola, S. E., & Omojoyegbe, R. T. (2024). Bioinformatics-driven analysis and antibiotic resistance profiling of Enterobacter species isolated from acne lesions. Science World Journal, 19(4), 1202–1209. https://doi.org/10.4314/swj.v19i4.39
Nwajiobi, F. O., Ogu, C. T., Anene, C. C., Ugwu, C. H., & Chukwujekwu, A. G. (2025). Molecular detection and antimicrobial resistance profiles of multidrug-resistant Escherichia coli in ready-to-eat foods from mobile vendors in Awka, Nigeria. Journal of Disease and Global Health, 18(2), 202–209. https://doi.org/10.56557/jodagh/2025/v18i29678
Ogunremi, O. R., Ukpong, E. S., & Nosa, A. A. (2021). Analysis and antibiogram study of microorganisms associated with fish and meat retail surfaces in Ebelle, Nigeria. Food and Environment Safety, 20(4).
Okanlawon, , T., Olarinre, M., Tobby, U., Oluwasade, A., Ogunniran, J., Ogunrinade, . J., Olayori, A. O., Oladeji, D., Oni, J., Adeyemi, . A., Hamzat, . A. ., & Oladipo, . C. (2026b). Microbial Bioprospecting and Antibacterial Potential of Natural Products Against Multidrug-Resistant Pathogens: A Review. BIMA JOURNAL OF SCIENCE AND TECHNOLOGY GOMBE, 10(2), 420-432. https://doi.org/10.64290/bima.v10i2.1622
Okanlawon, T. S., Adeyemo, S. M., & Agbaje, I. S. (2023). Isolation and identification of microorganisms associated with Jollof rice sold at Bukateria in Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria. GSC Biological and Pharmaceutical Sciences, 22(1), 178–185. https://doi.org/10.30574/gscbps.2023.22.1.0029
Okanlawon, T. S., Alabi, O. O., Obi, C. G., Omobolade, E. S., Omobolade, O., Otun, M. O., Badmus, A. A., Akinsola, O. S., & Akinyemi, K. I. (2025b). Antibacterial activity of Allium sativum (garlic) against bacterial isolates from urine samples. Journal of Pharmaceutical and Allied Sciences, 22(1), 4595–4608.
Okanlawon, T. S., Ehijele, D., Okanlawon, K. P., Omobolade, E. S., Agbaje, I. S., Akinsola, O. S., & Unogwa, G. C. (2024a). Isolation, characterization and optimization of pigment-producing bacteria from different sources in Ogwa, Esan West Local Government Area, Edo State, Nigeria. Journal of Applied Sciences and Environmental Management, 28(10B Supplementary), 3407–3412. https://doi.org/10.4314/jasem.v28i10.57
Okanlawon, T. S., Fagbohun, A., Isokpehi, N. A., Otun, M. O., Kukoyi, O. A., Unogwa, G. C., Omobolade, E. S., & Omobolade, O. (2025a). Isolation, characterization and antibiotic susceptibility profile of bacteria isolated from fomites. BIMA Journal of Science and Technology, 9(3B), 37–49. https://doi.org/10.64290/bima.v9i3B.1362
Okanlawon, T. S., Okanlawon, K. P., Ekozin, A. A., Unogwa, G. C., Fasanya, O. S., Ighodalo, A. J., & Akinsola, O. S. (2024b). Physicochemical, bacteriological and enzymatic assessment of water reservoirs in Ogwa and Ebelle communities, Edo State, Nigeria. Journal of Applied Sciences and Environmental Management, 28(10), 3463–3469. https://doi.org/10.4314/jasem.v28i10.63
Okanlawon, T. S., Olarinre, M. A., Hamzat, A. V., Ogunrinade, J. O., Oladeji, D. O., Ogunniran, J. O., Oni, J. O., Tobby, U. E., Olayori, A. O., Samuel, I. O., & Oluwasade, A. M. (2026a). Bioprospecting enzyme-producing and antimicrobial microbes from local environments: Opportunities for biotechnology and public health innovation. BIMA Journal of Science and Technology, 9(4B), 106–117. https://doi.org/10.64290/bima.v9i4B.1449
Oladeji, Y. G., Adedire, S. A., Okanlawon, T. S., Adebomi, O. H., & Bisi-Johnson, A. M. (2024). Antibiotics susceptibility pattern of Pseudomonas aeruginosa isolated from Obafemi Awolowo University Ile-Ife oxidation pond. International Journal of Basic Science and Technology, 10(1), 81–90. https://doi.org/10.5555/VIWH1609
Olatunji, E. O., Isola, O. B., & Okanlawon, T. S. (2022). Identification of spoilage bacteria from fermented food products in Edo State, Nigeria. Journal of Food Microbiology, 6(5), 1–10.
Sandrasaigaran, P., Kuan, C. H., Radu, S., Abidin, U. F. U. Z., Rukayadi, Y., New, C. Y., & Hasan, H. (2023). Multiple antibiotic-resistant Salmonella Enterica serovars Enteritidis and Typhimurium in ready-to-eat battered street foods, and their survival under simulated gastric fluid and microwave heating. Food Control, 146, 109515. https://doi.org/10.1016/j.foodcont.2023.109515
Tacardon, E. R., Ong, A. K. S., & Gumasing, M. J. J. (2023). The perception of food quality and food value among the purchasing intentions of street foods in the capital of the Philippines. Sustainability, 15(17), 12549. https://doi.org/10.3390/su151712549
Tadesse, G., Mitiku, H., Teklemariam, Z., & Marami, D. (2019). Salmonella and Shigella among asymptomatic street food vendors in the Dire Dawa city, Eastern Ethiopia: Prevalence, antimicrobial susceptibility pattern, and associated factors. Environmental Health Insights, 13, 1178630219853581. https://doi.org/10.1177/1178630219853581
Usman, R. A., & Umar, S. M. (2025). Evaluation of the microbiological quality of indigenous complementary foods in Kano State. FUDMA Journal of Sciences, 9(11), 401–406. https://doi.org/10.33003/fjs-2025-0911-4210
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Copyright (c) 2026 Taiwo Okanlawon, Destiny Isidahomen, Opeoluwa Abosede Akindowo, Olorunfemi Omobolade, Nnenna Amarachi Isokpehi, Efe Oviawe
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