PHENOTYPIC DETECTION OF EXTENDED SPECTRUM BETA-LACTAMASE (ESBL) PRODUCING Escherichia coli FROM LIVESTOCK AND HERDERS IN SOME STATES IN NORTH-EASTERN NIGERIA
DOI:
https://doi.org/10.33003/fjs-2026-1007-5038Keywords:
Phenotypic Detection, Extended Spectrum Beta-Lactamase (ESBL), Escherichia coli, Livestock and HerdersAbstract
The emergence and dissemination of ESBL producing bacteria of food-animals and humans origin are responsible for numerous infections with challenging treatment and control measures which calls for considerable concern of both Medical and Veterinary practitioners all over the World. Phenotypic methods of ESBL screening are widely used for their simplicity, cost-effectiveness and suitability for routine clinical laboratories. The aim of this study was to isolate MDR E. coli isolates and screen them phenotypically for ESBL production using the Double Disk Synergy Test (DDST). A total of 1152 samples comprising of 384 each from cattle sheep and Herders were collected, processed, inoculated and incubated at 37 oC for 24 hours. One hundred and eighty one (181) samples yielded positive bacterial growths out of which 111 (61.3%) of them were E. coli isolates and 70 (%) were other members of the gram negative bacilli. Out of the 111 E. coli isolates, 91 (81.9%) were multidrug resistant of which 37 (36.3%) were phenotypically detected to be an ESBL producing phenotypes. This study revealed significant difference (P value < 0.05) between the E. coli and other members of the gram negative bacilli isolated. Calves, lambs and young herders below 10 years of age found in closed–contact with livestock as well as those living in an over-crowded and dirty environment were more prevalent to infection by ESBL producing bacteria. Therefore, proper sanitary measures be adopted and maintained in farms where calves and Lambs less than 1 year are kept. Young herders’ below10 years old...
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