HAEMATOLOGICAL RESPONSE OF Clarias gariepinus (BURCHELL, 1822) FINGERLINGS TO VARYING LEVELS OF BETAINE/B – GLUCAN FEED ADDITIVE

Authors

  • Mark Eka Ali
    National Biotechnology Research and Development Agency Gombe
  • Olukayode Amos Sogbesan
    Modibbo Adama University, Yola
  • Kotos Abdulrahman Abubakar
    Modibbo Adama University, Yola
  • Sylvia Ali Salisu
    National Biotechnology Research and Development Agency Gombe

Keywords:

Haemoglobin, Lymphocytes, Monocytes, Packed Cell Volume, Platelet

Abstract

This study was designed to evaluate the haematological response of Clarias gariepinus fingerlings to varying levels of betaine/ glucan feed additive. Combination of betaine/ glucan as feed additive was included into formulated feed with 40% crude protein at; 0.0g/100g (BBG0 i.e. control), 0.325g/100g (BBG1), 0.75g/100g (BBG2), 1.125g/100g (BBG3) and 1.50g/100g (BBG4) in triplicates. Feed was fed to C. gariepinus fingerlings (n = 300, 10.00.00g) in fifteen (15) plastic tanks (n = 20) at a fixed feeding rate of 3% body weight twice daily between the hours of 8:00 9:00am and 4:00 5:00pm at regular interval and adjusted after every two (2) weeks of sampling for a period of twelve (12) weeks. Blood samples were collected from three (3) fish at the commencement and also from three (3) fish from each treatment and the control at the termination of the feeding trial for hematological analysis. Data collected was statistically analysed using one - way analysis of variance (ANOVA) at P = 0.05. PCV values (41.33.3 42.71.9 %), RBC (2.560.6 106 2.820.1 106 dL-1), WB (249.814 103 266.5 16 103 dL-1), Hb (12.12.2 13.52.4 gdL-1), platelet (27.751.5 104 29.201.4 104 dL-1), MCV (136.77.9 155.111.0 m-3), MCH (40.72.2 50.12.5 gL-1), MCHC (31.22.1 33.12.6 gdL-1), neutrophil (32.103.2 33.702.0 %), lymphocyte (95.76.4 97.68.3 %) and monocytes (2.850.2 3.700.3 %) respectively were recorded. Combination of betaine/ glucan feed additive at varying levels used in this study did not result in statistically significant changes (p>0.05) in haematological responses of C. gariepinus.

Dimensions

Adesina, S. A. (2017). Haematological and serum biochemical profiles of Clarias gariepinus juveniles fed diets containing different inclusion levels of mechanically extracted sunflower (Helianthus annuus) seed meal. Journal of Applied Tropical Agriculture, 22 (2): 24 35.

Agbabiaka, L. A., Atanda, A. O. and Nwankpa, R. (2016). Growth and haematological characteristics of African catfish (Clarias gariepinus, Burchel, 1822) fed African star apple kernel diets. International Journal of Zoology Studies, 1(3): 25 29.

Akinde, A.O., Joda, O. A. and Ibidapo - Obe, E. O. (2020). Growth performance, haematology and intestinal microflora of Clarias gariepinus (Burchell, 1822) fingerlings fed turmeric (Curcuma longa) supplemented diets. Proceedings of the 35th Annual Conference of Fisheries Society of Nigeria (FISON). Pp. 97 - 102.

Akinrotimi, O. A., George, A. and Nwokoma, U. K. (2017). Haematological changes in African catfish (Clarias gariepinus) exposed to mixture of atrazine and metolachlor in the laboratory. Journal of FisheriesSciences.com, 11(3): 48 54.

Ali, M. E., Danba, E. P., Sani, T., and Danzaria, A. (2024). Evaluation of the carcass proximate composition of Clarias gariepinus (Burchell, 1822) fingerlings fed different levels of maca (Lepidium meyenii, Walp.) root powder as phyto additive. African Journal of Agricultural Science and Food Research (AJASFR), 15(1): 107 117.

Anyanwu, C. N., Osuigwe, D. I., Nwaka, D. E., Anyanwu, D. C. and Chukwuotito, P. C. (2017). Evaluation of growth and body composition of Clarias gariepinus fingerlings fed graded levels of Enterococcus faecium. Proceedings of the 32nd Annual Conference of Fisheries Society of Nigeria (FISON). Pp. 16 19.

Ayoola, S. (2011). Haematological characteristics of Clarias gariepinus (Buchell, 1822) juveniles fed with poultry hatchery waste. Iranica Journal of Energy and Environment, 2(1): 18 23.

Babale, M. Y. (2016). Growth performance at various growth stages of Clarias gariepinus (Burchell, 1822) fed various inclusion levels of processed water melon (Citrullus lanatus, mansf.) seed cake diets. Published Ph.D. Thesis, Department of Biological Sciences, Ahmadu Bello University Zaria, Zaria, Nigeria. Pp. 164.

Bichi, K. A., Yusif, B. B., Mustapha, S. B., Jibrin, Y. U. and Thomas, P. S. (2021). Evaluation of hematological parameters in albino rats exposed dermally to tannery effluent from Challawa industrial estate Kano, Nigeria. FUDMA Journal of Sciences (FJS), 5(1): 372 376.

Bittencourt, N. L. R., Molinari, L. M., Scoaris, D. O., Pedroso, R. B., Nakamura, C. V., Ueda - Nakamura, T., Filho, B. A. A. and Filho, B. P. D. (2003). Haematological and biochemical values for Nile tilapia Oreochromis niloticus cultured in semi - intensive system. Acta Scientiarum Biological Sciences, 25 (2): 385 389.

Elezuo, K. O. (2016). Growth performance OF Clarias gariepinus juveniles fed processed almond (Terminalia catappa L.) kernel meal. Ph.D. Thesis, Department of Aquaculture and Fisheries Management, University of Ibadan, Ibadan, Nigeria. Pp. 197.

Inya, F. U., Okey, I. B., Ochang, S. N. and Ekpenyong, J. J. (2020). Effects of replacing soybean with beniseed, (Sesame indicum) seed meal on some haematological parameters of Clarias gariepinus juveniles. Proceedings of the 35th Annual Conference of Fisheries Society of Nigeria (FISON). Pp. 75 80.

Lawal, M. O., Aderolu, A. Z. and Nwobodo, T. U. (2016). Effects of stoolwood (Alstonia boonei), Hogplum (Spondias mombin) and brimstone (Morinda lucida) leaves extracts on growth and haematology of catfish, Clarias gariepinus juveniles. Proceedings of the 31st Annual Conference of Fisheries Society of Nigeria (FISON). Pp. 156 159.

Melefa, T. D. and Okoloye, U. (2023). Haematological and biochemical profile of Clarias gariepinus from Opi lake in Nsukka local government area, Enugu state. FUDMA Journal of Sciences (FJS), 7(6): 391 395.

Nya, E. J. (2009). Studies on dietary supplements for the control of Aeromonas hydrophila infection in rainbow trout (Oncorhynchus mykiss, Walbaum). Published Ph.D. Thesis, Centre for Marine Biodiversity and Biotechnology, Heriot Watt University, Edinburgh, UK. Pp. 179.

Ofonime, E. A. and David, G. S. (2017). Haematological profile and growth response of African sharptooth catfish (Clarias gariepinus, Burchell, 1822) fingerlings to locally formulated and commercial pelleted diets in tarpaulin tanks. American Journal of Innovative Research and Applied Sciences, 4(6): 213 222.

Olasunkanmi, J. B. (2011). Nutrient utilization and growth performance of Clarias gariepinus fed differently processed Mucuna utilis meals as a replacement for soya bean - based diet. Published Ph.D. Thesis, Department of Wildlife and Fisheries Management University of Ibadan, Ibadan, Nigeria. Pp. 195.

Oshode, O. A. I.., Bakare, A. A.., Adeogun, A. O., Efuntoye, M. O. and Sowunmi, A. A. (2008). Ecotoxicological assessment using Clarias gariepinus and microbial characterization of leachate from municipal solid waste landfill. International Journal of Environmental Research, 2(4): 391 400.

Osuigwe, D. I., Obiekezie, A. I. and Onuoha, G. C. (2005). Some haematological changes in hybrid catfish (Heterobranchus longifilis x Clarias gariepinus) fed different dietary levels of raw and boiled jackbean (Canavalia ensiformis) seed meal. African Journal of Biotechnology. 4 (9): 1017 1021.

Robinson, E. H., Li, M. H. and Manning, B. B. (2001). A practical guide to nutrition, feeds and feeding of catfish. Bulletin 1113. Mississippi Agriculture and Forestry Experiment Station, Mississippi State University, Starkville, Mississippi, U.S.A.

Shah, S. L. and Altindag, A. (2004). Haematological parameter of tench (Tinca tinca) after acute and chronic exposure to lethal and sub - lethal mercury treatments. Bulletin of Environmental Contamination Toxicology, 13(73): 911 918.

Soyinka, O. O. and Boafo, F. O. (2015). Growth performance, haematology and biochemical characteristics of Clarias gariepinus (Burchell, 1822) juveniles fed quail eggshells as replacement for dicalcium phosphate. Nigerian Journal of Fisheries and Aquaculture, 3(1&2): 49 54.

Teixeira, M. A. (2000). Haematological and bio - chemical profiles of rats (Rattus norvegicu) kept under micro - environmental ventilation system. Brazilian Journal of Veterinary Resources and Animal Science, 37:50 55.

Terry, C., Hrubec, T. C. and Stephen, A. S. (2000). Haematology of fish. Journal of Veterinary Clinical Pathology, 23(174): 1120 1125.

Tiamiyu, A. M., Olatoye, I. O. and Adedeji, O. B. (2019). Blood indices of African catfish (Clarias gariepinus) following dietary administration of Talinum triangulare. International Journal of Research - Granthaalayah, 7:4: 185 198. ISSN - 2350-0530 (O), ISSN-2394-3629(P) DOI: https://doi.org/10.29121/granthaalayah.v7.i4.2019.888

Udoh, J. P., Emah, A. U., George, I. E. and Philip, A. E. (2017). Growth performance and haematological response of Clarias gariepinus broodstock fed diets enriched with bitter leaf meal. AACL Bioflux, 10(5): 1281 1296.

Published

29-04-2025

How to Cite

HAEMATOLOGICAL RESPONSE OF Clarias gariepinus (BURCHELL, 1822) FINGERLINGS TO VARYING LEVELS OF BETAINE/B – GLUCAN FEED ADDITIVE. (2025). FUDMA JOURNAL OF SCIENCES, 9, 246-251. https://doi.org/10.33003/fjs-2025-09(AHBSI)-3480

Issue

Vol. 9 (2025): FUDMA Journal of Sciences - Vol. 9 April (AHB Special Issue)

Section

Research Articles
Copyright & Licensing

How to Cite

HAEMATOLOGICAL RESPONSE OF Clarias gariepinus (BURCHELL, 1822) FINGERLINGS TO VARYING LEVELS OF BETAINE/B – GLUCAN FEED ADDITIVE. (2025). FUDMA JOURNAL OF SCIENCES, 9, 246-251. https://doi.org/10.33003/fjs-2025-09(AHBSI)-3480