ASSESSMENT OF SELECTED PLANT EXTRACT AS EXTENDER ON SPERM VIABILITY AND FUNCTIONAL INTEGRITY OF RED SOKOTO BUCK DURING COLD STORAGE

Authors

Keywords:

Cold storage, Plant extract, Red Sokoto buck, Semen extender, Sperm viability

Abstract

The use of plant-based extenders has gained interest due to their protective and antioxidant capacity. This study evaluates the effects of scent leaf (Ocimum gratissimum), onions (Allium cepa), lemongrass (Cymbopogon nardus), and celery (Apium graveolens) extracts on the viability and functional integrity of Red Sokoto buck semen during cold storage. Semen samples were collected from ten mature Red Sokoto buck, pooled, and diluted with tris egg yolk extender. In a completely randomized design, the pooled semen samples were divided into 5. The different plant extracts were added to each sample at 1.5ml/ 1 ml of semen sample with the exception of the control which only contain tris egg yolk extender. Samples were then stored at 4°C in the refrigerator for 7 days. After which it was evaluated for sperm viability (motility, livability and abnormalities) and sperm functional integrity (membrane integrity, acrosome integrity and semen concentration). The result showed no significant difference (P>0.05) in motility, livability, or functional integrity among the treatments. However, scent leaf and lemongrass exhibited a higher sperm motility and livability, while celery showed the lowest. Lemongrass extract had higher acrosome and membrane integrity, whereas onions showed the lowest. Sperm abnormalities were reduced across treatments, supporting the protective role of plant extracts in semen storage. This study suggests that plant-based extenders are effective in semen preservation and therefore may serve as cost-effective and bio secured alternatives to synthetic extenders, sustaining the quality of semen during cold storage and enhancing reproductive efficiency in Red Sokoto goats.

Dimensions

Adewole, K. E., & Attah, A. F. (2020). Antimalarial plants with potential male-factor antifertility properties. Journal of Complementary and Integrative Medicine, 17(2), 20180214.

Ahmad, M., Nasrullah, R., Riaz, H., Sattar, A. & Ahmad, N., 2014. Changes in motility, morphology, plasma membrane and acrosome integrity during stages of cryopreservation of buck sperm. Journal of Association, 85(1), 1-4.

Ali, E. A., & Abdel-Zalmi, S. S. (2022, July). Effect of Adding Different Levels of Aqueous Extract of Lemongrass Leaves on Reproductive Characteristics, Testis Weight and Testicular Tissues of Male Broiler Mothers ROSS308. In IOP Conference Series: Earth and Environmental Science (Vol. 1060, No. 1, p. 012074). IOP Publishing.

Alrubaie, N. H. M., Alyasiri, E. A., & Mohammed, O. A. (2022). Efficacy of Nigella sativa extract on abnormalities of cryopreserved sperm of Holstein bulls raised in Iraq. International Journal of Health Sciences, 6(S4), 52025209. https://doi.org/10.53730/ijhs.v6nS4.9316

Bassuony, N. I., Assi, M. M., Sakr, A. A., Bakr, A. A., Anwar, D. A., & Badr, M. R. (2023). Effect of Bioactive Compounds of Lemon, Onion and Garlic Extract on Buffalo Semen Quality, Antioxidant Status and Ultrastructure Changes. Alexandria Journal of Veterinary Sciences, 76(1).

Boroujeni, S. N., Malamiri, F. A., Bossaghzadeh, F., Esmaeili, A., & Moudi, E. (2022). The most important medicinal plants affecting sperm and testosterone production: A systematic review. JBRA assisted reproduction, 26(3), 522.

Bucak, M. N., & Tekin, N. (2007). Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Research, 73(1-3), 103-108.

Cecere, J. T. (2014). EosinNigrosin staining in the evaluation of sperm. In: Dascanio, J. J., & McCue PM. Equine reproductive procedures (pp. 373-376). Wiley Online Library.

Chavda, V. P., Sonak, S. S., Balar, P. C., Vyas, K., Palandurkar, P., Mule, K., & Apostolopoulos, V. (2024). Reviving Fertility: Phytochemicals as Natural Allies in the Fight against Non-genetic Male Infertility. Clinical Complementary Medicine and Pharmacology, 4(1), 100128.

Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics, 11(1), 1-42.

Guerrero-Guzmn, A., Villarreal-Pavn, F., Zamudio-Ojeda, A., Velzquez-Jurez, G., Ramos-Ibarra, R., Snchez-Toscano, Y. G., & Snchez-Chiprs, D. R. (2021). Antioxidant effect of Cymbopogon citratus and Hypericum perforatum extracts in boar semen like a new alternative for conservation. All Life, 14(1), 954-962.

Hufana-Duran, D., Mallari, R. P., Suba, D. P., Duran, P. G., Abella, E. A., & Mamuad, F. V. (2015). Hypo-osmotic swelling test for membrane integrity evaluation of frozen-thawed water buffalo (Bubalus bubalis Linn.) spermatozoa. Philippine Journal of Science, 144(2), 209-219.

Imosemi, I. O. (2020). A review of the medicinal values, pharmacological actions, morphological effects and toxicity of Ocimum gratissimum Linn. Eur J Pharm Med Res, 7(7), 29-40.

Kooti, W., Moradi, M., Peyro, K., Sharghi, M., Alamiri, F., Azami, M., & Ghafourian, M. (2018). The effect of celery (Apium graveolens L.) on fertility: A systematic review. Journal of Complementary and Integrative Medicine, 15(2).

Kowalczyk, A., Gaska, E., & Bubel, A. (2022). The concentration of ProAKAP4 and other indicators of cryopotential of spermatozoa cryopreserved in extender with Holothuroidea extract addition. Animals, 12(4), 521.

Kuralkar, P., & Kuralkar, S. V. (2021). Role of herbal products in animal productionAn updated review. Journal of Ethnoparmacology, 278, 114246.

Madkour, N. K. (2014). Beneficial role of celery oil in lowering the di (2-ethylhexyl) phthalate-induced testicular damage. Toxicology and industrial health, 30(9), 861-872.

Mphaphathi, M. L., Sebopela, M. D., Ledwaba, M. R., Thema, M. A., Maapola, R., Negota, N. C., & Netshirovha, T. R. (2024). The Use of Plants Extracts during Cryopreservation of Livestock Epididymal Semen.

Shokoohi, M., Madarek, E. O. S., Khaki, A., Shoorei, H., Khaki, A. A., Soltani, M., & Ainehchi, N. (2018). Investigating the effects of onion juice on male fertility factors and pregnancy rate after testicular torsion/detorsion by intrauterine insemination method. Int J Womens Health Reprod Sci, 6(4), 499-505.

SPSS (2000). Student Version 9.0 for Windows. Journal of English Linguistics, 28(3), 311-313.

Tanga, B. M., Qamar, A. Y., Raza, S., Bang, S., Fang, X., Yoon, K., & Cho, J. (2021). Semen evaluation: Methodological advancements in sperm quality-specific fertility assessmentA review. Animal bioscience, 34(8), 1253.

Walke, G., Gaurkar, S. S., Prasad, R., Lohakare, T., & Wanjari, M. (2023). The impact of oxidative stress on male reproductive function: exploring the role of antioxidant supplementation. Cureus, 15(7).

Yahya, M. M., & Midau, A. (2023). Reproductive Performance of Red Sokoto goat breed in Adamawa State. Fudma journal of sciences, 7(3), 60-63.

Published

26-04-2025

How to Cite

ASSESSMENT OF SELECTED PLANT EXTRACT AS EXTENDER ON SPERM VIABILITY AND FUNCTIONAL INTEGRITY OF RED SOKOTO BUCK DURING COLD STORAGE. (2025). FUDMA JOURNAL OF SCIENCES, 9, 1-4. https://doi.org/10.33003/fjs-2025-09(AHBSI)-3370

How to Cite

ASSESSMENT OF SELECTED PLANT EXTRACT AS EXTENDER ON SPERM VIABILITY AND FUNCTIONAL INTEGRITY OF RED SOKOTO BUCK DURING COLD STORAGE. (2025). FUDMA JOURNAL OF SCIENCES, 9, 1-4. https://doi.org/10.33003/fjs-2025-09(AHBSI)-3370

Most read articles by the same author(s)