ISOLATION AND IDENTIFICATION OF FUNGI ASSOCIATED WITH SOILS AND DISEASED TOMATO PLANTS ON FARMERS’ FIELDS IN BENUE STATE NIGERIA

Authors

Keywords:

Fungi, Identification, Infected Soils, Isolation, Tomato Plants

Abstract

A study was carried out to isolate and identify fungi associated with infected tomato plants and soils collected from tomato fields in major tomato growing Local Government Areas (LGAs) of Benue State, Nigeria. Fungi isolated from diseased tomato plant tissues and rhizospheric soils using the direct plate and soil dilution techniques respectively were identified through examination of cultural and microscopic features, aided by relevant identification keys and manuals. A total of 46 fungi belonging to 5 divisions and 14 genera were isolated from sampled plant and soil materials. Soil samples gave the highest number of fungal isolates, 31/46(67.39%) compared to plant materials, 15/46(32.61%). Plant and soil samples collected from Buruku LGA yielded the highest number of fungal isolates, 16 followed by Ushongo, 14, Gboko, 10, and lastly Tarka, 06. Fungi belonging to the Division Deuteromycota were the most frequently occurring, 76.09%, followed by Ascomycota, 13.04% and both Oomycota and Basidiomycota with 4.35% respectively. Members of the Zygomycota were the least occurring in the sampled materials with 2.17%. Fungi belonging to the genus Aspergillus were the most frequently occurring with 19.57%. The least frequently occurring fungi were those belonging to the genera Colletotrichum, Scopulariopsis, and Mucor, all having 2.17% frequencies of occurrence respectively. The study revealed the occurrence of fungi in tissues and rhizosphere of the tomato plant, which included several fungi genera of agricultural importance.

Dimensions

Bem, A.A. (2009). Incidence of Major Fungal Diseases of Tomato (Lycopersiconesculentum L.) and their Evaluation in some Tomato Growing Areas of Benue State, Nigeria. Ph.D. Thesis submitted in the Department of Biological Sciences, University of Agriculture, Makurdi, Nigeria. Pp.1-231.

Bjarnadottir, A. (2019). Tomatoes 101: Nutrition, Facts, and Health Benefits. Available online at: https://www.healthline.com/nutrition/foods/tomatoes. Accessed on: 21/6/2019.

Bollen, W.B. (1959). Microorganisms and Soil Fertility. Oregon: CORVALLIS. Pp.6.

Brady, N.C., and Weil, R.R. (2002). The nature and properties of soils, 13th Editionn. Upper Saddle River, NJ: Prentice Hall. 960pp.

Bruehl, G.W. (1987) Soilborne Plant Pathogens. New York: Macmillan Publishing Company.368Pp.

Chehri, J. (2013). Factors affecting the growth of biomass of Aspergillus niger. Medical Sciences and Public Health, 1(1): 1-5.

Cobley, L. S., and Steele, W.M. (1976). An introduction to the Botany of Tropical Crops. London: Longman Groups Limited. 371Pp.

Cooke, R.C., and Whipps, J.M. (1993). Ecophysiology of Fungi.Oxford: Blackwell Scientific Publications. Pp. 1-348.

Cox, S. (2000). I say Tomyto, you say Tomahto. Available Online at: http://lamar.colostate.edu/~samcox/Tomato.html. Accessed on: 1/7/2017.

De Vries, P., Gelber, I.B., and Andersen, M.R. (2016). Aspergillus and Penicillium in the Post- Genomic Era. The Netherlands: Caister Academic Press. Pp.1-206.

Eurofresh Distribution (2016). Around the world: Tomatoes. Available Online at:http://www.eurofresh distribution.com/news/around-world-tomatoes Accessed on: 26/6/2017.

Feet, M. (2001). Sustainable Practices for vegetable Production in the South. Available Online at: http://wwwCalsNCSU.edu/sustenable/Feet/Profile/C19tom.html. Accessed on: 30/7/2017.

Harrison, J.G., Lowe, R., and Williams, N.A. (1994). Humidity and fungal diseases of plants – problems. In J.P. Blakeman and B. Williamson (Eds), Ecology of plant pathogens (Pp.79-97).Wallingford: CAB International.

Huber, L., and Gillespie, T.J. (1993). Modeling leaf wetness in relation to plant diseaseepidemiology. Annual Review of Phytopathology, 30: 553-577.

Ibrahim, M., Rabah, A.B., Liman, B., and Ibrahim, N.T. (2011). Effect of Temperature and Relative Humidity onthe Growth of Helminthosporiumfulvum. Nigerian Journal of Basic and Applied Science, 19(1):127-129.

Kim, K. Y., Jordan, D., and McDonald, G. A. (1998a). Effect of phosphate solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity. Biology and Fertility of Soils, 26(2): 79-87.

Koike, S.T., Cladders, P., and Paulus, A.O. (2007). Vegetable Diseases, A Color Handbook. Burlington, MA, USA: Academic Press. 448Pp.

Lifeofplant (2011). Plant Life: Deuteromycetes. Available Online at: http://lifeofplant.blogspot.com.ng/2011/04/deuteromycetes.html. Accessed on: 4/7/2017.

Muiz, D.E. (2012). Parasexual Cycle in Deuteromycota. Available Online at: http://www.uobabylon.edu.iq/uobcoleges/lecture.aspx?fid=5&lcid=31937. Accessed on: 1/7/2017.

Ncube, L., Nyari, Nyari, P., Mnkeni, S., and Brutsch, M.O. (2011). Agronomic suitability of effective micro-organisms for tomato production. African Journal of Agricultural Research, 6(3): 650-654.

Omolaran, B. B., Habib, U., Odunayo, O.J., Opeyemi. A.S., Alafe, A.H., and Temilade, O.A. (2016). Microorganisms causing post-harvest tomato (Solanum lycopersicum L.) fruit decay in Nigeria. Journal of Entomology and Zoology Studies, 4(1): 374-377.

Ososanya, J. (2018). Facts about Benue State and their languages. Available online at: https://www.legit.ng/1164560-facts-benue-state-languages.html. Accessed on: 22/06/2019.

Pandey, R.R., Arora, D.K., and Dubey, R.C. (1993). Antagonistic Interacterion Between Fungal Pathogens and Phylloplane Fungi of Guava. Mycopathology, 124: 31-39.

Raid, R. (2011). Plant Pathology Guidelines for Master Gardeners. University of Florida: Everglades Education and Research Centre. Available Online at: http://erec.ifas.ufl.edu/plant_pathology_guidelines/module_02.shtml. Accessed on: 26/6/2017.

Rick, C.M, Verna, J.W, Cherelat, R.T. (1990). Experimental Introgression to Cultivated tomato from related Wild Night Shades In A.B. Bennett and S.D.O. Neil (Eds), Horticulture Biotechnology. New York. Pp.19 – 32.

Published

08-04-2023

How to Cite

ISOLATION AND IDENTIFICATION OF FUNGI ASSOCIATED WITH SOILS AND DISEASED TOMATO PLANTS ON FARMERS’ FIELDS IN BENUE STATE NIGERIA. (2023). FUDMA JOURNAL OF SCIENCES, 3(2), 275-287. https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/1517

Issue

Vol. 3 No. 2 (2019): FUDMA Journal of Sciences - Vol. 3 No. 2

Section

Research Articles
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FUDMA Journal of Sciences

How to Cite

ISOLATION AND IDENTIFICATION OF FUNGI ASSOCIATED WITH SOILS AND DISEASED TOMATO PLANTS ON FARMERS’ FIELDS IN BENUE STATE NIGERIA. (2023). FUDMA JOURNAL OF SCIENCES, 3(2), 275-287. https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/1517

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