AQUEOUS WOOD-ASH EXTRACT OF PARKIA BIGLOBOSA IMPAIRED SPERMATOGENESIS IN MALE ALBINO MICE
Keywords:
Wood-ash extract, Reproductive Toxicity, Sperm Quality and Quantity, Seminiferous tubules, Reproductive HormonesAbstract
Wood-ash extracts are used as food additives and medicinals by different ethnic groups in Nigeria. Despite these uses, there is paucity of information on the possible adverse effects on the reproductive health in mammals. This study assessed the toxicity of aqueous wood-ash extract of Parkia biglobosa on reproductive organ in male albino mice. Four different dose levels of wood-ash extract of Parkia biglobosa [0 (control), 5, 50 and 100 mg/kg body weight] were orally administered to 20 mice (5 per group) for seven (7) consecutive days. After 35 days from first day of extract administration, blood samples were collected by retro-orbital phlebotomy and assayed for serum follicle stimulating hormone (FSH), Luteinizing hormone (LH) and testosterone. Mice were sacrificed subsequently by cervical dislocation and testes harvested to examine gonadosomatic index, sperm motility, sperm count, sperm morphology and testicular histopathology using standard methods. ANOVA was used to determine the significant difference among treatments, at P<0.05 level of significance. No significant effect was recorded in testicular weight, FSH, LH and testosterone levels; significant decrease in sperm motility, live/dead sperm and sperm count and increase of abnormal sperm cells were recorded. Frequencies of abnormal sperm cells were significantly higher at 100 mg/kg of the extracts than in control. Dosedependent depletion of spermatogenic cells were recorded in the testes of exposed mice. Aqueous wood ash extract of Parkia biglobosa could have damaging effects on sperms and testicular tissues, which can impair reproduction.
References
Afuwai, G. C. and Shadrach, H. S. (2020). Investigation of the Variation in Electrical Resistivity Values of Earth Materials with Depth at Narayi Area of Kaduna State, Nigeria. FUDMA Journal of Science, 4(1), 321 – 332.
Bhattacharyya, B.K. and Leu, L.K. (1977). Spectral analysis of gravity and magnetic anomalies due to rectangular prismatic bodies. Geophysics, 42, 41 – 50.
Chapman, D.S. and Furlong, K.P. (1987). The thermal state of lithosphere. Reviews of Geophysics, 25, 1255, 1264.
Chinwuko, A.I; Onwuemesi, A.G; Anakwuba, E.K. and Nwokeabia, N.C. (2012). Interpretation of aeromagnetic anomalies over parts of Upper Benue Trough and southern Chad Basin, Nigeria. Advances in Applied Science Research, 3(3) 1757 – 1766.
Dolmaz, M. N., Ustaomer, T. Hisarli, E. M. and Orbay, N. (2005). Curie point depth variations to infer structure of the crust at the African – Eurasian Convergence Zone, SW Turkey. Earth Planets Space, 57, 373 – 383.
Downing, R. A. and Gray, D. A. (1986). Geothermal energy - the potential in the United Kingdom. British Geological Survey report, 3 – 7.
Griscom, A. and Muffler, L. J. P. (1971). Aeromagnetic map and interpretation of the Salton Sea geothermal Area, California, US Geological Survey. Geophysical investigations report map GP –754.
Jessop, A. M., Hobart, M. A. and Schlater, J. G. (1976). The world heat flow data collection. Geothermal Services of Canada, 50: 55 – 77.
Komolafe, A. A. (2010). Investigations into the tectonic lineaments and thermal Structure of Kure, N., Aboh, O. H., Jimoh, R., Joseph, O. A., Daniel, I. H. (2017). The Delineation of Potential Groundwater Aquifers within Basement Complex in ABU Zaria, Nigeria. British Journal of Applied Science and Technology, 19(1), 1-9.
Kure, N., Daniel, I. H., Afuwai, C. G., Adoyi, E. J., Bello, I. A. (2019). The Delineation of Groundwater and Geotechnical Parameters within Marmara Area of Chikun Local Government of Kaduna State, Nigeria. Journal of the Nigerian Society of Physical Sciences, 1(2019), 1-5.
Spector, A. and Grant, F.S. (1970). Statistical models for interpreting aeromagnetic data. Geophysics, 35, 293 – 302.
Kurowska E, Krzysztof, S. (2010). Geothermal Exploration in Nigeria. Proceedings of the SWorld Geothermal Congress. Lake Madadi, southern Kenya rift using integrated geophysical methods.
Mariita, N. O. (2013). Applications of potential field methods for geothermal exploration – A case for Olkaria and Menengai geothermal fields, Kenya. Short Course VIII on Exploration for Geothermal Resources.
Nur, A., Ofoegbu, C. O. and Onuoha, K. M. (1999). Estimation of the depth to the Curie point isotherm in the Upper Benue Trough, Nigeria. Journal of Mining and Geology. 35 (1): 53 – 60.
Obande G.E. and Lawal A.A. (2013). Spectral analysis of aeromagnetic data for geothermal investigation of wiki warm spring, north-eastern Nigeria.
Onwuemesi, A.G. (1997). One-dimensional Spectral analysis of aeromagnetic anomalies and Curie depth isotherm in the Anambra Basin of Nigeria. J. Geodynamics, 23(2) 95 – 107.
Rybach, L. and Mulffler, L. J. P. (1981). Geothermal Systems: Principles and case histories. John Wiley and Sons, London.
Wright, J. B., Hastings, D. A., Jones, W. B. and Wiliams, H. R. (1985). Geology and mineral resources of West Africa. George Allen and Erwin, London. 90 – 120.
Published
How to Cite
Issue
Section
FUDMA Journal of Sciences
