EVALUATION OF THE PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITY OF Kalanchoe pinnata AGAINST PLANT PATHOGENS ISOLATED FROM DISEASED PLANT PATHOGENS
DOI:
https://doi.org/10.33003/fjs-2021-0503-755Keywords:
Kalanchoe pinnata, plant pathogen, biological control agentAbstract
The antimicrobial activity of Kalanchoe pinnata (Syn Bryophyllum pinnatum) against clinical pathogen is well documented in literature but there is paucity of information on its effect against plant pathogens. This work attempts to evaluate inhibitory activity of Kalanchoe pinnata (Syn Bryophyllum pinnatum) on selected plant pathogens. Aqueous, acetone, ethanol and methanol leaf, stem and root extracts of Kalanchoe pinnata (Syn Bryophyllum pinnatum) were prepared using standard techniques. Extracts were tested against bacteria and fungi isolated from some diseased plants, both singly and in combination with standard antimicrobials. Inhibitory activity was determined using agar well diffusion technique as well as broth dilution technique. Results indicate that the leaf ethanol extract was most effective against the plant pathogens. Zones of inhibition (mm) ranged from [19.0 ± 0.32] for Aspergillus flavus to [23.5 ± 0.22] for Xanthomonas campestris. Meanwhile, the Minimum inhibitory concentration (MIC) reduced from 6.25 mg/mL to 3.13 mg/mL (for Xanthomonas oryzae and Xanthomonas campestris) when leaf extract was used in combination with streptomycin. Furthermore, MIC reduced from 1.56 mg/mL to 0.78 mg/mL (for Aspergillus flavus and Aspergillus niger) when extract was used in combination with cycloheximide. Ethanol leaf extract of Kalanchoe pinnata (Syn Bryophyllum pinnatum) was most effective against selected plant pathogens. Also, effectiveness of extract was enhanced when used in combination with regular antimicrobial. Kalanchoe pinnata (Syn Bryophyllum pinnatum) may become useful as a biological control agent for plant disease pathogens
References
Alan, R., (2014). The effect of Nitrogen on Nutrition, Growth, Chemical composition and response of cucumbers to N forms in solution culture. Duke J (1997) The green pharmacy. St Martin’s Press, New York. J. Hort. Sci. 64:467-474.
Amulu L. U., and Adekunle O. K., (2015). Comparative Effects of Poultry Manure, Cow Dung, and Carbofuran on Yield of Meloidogyne incognita-Infested Okra. J. Agr. Sci. Tech. (2015) Vol. 17: 495-504.
Amulu L. U., and Adekunle O. K., (2015). Comparative Effects of Poultry Manure, Cow Dung, and Carbofuran on Yield of Meloidogyne incognita-Infested Okra. J. Agr. Sci. Tech. (2015) Vol. 17: 495-504.
Austin Deyo and Brendan O’maller., (2008). Food for Thought; the Science, Culture and Politics of Food (in Halies). College Seminar at Spring.
Barker K.R. 1985. Nematode extraction and bioassays. In: Barker KR, Carter CC, Sasser JN, editors. An advanced treatise on Meloidogyne. Vol. I, Methodology. Raleigh (NC): North Carolina State University Graphics; p. 19–35.
Bhattarai, B.P. and K.C. Prativa. (2011). Effect of Integrated Nutrient Management on the Growth, Yield and Soil Nutrient Status in Tomato. Nepal Journal of Science and Technolog. 12: 23-28.
Bin, L.; Sabine, R.; Guanlin, X.; Larsen, J., (2008). Differential effects of Paenibacillus spp. on cucumber mycorrhizas. Mycological Progress. (7): 277-284.
Bin, L.; Sabine, R.; Guanlin, X.; Larsen, J., (2008). Differential effects of Paenibacillus spp. on cucumber mycorrhizas. Mycological Progress. (7): 277-284.
Burney J.A., S.J. Davis, and D.B. Lobell, (2010). Greenhouse gas mitigation by agricultural intensification PNAS, 107 pp. 12052-1205.
Cheng, Y. F, Edwards, J. E, Allison, G. G, Zhu, W. Y and Theodorou, M. K., (2009). Diversity and activity of enriched ruminal cultures of anaerobic fungi and methanogens grown together on lignocellulose in consecutive batch culture. Bioresour Technol 100: 4821–4828.
Cheng, Y. F, Edwards, J. E, Allison, G. G, Zhu, W. Y and Theodorou, M. K., (2009). Diversity and activity of enriched ruminal cultures of anaerobic fungi and methanogens grown together on lignocellulose in consecutive batch culture. Bioresour Technol 100: 4821–4828.
Cordier, C.S. Gianinazzi and V. Gianinazzi-Pearson (1996). Colonisation patterns of root tissues by Phytophthora nicotiana var. parasitica related to disease in mycorrhizal tomato plant soil, 185:223–232
Eifediyi E. K. and Remison S. U., (2010). Growth and yield of cucumber (Cucumis sativus L.) as influenced by farmyard manure and inorganic fertilizer. Journal of Plant Breeding and Crop Science Vol. 2(7):216-220.
Hassan M. A., Chindo P. S., Marley P. S., and Alegbejo M. D., (2011). Management of Root Knot Nematodes (Meloidogyne spp.) on Tomato (Lycopersicon lycopersicum) Using Organic Wastes in Zaria, Nigeria. Plant Protect. Sci. 46, (1): 34–38.
Hause, B., Mrosk, C., Isayenkov, S., and Strack, D., (2007). Jasmonates in arbuscular mycorrhizal interactions. Phytochemistry, 68: 101-110.
Herrera-Medina, M. J., Steinkellner, S., Vierheilig, H., Ocampo Bote, J. A., & GarcÃa Garrido, J. M., (2007). Abscisic acid determines arbuscule development and functionality in the tomato arbuscular mycorrhiza. New Phytologist, 175: 554-564.
Lidan Zhang, Junling Zhang, Peter Christie and Xiaolin Li, (2008). Pre-inoculation with arbuscular mycorrhizal fungi suppresses root knot nematode (Meloidogyne incognita) on cucumber (Cucumis sativus). Biol Fertil Soils (2008) 45:205–211.
LInderman, R.G, (1994). Role of VAM fungi in bio–control: Mycorrhizal and plant health, Fleger, F.L and R.G Linderman (Eds). The American Phytopathological society, St. Paul, MN, USA. Pp 1–27.
Luc, M, Sikora, RA, Bridge, J. 2005. Plant parasitic nematodes in sub-tropical and tropical agriculture. Egham, UK: CABI Biosciences; p.45-115.
Noling J.W. (2009a). Nematode management in cucurbits (cucumber, melons, squash). ENY-025, Florida Cooperative Extension Service, University of Florida, Gainesville, FL. http://edis.ifas.ufl.edu/ng025.
Noling J.W. (2009b). Nematode management in tomatoes, pepper, and eggplant. ENY-032, Florida Cooperative Extension Service, University of Florida, Gainesville, FL. http://edis.ifas.ufl.edu/ng032.
Okonmah LU (2011). Effects of different types of staking and their cost-effectiveness on the growth, yield and yield components of cucumber (Cumumis sativa L). Int. J. of Agric. Sci. Vol. 1 (5): 290-295. International Academic Journals, Germany.
Nkechi B. IZUOGU, Oluwatoyin E. Bello and BELLO Oluwasesan M. (2018). Impact Assessment of Broiler Droppings and Root Grow (Mycorrhizal Fungi) on the Control of Meloidogyne Incognita Infecting Cucumbers (Cucumis Sativus L.) ’s Yield. FUDMA Journal of Agricultural and Agricultural Technology 4 (2), pp 103-112.
Oluwatoyin E. Bello., Bello O. M. and Dada A. O. (2013). Investigation of Aqueous extracts of Calotropis procera as a natural nematicide against root-knot Nematode infection on Abelmoschus esclentus L’s Yield. Inter. J. of Plant, Animal and Environmental Science. Vol 3(4), pp 175-177.
Oluwatoyin E. Bello., Bello O. M. and Dada A. O. (2013b). The Nematicidal effect of Aqueous extracts of Leaves of Calotropis procera against Root-Knot Nematode infection on Vegetative Growth of Okra Plants. Inter. J. of Plant, Animal and Environmental Science. Vol 3 (4), pp 193-196.
Nkechi B. Izuogu, Oluwatoyin E. Bello and Bello Oluwasesan M. (2020). A Review on Borreria verticillata: from Weed to Sustainable Agriculture. Heliyon. 6: e05322, doi.org/10.1016/j.heliyon.2020.e05322
Singh Satyandra. 2013. An integrated approach for the management of root-knot nematode, Meloidogyne incognita on eggplant under field conditions. Nematology 32:1-9.
Vermis J. C., Roberts P. A. (1996). Relationships between Meloidogyne incognita resistance gene in Lycopersicon peruvianum differentiated by heat sensitivity and nematode virulence. Theor Appl Genet. 93:950–959.
Vimala P, Ting CC, Salbiah H, Ibrahim B, Ismail L (2009). Biomass production and nutrient yields of four green manures and their effects on the yield of cucumber. Journal of Tropical Agric and Food Science 27:47-55.
Zasada, I. A., Halbrendt, J. M., Kokalis-Burelle, N., LaMondia, J., McKenry, M. V., and Noling, J. W., (2010). Managing nematodes without methyl bromide. Annual Review of Phytopathology 48:311–328.
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