PHYTOCHEMICAL, ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF THE CRUDE EXTRACT OF Curvulariasp. ISOLATED FROM THE LEAVES OF THE MEDICINAL PLANT Piliostigma thonningii (Fabaceae)

  • Cyril Ogbiko Madonna University Elele Rivers State
  • C. J. Eboka
  • U. M. Okezie
  • F. B. C. Okoye
Keywords: Antioxidant, Antimicrobial, Curvulariasp, Phytochemical, Secondary Metabolites

Abstract

Recently, there is biotechnological interest in secondary metabolites of endophytes since they have been shown to be capable of producing substances of pharmacological importance. Piliostigmathonningii is a tree whose parts are widely employed in Nigeria for diverse medicinal applications. The aim of this study is to investigate the phytochemical composition, the antiradical and in vitro antimicrobial potentials of the crude ethyl acetate extract of Curvularia sp isolated from the leaves of the plant. The endophytic fungus was isolated, purified, and identified using conventional methods. While standard procedures was employed in the determination of the phytochemical constituents, quantitative estimation of the total phenol and flavonoid were done by spectrophotometric method using gallic acid and quercetin as standards respectively. While the antioxidant activity was determined by DPPH scavenging assay, the agar well diffusion method was employed to screen for its antimicrobial capabilities. Results showed the presence ofimportant phytoconstituents notably alkaloid, flavonoid, phenolics, tannin, saponins among others. While the antioxidant assay showed the total phenolic and flavonoid contents to be 212.55 ± 1.2 mgGAE/g extract and 143.16 ± 0.8 mgQE/g extract respectively, a 74.45 ± 0.11% free radical scavenging activity was observed compared to ascorbic acid standard that exhibited a 87.92 ± 0.17% inhibition. At the highest investigated concentration of 1 mg/mL, the extract produced antibacterial activity only against Escherichia coliandPseudomonas aeruginosa. The results showed that the extract exhibits marked antioxidant and selected antibacterial effects thus justifying the assertion that endophytic bioactive compounds have promising applications in the field of medicine.

References

Akinpelu, A.D., Awotorebo, T.O., Agunbiade, O.M., Ayyegoro, A.O. and Okoh, I.A. (2011).Anti-vibrio and preliminary phytochemical characteristics of crude methanolic extracts of the leaves of Dialium guineense (Wild), Journal of Medicinal Plant Research, 5(11), 2398-2404.

Akpotu, M.O., Eze, P.M., Abba, C.C., Nwachukwu, C.U., Okoye, F.B.C. and Esimone, C.O. (2017)a. Metabolites of endophytic fungi Isolated from Euphorbia hirtagrowing in Southern Nigeria, Chemical Science Review and Letters, 6(21): 12 – 19.

Akpotu, M.O., Eze, P.M., Abba, C.C., Umeokoli, B.O., Nwachukwu, C.U., Okoye, F.B.C., Esimone, C.O. (2017)b. Antimicrobial activities of secondary metabolites of endophytic fungi isolated from Catharanthus roseus, Journal of Health Science, 7(1): 15-22.

Chika, C.A., Peter M.E., Dominic, O.A., Charles, U.N., Peter, P., Okoye, F.B.C. and Eboka, C.J. (2018).Phenolic Compounds from Endophytic Pseudofusicoccumsp. Isolated from Annona muricata. Tropical Journal of Natural Product Research, 2(7):332-337.

Cook, N.C. and Samman, S. (1996). Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. Nutri Biochem, 7: 66-76.

Daniyan, S.Y. and Abalaka, M.E. (2012).Antimicrobial activity of stem extracts of Piliostigma thonningii, Journal of Science and Multidisciplinary Research, 1(2), 8-13.

Daniyan, S.Y., Galadima, M., Ijah, U.J.J., Odama, L.E., Yusuf, A.Y. and Abbas, Y. (2010).In vitro antibacterial screening of Piliostigma thonningii (Schum) Milne Red-Head leaves extracts against clinical isolates of methicillin resistant and methicillin sensitive Staphylococcus aureus, International Journal of Pure and Applied Sciences, 4(1), 88-94.

Deepa, M. and Renuka, D. (2014). Potent anti-inflammatory medical plant-a review, Int J Pharm Pharm Sci., 6(4):43-49.

Ebrahimzadeh, M.A., Pourmorad, F., Bekhradnia, A.R. (2008).Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran, Afr. J. Biotech, 7, 3188-3192.

Evans, W.C. (2002). Trease and Evans Pharmacognosy.Elsiever India, 15th edition. 27(46), 183-184.

Ezeja, M.I., Yusuf, N., Omeh, S., Onoja, O. and Ijeoma, H.U. (2015). Antiinflammatory and antioxidant activities of the methanolic leaf extract of Cissus aralioides, Am J Pharmacol Sci, 3 (1): 1-6.

Farrukh, A., Iqbal, A. and Zafar, M. (2006). Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turk. J. Biol, 30: 177-183.

Gandía-Herrero, F., Escribano, J. and García-Carmona, F. (2009).The role of phenolic hydroxy groups in the free radical scavenging activity of betalains, Journal of Natural Products, 72, 1142–1146.

Harman, D. (1981). The aging process. Proc, Natl Acad. Sci, USA. 78: 7124-7128

Jain, A., Soni, M., Deb, L., Jain, A., Rout, S., Gupta, V. and Krishna, K. (2008). Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica leaves, J. Ethnopharmacol, 115, 61-66.

Khandewal, K.R. (2000). Practical Pharmacognosy Technique and Experiments, 9th edn,Nirali Publications Pune, pp. 149-156.

Kim, D.O., Jeong, S.W. and Lee, C.Y. (2003).Antioxidant capacity of phenolic phytochemicals from various cultivars of plums, Food Chem, 81, 321-326.

Kolodziej, H. and Kiderlen, A.F. (2005).Antileishmanial activity and immune modulatory effects of tannins and related compounds on Leishmania parasitised RAW 264.7 cells, Journal of Phytochemistry, 66(17), 2056-2071.

Kumpulainen, J.T. and Salonen, J.T. (1999). Natural Antioxidants and Anticarcinogens in Nutrition, Health and Disease, The Royal Society of Chemistry, UK. pp 178-187.

Kwaji, A., Bassi, P.U., Aoill, M., Nneji, C.M. and Ademowo, G. (2010).Preliminary studies on PiliostigmathonningiiSchumleaf extract: Phytochemical screening and in vitro antimalarial activity. African Journal of Microbiology Research, 4(9): 735-739.


Maisuthisakul, P., Pasuk, S. and Ritthiruangdej, P. (2008).Relationship between antioxidant properties and chemical composition of some Thai plants, Journal of Food Composition and Analysis, 21(3), 229-240.

Maisuthisakul, P., Pongsawatmanit, R. and Gordon, M.H. (2007).Assessment of phenolic content and free-radical scavenging capacity of some Thai indigenous plants, Food Chemistry, 100, 1409-1418.

Ogbiko, C., Eboka, C.J. and Tambuwal, A.D. (2018).Extraction and fractionation of whole black seed plantain (Plantago rugelii Decne) for in-vitro antioxidant, antibacterial and phytochemical screening, Journal of Applied Sciences and Environmental Management, 22(5), 613– 618.

Ogbiko, C., Okoye, F.B.C. and Eboka C.J. (2020).Phytochemical, in vitro antimicrobial, antioxidant properties and polyphenolic content of methanol leaves extract of Piliostigma thonningii Schum Milne-Redhead (Fabaceae). African Journal of Pharmaceutical Research and Development. 12(3): 299 – 312.

Okoye, F.B.C., Nworu, C.S., Debbab, A., Esimone, C.O. and Proksch, P. (2015).Two new Cytochalasins from an endophytic fungus, KL-1.1 isolated from Psidium guajava leaves. Phytochem Lett, 14:51-55.

Okoye, F. B. C. and Osadebe, P. O.(2010). 'A new anti-inflammatory flavonol glycoside from Alchornea floribunda leaves', Natural Product Research, 24(3): 266 – 273.

Vijay, P. and Vimukta, S. (2014). The role of natural antioxidants in oxidative stress induced diabetes mellitus, Research Journal of Pharmaceutical Sciences, 3, 1-6.

Yadav, M., Yadav, A. and Yadav, J.P. (2014).In vitro antioxidant activity and total phenolic content of endophytic fungi isolated from Eugenia jambolana Lam, Asian Pacific Journal of Tropical Biomedicine, 7(suppl. 1): 5256 – 5261.

Zhang, L. and Lin, Y. (2008).Tannins from Canarium album with potent antioxidant activity, Journal of Zhejiang University Science, 9(5), 407-415.

Zhang, Y.J., Gan, R.Y., Li, S., Zhou, Y., Li, A.N. and Xu, D.P. (2015). Antioxidant phytochemicals for the prevention and treatment of chronic diseases, Molecules, 20(12): 21138–21156.
Published
2021-11-03
How to Cite
Ogbiko, C., Eboka, C. J., Okezie, U. M., & Okoye, F. B. C. (2021). PHYTOCHEMICAL, ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF THE CRUDE EXTRACT OF Curvulariasp. ISOLATED FROM THE LEAVES OF THE MEDICINAL PLANT Piliostigma thonningii (Fabaceae). FUDMA JOURNAL OF SCIENCES, 5(3), 281 - 285. https://doi.org/10.33003/fjs-2021-0503-687