OPTIMIZATION OF LACCASE PRODUCTION BY CURVULARIA LUNATA USING MAIZE COB AS SUBSTRATE
Researchers are showing interest in laccase because it is able to degrade several lignocellulosic biomass. It is important to optimize laccase production to achieve higher yield at a lower cost using agricultural wastes. This study was aimed at optimizing the culture conditions for laccase production. Previously isolated and characterized laccase producing Curvularia lunata was collected from the laboratory of Microbiology department, ABU, Zaria. Maize cobs which were used as substrate were collected from Seed Processing Unit of Institute for Agricultural Research, Zaria and the proximate composition of the substrate was determined according to AOAC guideline. The optimum fermentation type for laccase production was determined after which the culture conditions for laccase production were optimized. Laccase activity was determination by guaiacol assay. The proximate compositions of the maize cob were found to be Moisture content (3.18%), Crude protein (2.50%), Crude fat (32.20%), Crude fibre (3.15), Ash content (1.88%) and Carbohydrate content (57.09%). Higher laccase activity was recorded under solid state fermentation compared to submerged fermentation. The optimum culture conditions were found to be inoculum size 3 x 5 mm, pH 5, incubation temperature 30 oC and incubation period of 6 days. The culture conditions for laccase production by Curvularia lunata using maize cob as substrate were optimized
Abd El Monssef, R.A., Hassan, E.A. and Ramadan, E.A. (2016). Production of laccase enzyme for their potential application to decolorize fungal pigments on aging paper and parchment. Annals of Agricultural Science, 61(1):145-154.
Abdulredha, S.S. (2013). Production of laccase from Pleurotus spp. by Solid State Fermentation using agricultural wastes A Thesis submitted to the College of Science as a partial fulfillment of the requirements for the degree of M.Sc in Biotechnology. College of Science, University of Baghdad.
Amutha, C. and Abhijit, M. (2015). Screening and Isolation of Laccase Producers, Determination of Optimal Condition for Growth, Laccase Production and Choose the Best Strain. Journal of Bioremediation and Biodegradation, 6(4):1-8.
Amutha, C., Subramanian, P. and Manna, A. (2014). Bio-remediation its basic modern methods application a review. International Journal of Trends in Applied Microbiology and Biotechnology, 3:49-76.
AOAC (2010). Official Methods of Analysis of the Association of Analytical Chemists, (18th edition). Washington, D.C. Association of Analytical Chemists.
Bhuvaneshwari, V., Preethikaharshini, J., Amsaveni, R. and Kalaiselvi, M. (2015). Isolation, optimization and production of laccase from Halobacillus halophilus. International Journal of Biosciences and Nanosciences, 2(2):41-47.
Brijwani, K., Rigdon, A. and Vadlani, P.V. (2010). Fungal Laccases Production, Function and Applications in Food Processing. Enzyme Research, 2010(149748):1-10.
Buddolla, V., Chandra, M.S. Pallavi, H. and Reddy, B.R. (2008). Screening and assessment of laccase producing fungi isolated from different environmental samples. African Journal of Biotechnology, 7(8):1129-1133.
Chan, M.Y., Goh, S.M., Gaik, L. and Ong, A. (2016). Isolation and Screening of Laccase Producing Basidiomycetes via Submerged Fermentations. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering, 10(2):77-80.
Couto, S.R. and Herrera, J.L.T. (2006). Industrial and biotechnological applications of laccases: a review. Biotechnology Advances, 24(5):500–513.
Das, N., Dey, D. and Mishra, S. (2015). Isolation and physico-chemical characterization of extracellular lingo-cellulolytic enzymes of Pleurotus pulmonarius in submerged fermentation. International Journal of Applied Biology and Pharmaceutical Technology 6(3), 15-23.
Desai, S.S., Tennali, G.B., Channur, N., Anup, A.C., Deshpande, G. and Azhar Murtuza, B.P. (2011). Isolation of laccase producing fungi and partial characterization of laccase. Biotechnology, Bioinformatics and Bioengineering, 1(4):543-549.
Dos Santos Bazanella, G.C., De Souza, D.F. and Castoldi, R. (2013). Production of laccase and manganese peroxidase by Pleurotus pulmonarius in solid state cultures and application in dye decolorization. Folia Microbiology, 58:641-647.
Faradi de Souza, D., Claudio da Costa, S., Dacome, A.S., Marquez de Souza, C.G., Bracht, A. and Peralta, R.M. (2011). Pentachloro removal by Pleurotus pulmonarius in submerged cultures. Brazilian Archive of Biology/ Technology, 54(2):355-362.
Giardina, P., Faraco, V., Pezzella, C., Piscitelli, A., Vanhulle, S. and Sannia, G. (2010). Laccases: a never-ending story. Cellular and Molecular Life Science, 67:369-385.
Gomaa, E.Z. (2013). Some applications of α-amylase produced by Bacillus subtilis NCTC-10400 and Bacillus cereus ATCC 14579 under solid state fermentation. African Journal of Microbiology Research, 7(29):3720-3729
Hao, J., Song, F., Huang, F., Yang, C., Zhang, Z., Zheng, Y. and Tian, X. (2007). Production of laccase by a newly isolated deuteromycete fungus Pestalotiopsis sp. and its decolorization of azo dye. Journal Industry Microbiology and Biotechnology, 34:233-240.
Herrera, J.L.T., Osma, J.F. and Couto, S.R. (2007). Potential of Solid-State Fermentation for Laccase Production. Communicating Current Research and Educational Topics and Trends in Applied Microbiology.
Hong, L.S., Ibrahim, D. and Omar, C. (2011). Lignocellulolytic materials as raw materials for the production of fermentable sugars. Asian Scientific Research, 4(1):53-61.
Kalra, K., Chauhan, R., Shavez, M., Sachdeva, S. (2013). Isolation of laccase producing Trichoderma spp. and effect of pH and temperature on its activity. International Journal of Chemistry and Environmental Technology, 5(5):2229-2235.
Kammoun, M.M., Mechichi, H.Z., Belbahri, L., Woodward, S. and Mechichi, T. (2009). Malachite green decolurization and detoxification by the laccase from a newly isolated strain of Trametes sp. International Biodeterioration and. Biodegradation, 63:600-606.
Karpouzas, D.G. and Singh, B.K. (2006). Microbial degradation of organo-phosphorus xenobiotics metabolic pathways and molecular basis. Advances in Microbial Physiology, 51:119-185.
Masutti, D., Borgognone, A. and Setti, L. (2012). Production of enzyme from rice husks and wheat straw in solid state fermentation. Chemical Engineering Trends, (27):133-138.
Mayera, A.M. and Staples, R.C. (2002) Laccase new functions for an old enzyme. Phytochemistry 60: 551-565.
Megersa, S., Gure, A., Alemu, M. and Feleke, S. (2017). Qualitative Assays and Quantitative Determinations of Laccases of White Rot Fungi from Plantation and Natural Forests of Arsi Forest Enterprise, Ethiopia. World Scientific News, 67(2):303-323.
Mtui, G.Y.S. (2012). Lignocellulolytic enzymes from tropical fungi: Types, substrates and applications. Scientific Research and Essays, 7(15):1544-1555.
Niladevi, K.N., Sukumaran, R.K. and Prema, P. (2007). Utilization of Rice Straw for Laccase Production by Streptomyces psammoticus in Solid-State Fermentation. Journal of Industrial Microbiology and Biotechnology, 34:665-674.
Patel, H., Gupte, A. and Gupte, S. (2009). Effect of different culture conditions and inducers on production of laccase by a Basidiomycete fungal isolated Pleurotus ostreatus HP-1 under solid state fermentation. Bioresources, 4(1):268-284.
Pointing, S.B. Jones, E.B.G. and Vrijmoed, L.L.P. (2001). Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture, Mycologia., 92(1):139-144.
Risdianto, H., Sofianti, E., Suhardi, S.H. and Setiadi, T. (2012). Optimisation of Laccase Production using White Rot Fungi and Agriculture Wastes in Solid State Fermentation. ITB Journal of Engineering Science, 44(2):93-105.
Sahay, R., Yadav, R.S.S. and Yadav, K.D.S. (2009). Purification and Characterization of Laccase Secreted by L. lividus. Applied Biochemistry and Biotechnology, 157:311-320.
Saqib, H.H., Muhammad, J.A., Muhammad, G., Muhammad, A., Nasir, M.M., Raja T.M., Shehnaz, Z. and Nasir, M. (2015). Solid State Fermentation for the production of Laccase by Neurospora sitophila using agro-wastes and its partial purification. International Journal of Biochemistry and Biotechnology, 4(5):564-573.
Shraddha, S.R., Sehgal, S., Kamthania, M. and Kumar, A. (2011). Laccase: microbial sources, production, purification, and potential biotechnological applications, Enzyme Research, 2011:1-11.
Sidhu, A.K., Darade, S.B., Bhavsar, P.P., Gaikwad, V.B. and Patil, S.N. (2017). Isolation, Screening and Optimization for Laccase production by Scytalidium lignicola pesante under submerged fermentation. International Journal Current Microbiology and Applied Science, 6(4):2477-2491.
Singleton, I. (1994) Microbial metabolism of xenobiotics. Fundamental and applied research. Journal of Chemical Technology and Biotechnology, 59: 9-23.
Sinha, S., Chattopadhyay, P., Pan, I., Chatterjee, S. and Chanda, P. (2009). Microbial transformation of xenobiotics for environmental bioremediation. African Journal of Biotechnology, 8:6016-6027.
Szabo, O.E., Csiszar, E., Toth, K., Szakacs, G. and Koczka, B. (2015). Ultrasound-assisted extraction and characterization of hydrolytic and oxidative enzymes produced by solid state fermentation. Ultrasonic Sonochemistry, 22:249-256.
Vantamuri, A.B. and Kaliwal, B.B. (2015). Isolation, Screening and Identification of Laccase Producing Fungi. International Journal of Pharmaceutical and Biological Sciences, 6(3):242 - 250
Whiteley, C.G. and Lee, D.-J. (2006). Enzyme technology and biological remediation. Enzyme and Microbial Technology, 38:291-316.
Xu, X., Feng, L., Han, Z., Luo, S., Wu, A., and Xie, J. (2016). Selection of high laccase-producing Coriolopsis gallica strain T906: mutation breeding, strain characterization, and features of the extracellular laccases. Journal of Microbiology and Biotechnology, 26:1570-1578.
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