BIOFILM ERADICATION ACTIVITY OF PARTIALLY PURIFIED FRACTION OF ETHYLACETATE EXTRACT OF ACALYPHA WILKESIANA LEAVES ON CANDIDA ALBICANS I, II AND PARAPSILOSIS
Candida spp produce highly structured biofilms composed of multiple cells encased in an extracellular matrix. Sessile cells are known to decrease drug diffusion toward biological targets. This research is aimed at evaluating the biofilm eradication activity of partially purified fraction of ethylacetate extract of Acalypha wilkesiana leaves on sessile cells of C. albicans I, II and C. parapsilosis. Column Chromatography-TLC was used to fractionate and partially purify the extract using a bioassay guided fractionation. Preparative TLC was used to purify the most potent fraction. Antifungal effects of the extract and percentage biofilm eradication was determined by microdilution method. Antibiofilm activity of the purified fraction on the morphology of the sessile cells of Candida spp was evaluated by scanning electron microscopy. The purified fraction had MIC of 1.25mg/ml on C. albicans I, II and C. parapsilosis, respectively. MFC of 2.5mg/ml on C. albicans I and II but there was no total death observed on C. parapsilosis. The fraction had IC50 significantly (p ≤ 0.05) different from that of the reference drugs. There was significant (p ≤ 0.05) increase in the biofilm eradicated by corilagin, amp B., voriconazole, caspafungin, compared to the purified fraction on C. albicans I, II and C. parapsilosis. There was decrease in biomass of sessile cells on the morphology of C. albicans I, II and C. parapsilosis in all treated plates compared to the control. Therefore, the purified fraction of ethylacetate extract of A. wilkesiana leaves has a potential to eradicate the biofilms formed by Candida spp.
Douglas RobertoMonteiro1, Leonardo PerinaFeresin1, La ́ıs Salom ̃aoArias1, Valentim Adelino Ricardo,Debora BarrosBarbosa3and Alberto Carlos BotazzoDelbem (2015). Effect of Tyrosol on Adhesion of Candida albicans and Candida glabrata to acrylic surfacesMedical Mycocology. 53: 656–66.
Donlan RM (2002). Biofilms: Microbial Life on Surfaces. Emerg Infect Dis. 8:881-890.
Fox E.P., and Nobile C.J. (2012). A Sticky Situation: Untangling the Transcriptional Network Controlling Biofilm Development in Candida albicans. Transcription. 3:315–22.
Guilherme R Teodoro, Fernanda L Brighenti, Alberto C Botazzo , Delbem Ádina ,Cléia B Delbem , Sonia Khouri Aline, Vidal L Gontijo , Aislan CRF Pascoal , Marcos J Salvador, Cristiane Y Koga-Ito ( 2015 ). Antifungal activity of extracts and isolated compounds from Buchenavia tomentosa on Candida albicans and non-albicans.Future Microbiology. 10 (6) 1120-1124
Handa SS, Khanuja SPS, Longo G, Rakesh DD (2008). Extraction Technologies for Medicinal and Aromatic Plants, (1stedition). 66.
Javier Peman, Miguel Salavert, Emilia Canton, Isidro Jarque,Eva Roma, Rafael zAragoza, AngelViudes and Miguel Gobernado (2006). Voriconazole in the Management of Nocosomial Invasive Fungal Infections. Therapeutics and Clinical Risk Management. 2(2) :129-158.
Jessica A. Edwards, Megan M. Kemski, Chad A. Raphele (2013). Identification of an Aminothiazole with Antifungal Activity Against Intracellular Histoplasma Capsulation. Antimicrobial Agents and Chemotherapy. 57: 49-59.
Kojic E.M., Darouiche RO (2004). Candida infections of Medical Devices. Clin Microbiol Rev. 17:255-267.
Kruppa, M. (2009). Quorum Sensing and Candida albicans. Mycoses: 52: 1–10.
Muyideen T. Haruna, Chinedu P. Anokwuru, Abosede A. Akeredolu, Adenike A. Akinsemolu and Okunola A. Alabi (2013). Antibacterial and Antifungal Activity of Acalypha wilkesiana. European Journal of Medicinal Plant. 3(1): 52-64.
Nobile C.J.and Mitchell AP. (2005). Regulation of Cell-surface Genes and Biofilm Formation by the C. albicans transcription factor Bcr1p. Curr Biol. 15:1150–1155.
Premanath R and Lakshmideri N. (2010). J American Sci. 6 (10):736-743.
Ramage G., Martinez J.P., Lopez-Ribot J.L. (2006). Candida Biofilms on Implanted Biomaterials: A Clinically Significant Problem. FEMS Yeast Res. 6:979-986.
Ramage G., Mowat E., Jones B., Williams C., Lopez-Ribot J (2009). Our Current Understanding of Fungal Biofilms. Crit Rev Microbiol. 35:340-355.
Russell, A. D., Furr J. R. (1977). The Antibacterial Activity of a New Chloroxylenol Preparation Containing Ethylene Diamino Tetracetic Acid. Journal of Applied Bacteriology. 43: 253-255.
Silva S, Negri M, Henriques M. (2011). Adherence and Biofilm Formation of non-Candida albicans Candida Species. Trends Microbiol. 19(5): 241–247.
Siddiqui, Z. N., Farooq, F., Musthafa, T. N. M., Ahmad, A., and Khan, A. U. (2013). Synthesis: Characterization and Antimicrobial Evaluation of Novel Halopyrazole Derivatives. Journal of Saudi Chemical Society. 17: 237–243.
Takahagi Nakaira E., Sugizaki MF, Percoli MTS (2009). Microdilution Procedure for Antifungal Susceptibility Testing of P. brasilienis to Amphotericin B. and Itraconazole. J. Venom Animal Toxins including Tropical Diseases. Vol. 15. P 718-731.
Copyright (c) 2020 FUDMA JOURNAL OF SCIENCES
This work is licensed under a Creative Commons Attribution 4.0 International License.
FUDMA Journal of Sciences