EXTRACTION OF ANTIMICROBIAL COMPOUNDS FROM PSIDIUM GUAJAVA LEAVES AND THEIR APPLICATION IN SOAP FORMULATION WITH AZADIRACHTA INDICA SEED OIL

Authors

  • Ismail Muhammad
    Ibrahim Shehu Shema Center for Renewable Energy Research, Umaru Musa Yar'adua University, Katsina,Umaru Musa Yar'adua University, Katsina
  • Ahmed Lawal Mashi
    Umaru Musa Yar'adua University image/svg+xml

Keywords:

Psidium guajava, Azadirachta indica, Soap formulation, FTIR characterization, Antimicrobial activity

Abstract

The search for sustainable antimicrobial agents has led to increasing interest in bioactive compounds from medicinal plants. This study investigated the extraction of antimicrobial compounds from Psidium guajava leaves and their application in soap formulation with Azadirachta indica (neem) seed oil. Phytochemical screening revealed the presence of phenols, tannins, flavonoids, terpenoids, glycosides, and saponins. Maceration of 50 g of guava leaves yielded 10.68 g of methanolic extract compared to 9.65 g with ethanol, indicating higher efficiency of methanol extraction. FTIR spectra of guava leaf extracts identified functional groups such as O–H stretching (3250.20 cm⁻¹), C=O stretching (1689.30 cm⁻¹), and aromatic C=C stretching (1602.80 cm⁻¹), while neem seed oil exhibited C=C unsaturation (1654.90 cm⁻¹) and O–H stretching (3369.50 cm⁻¹), confirming the presence of phenolic compounds, flavonoids, and unsaturated fatty acids. Performance analysis of guava leaf soap (GLS) showed formability of 10.70 cm, pH of 10.80, and hardness index (needle penetration) of 3.10 cm, comparable to commercial Dudu Osun soap (formability 11.10 cm, pH 9.45, hardness 2.90 cm). Antimicrobial assays demonstrated that GLS inhibited Staphylococcus aureus (19.00 mm), Bacillus aureus (17.50 mm), Aspergillus niger (18.00 mm), and Candida albicans (18.00 mm) at 500 mg/mL, with inhibition zones statistically comparable to those of Dudu Osun (18.00–20.50 mm). These findings confirm that guava leaf extracts and neem oil can be effectively harnessed for antiseptic soap production, providing a natural, cost-effective alternative to synthetic formulations with demonstrated antibacterial and antifungal activity.

Dimensions

Ali, H., Abubakar, M., & Bello, A. (2020). Production and evaluation of herbal antiseptic soaps using neem (Azadirachta indica) oil. Nigerian Journal of Chemical Research, 25(2), 45–53. https://doi.org/10.4314/njcr.v25i2.4

Ananthapadmanabhan, K. P., Moore, D. J., Subramanyan, K., Misra, M., & Meyer, F. (2004). Cleansing without compromise: the impact of cleansers on the skin barrier and the technology of mild cleansing. Dermatologic Therapy, 17(1), 16–25. https://doi.org/10.1111/j.1396-0296.2004.04002.x

AOAC. (2005). Official methods of analysis of AOAC International (18th ed.). Association of Official Analytical Chemists.

Arima, H., & Danno, G. (2002). Isolation of antimicrobial compounds from guava (Psidium guajava L.) and their structural elucidation. Bioscience, Biotechnology, and Biochemistry, 66(8), 1727–1730. https://doi.org/10.1271/bbb.66.1727

Barbalho, S. M., Farinazzi-Machado, F. M. V., de Alvares Goulart, R., Brunnati, A. C., Ottoboni, A. M. M. B., & Nicolau, C. C. T. (2012). Psidium guajava (guava): a plant of multipurpose medicinal applications. Medicina, 45(5), 420–429. https://doi.org/10.11606/issn.2176-7262.v45i5p420-429

Biswas, K., Chattopadhyay, I., Banerjee, R. K., & Bandyopadhyay, U. (2002). Biological activities and medicinal properties of neem (Azadirachta indica). Current Science, 82(11), 1336–1345. https://www.jstor.org/stable/24107241

Clinical and Laboratory Standards Institute (CLSI). (2012). Performance standards for antimicrobial susceptibility testing (22nd ed., CLSI document M100-S22). Wayne, PA: CLSI.

Coates, J. (2000). Interpretation of infrared spectra, a practical approach. In Meyers, R. A. (Ed.), Encyclopedia of Analytical Chemistry (pp. 10815–10837). Wiley. https://doi.org/10.1002/9780470027318.a5606

Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582. https://doi.org/10.1128/CMR.12.4.564

Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S., & Ju, Y. H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3), 296–302. https://doi.org/10.1016/j.jfda.2013.11.001

Gutiérrez, R. M. P., Mitchell, S., & Solis, R. V. (2008). Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology, 117(1), 1–27. https://doi.org/10.1016/j.jep.2008.01.025

Harborne, J. B. (1998). Phytochemical methods: A guide to modern techniques of plant analysis (3rd ed.). Chapman & Hall.

Hernández-Carlos, B., & Gamboa-Angulo, M. M. (2011). Metabolites from Psidium guajava L. with potential antifungal properties. Physiological and Molecular Plant Pathology, 79(1), 1–9. https://doi.org/10.1016/j.pmpp.2012.04.001

Khokhar, S., & Owusu Apenten, R. K. (2003). Iron binding characteristics of phenolic compounds: some tentative structure–activity relations. Food Chemistry, 81(1), 133–140. https://doi.org/10.1016/S0308-8146(02)00394-1

Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 2013, 162750. https://doi.org/10.1155/2013/162750

Kumar, S., Pandey, A. K. (2015). Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 2013, 162750. https://doi.org/10.1155/2013/162750

Nielson, S. S. (2010). Food analysis laboratory manual (2nd ed.). Springer. https://doi.org/10.1007/978-1-4419-1463-7

Ojewole, J. A. O. (2006). Antiinflammatory and analgesic effects of Psidium guajava Linn. (Myrtaceae) leaf aqueous extract in rats and mice. Methods and Findings in Experimental and Clinical Pharmacology, 28(7), 441–446. https://doi.org/10.1358/mf.2006.28.7.986492

Okoroafor, U. J., Ekpo, D. E., & Udoh, A. P. (2014). Production and quality evaluation of soap from neem oil and shea butter. International Journal of Applied Research and Technology, 3(5), 89–96.

Okoye, C. O. B., Agbo, C. U., & Anaduaka, E. G. (2017). Physicochemical properties and antimicrobial activities of herbal soaps formulated with plant extracts. Journal of Pharmaceutical Research International, 18(6), 1–11. https://doi.org/10.9734/JPRI/2017/37582

Okumu, M. O., Mburu, N. D., Mbaria, J. M., & Gakuya, D. W. (2018). Medicinal properties of neem (Azadirachta indica) in veterinary medicine: a review. International Journal of Veterinary Science and Medicine, 6(1), 11–16. https://doi.org/10.1016/j.ijvsm.2018.03.001

Oyeniran, K. A., Lawal, A. O., & Oyebanji, M. A. (2020). Production and quality evaluation of antiseptic soap from neem oil and guava leaves extract. Journal of Applied Sciences and Environmental Management, 24(5), 903–908. https://doi.org/10.4314/jasem.v24i5.24

Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., & Latha, L. Y. (2011). Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African Journal of Traditional, Complementary and Alternative Medicines, 8(1), 1–10. https://doi.org/10.4314/ajtcam.v8i1.60483

Sofowora, A. (1993). Medicinal plants and traditional medicine in Africa (2nd ed.). Spectrum Books.

Thompson, S. K. (2012). Sampling (3rd ed.). Wiley.

Trease, G. E., & Evans, W. C. (2009). Pharmacognosy (16th ed.). Saunders Elsevier.

World Health Organization (WHO). (2020). Antimicrobial resistance. https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance

Published

28-09-2025

How to Cite

EXTRACTION OF ANTIMICROBIAL COMPOUNDS FROM PSIDIUM GUAJAVA LEAVES AND THEIR APPLICATION IN SOAP FORMULATION WITH AZADIRACHTA INDICA SEED OIL. (2025). FUDMA JOURNAL OF SCIENCES, 9(10), 12-18. https://doi.org/10.33003/fjs-2025-0910-3957

How to Cite

EXTRACTION OF ANTIMICROBIAL COMPOUNDS FROM PSIDIUM GUAJAVA LEAVES AND THEIR APPLICATION IN SOAP FORMULATION WITH AZADIRACHTA INDICA SEED OIL. (2025). FUDMA JOURNAL OF SCIENCES, 9(10), 12-18. https://doi.org/10.33003/fjs-2025-0910-3957

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