GENETIC RELATEDNESS OF ESBL AND NON-ESBL SALMONELLA TYPHIMURUM ISOLATED FROM POULTRY BIRDS AND POULTRY HANDLERS IN NASARAWA STATE, NIGERIA

Authors

Keywords:

Genetic relatedness, PCR-RFLP, 16S rRNA gene, ESBL, salmonella typhimurium, Nasarawa, poultry

Abstract

Multidrug resistant salmonellosis infection is an important global public health problem particularly in sub-Saharan Africa, where it commonly manifests as gastroenteritis and/or bloodstream infections in both children and adults. Salmonella Typhimurium has a broad-host-range and poultry birds are an important reservoir. The unrestricted use of Beta-lactams has generated resistance through a gene encoded Extended Spectrum Beta-lactamase (ESBL) enzyme production, which impedes the successful therapy of infections. This study aims at investigating the genetic relatedness of ESBL and non-ESBL S. Typhimurium strains isolated from humans and Poultry birds. A total of 19 S. Typhimurium isolated from Poultry (droppings, flesh, feeds) and handlers (feces, hand swabs) in a previous study were used. These had earlier been identified using pre-enrichment and selective enrichment culture media. Further identification was carried out using conventional biochemical screening tests and slide serology tests using polyvalent antisera. Antibiotic susceptibility testing including the Double Disk Synergy test (DDST) for screening of ESBL production was carried out as described by the Clinical and Laboratory Standards Institute (CLSI) guidelines. This study also employed the PCR-RFLP method which involves the simple restriction digestion of purified 16S rRNA of Salmonella and variation in the banding patterns revealed their genetic relatedness. All the strains were found to share identical molecular base profiles hence are genetically related variants. The selective pressure due to antibiotic mismanagement in Poultry farming is the likely trigger of ESBL production observed to occur only among the S. Typhimurium strains from poultry origin. This calls for a holistic approach in

Dimensions

Akbarmehr, J., Salehi, T.Z., & Nikbakht Brujeni, G.N. (2010). Identification of Salmonella isolated from poultry by MPCR technique and evaluation of their hsp groEL gene diversity based on the PCR-RFLP analysis. African J Microbiol Res.4, 1594-98

Card, R., Vaughan K., Bagnall, M., Spiropoulos, J., Cooley, W., Strickland, T., Davies, R., & Anjum, M.F. (2016). Virulence, Characterization of Salmonella enterica isolates of differing antimicrobial resistance recovered from UK Livestock and imported meat samples. Front Microbiol. (7), 640.doi:10.3389/fmicb.2016.00640

Chatterjee, S. & Raval, I.H. (2019). Pathogenic Microbial Genetic Diversity with Reference to Health, in Microbial Diversity in the Genomic Era. Section I. In Surajit Das & Hirak Dash (Eds.) Microbial Diversity in the Genomic Era. (pp 3-14). [e-book].Retrieved from https://www.sciencedirect.com/topics/immunology-and-microbiology/restriction-fragment-length-polymorphism.

Chenggang, X., Xingxing, R., Zhou F., Ying, F., Yanfen H., Zujie, S.,… & Jianmin, Z. (2017). Phenotypic characteristics and Genetic diversity of Salmonella enterica serotype Derby isolated from human patients and foods of animal origin. Food borne Pathogens and Disease, 14, No. 10. doi.org/10.1089/fpd.2017.2278

Chowdhury, N., Asakura, M., Neogi, S.B., Hinenoya, A., Haldar, S., Ramamurthy, T., Sarkar, B.L., Faruque, S.M., & Yamasak, S. (2010). Development of simple and rapid PCR-fingerprinting methods for Vibrio cholera on the basis of genetic diversity of the super integron (2010). Journal of Applied Microbiology 109 (2010) 304–312

Cosby, D.E., Cox, N.A., Harrison, M.A., Wilson, J.L., Buhr, R.J., & Fedorka-Cray, P.J. (2015). Salmonella and antimicrobial resistance in broilers: A review. J Appl Poult Res. 2015; 24: 408-426.

Dilmaghani, M., Ahmadi, M., Zahraei S.T., Talebi, A., & Darvish Z.R. (2010). PCR-Restriction fragment length polymorphism analysis of fljB gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avian. Iranian J Microbiol. 2,178-184.

Feasey, N.A, Dougon, G., Kingsley, R.A., Heyderman, R.S., Gordon, M.A. (2012). Invasive non-typhoidal Salmonella disease: an emerging and neglected tropical disease in Africa. Lancet 379: 2489-2499.doi.10.1016/S0140-6736(11) 61752-2

Guibourdenche, M., Roggentin, P., Mikoleit, M., Fields, P.I., Bockemuh, l J., Grimont, P.A., & Weill, F.X. (2010). Supplement 2003–2007 (No. 47) to the White- Kauffmann-Le Minor scheme. Research in Microbiology. 2010;161:26–29.doi: 10.1016/j.resmic.2009.10.002

Ibrahim, T., Ngwai, Y.B., Pennap, G.R.I., Ishaleku, D., Tsaku, P.A., Abimiku, R.H., Nkene, I.H., & Bassey, E.B. (2019). Antimicrobial Resistance Profile of Salmonella Typhimurium Isolated from Commercial Poultry and Poultry farm handlers in Nasarawa State, Nigeria. Microbiology Research Journal International 28(4):1-12

Issenhuth-Jeanjean, S., Roggentin, P., Mikoleit, M., Guibourdenche, M., Pinna, E, Nair, S., Fields, P.I. & Weill, F.X. (2014). Supplement 2008-2010 (no. 48) to the White-Kauffmann-Le Minor scheme. Res. Microbiol. 165: 526-530.

Jiang, H., Dong H., Zhang G., Yu B., Chapman L. R., & Fields M. W. (2006). Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Appl. Environ. Microbiol. 72, 3832–3845

Khaki, P., Moradi, S.B., & Elham, E. (2013). PCR-RFLP of isolated Salmonella from poultry with Sau3AI and Hhal restriction endonucleases in Arak. Int J Mol and Clin Microbio 1(2013)255-260

Landers, T.F., Cohen, B., Wittum, T.E., & Larson, E.L. (2012). A review of Antibiotic Use in Food Animals: Perspective, Policy and Potential. Public Health Rep. 127(1):4-22

Lu, Y., Zhao, H., Sun, J., Liu, Y., Zhou, X. & Beier, R. C. (2014). Characterization of multidrug-resistant Salmonella enterica serovars Indiana and Enteritidis from chickens in Eastern China. PLoS ONE 9:e96050.

Matsui, T., Matsuda, M., Murayama, O., Miller, B.B., & Moore, J.E., (2001). RecA genotyping of Salmonella enteriditis phage type4 isolates by restriction fragment length polymorphism analysis. Lett Appl Microbiol. 32, 424-427.

Moradi, S., Cheraghchi, N., Khaki, P, & Sabokbar, A. (2014). Identification of isolated Salmonella enterica serotype gallinarum biotype pullorum and gallinarum by PCR-RFLP. Jundishapur journal of microbiology, 7(9):e19135

Mukherjee, S., Anderson, C.M., Mosci, R.E., Newton, D.W., Lephart, P., Salimnia, H., Khalife, W., Rudrik, J.T., & Manning, S.D. (2019). Increasing frequencies of antibiotic resistant non-typhoidal Salmonella infections in Michigan and risk factors for disease Front. Med. Retrieved from https://doi.org/10.3389/fmed.2019.00250

Neslihan, T. K., Fatima, N.Y., Nefise A., & Mustafa, A. (2018).Genetic diversity of food originated Salmonella isolates. Biotechnology & Biotechnologival equipment. 32(3), 638-645.doi:10.1080/13102818.2018.1451779.

Oyinloye, J.M. (2011). Extended spectrum beta-lactamase (ESBL) - producing Multidrug resistant Enterobacteriaceaea from commercial Poultry feeds in Nigeria. Annals of Biological Research, 2:250-254

Rabsch, W., Andrews, H.L., Kingsley, R.A., Prager, R., Tschape, H., Adams, G.L., & Baurmier, A. J. (2002). Salmonella enterica serotype Typhimurium and its hosted-adapted variants. Infection and immunity, 70 (5) 2249-2255.

Shaikh, S., Fatima, J., Shakil, S., Mohd, .S., Rizvi, D., & Kamal, A.J. (2015). Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi Journal of Biological Sciences (22), 90–101

Scallan, E., Hoekstra, R.M., Angulo, F.J., Tauxe, R.V., Widdowson, M.A. & Roy, S.L. (2011). Foodborne illness acquired in the United States-major pathogens. Emerg. Infect. Dis. 17 (1): 7-15.

Soler-García, A. A., De Jesús, A. J., Taylor, K., & Brown, E. W. (2014). Differentiation of Salmonella strains from the SARA, SARB and SARC reference collections by using three genes PCR-RFLP and the 2100 Agilent Bioanalyzer. Frontiers in microbiology, 5, 417. https://doi.org/10.3389/fmicb.2014.00417

Sumithra, T.G., Chaturvedi, V.K., & Gupta, P.K (2014). PCR-RFLP analysis of fliC, fimH Band 16s r RNA genes in Salmonella Typhimurium isolates of varied origin. Ann Microbiol. 2014; 64:177-183.

Turki Y., Mehri I., & Fhoula I. (2014). Comparison of five molecular subtyping methods for differentiation of Salmonella Kentucky isolates in Tunisia. World J Microbiol Biotechnol. 2014;30:87–98

Zaki, S., Abd-El-Haleem, D., & El-Helow, E (2009). Molecular and Biochemical diagnosis of Salmonella in waste water. J Appl Sci Environ Manage.2009;13:83-92

World health organization. (WHO). (2018). Non-typhoidal Salmonella. Retrieved from: https://www.who.int/news-room/fact-sheets/detail/Salmonella- (non-typhoidal).

Published

23-09-2020

How to Cite

GENETIC RELATEDNESS OF ESBL AND NON-ESBL SALMONELLA TYPHIMURUM ISOLATED FROM POULTRY BIRDS AND POULTRY HANDLERS IN NASARAWA STATE, NIGERIA. (2020). FUDMA JOURNAL OF SCIENCES, 4(3), 201-206. https://doi.org/10.33003/fjs-2020-0403-386

Issue

Vol. 4 No. 3 (2020): FUDMA Journal of Sciences - Vol. 4 No. 3

Section

Research Articles
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FUDMA Journal of Sciences

How to Cite

GENETIC RELATEDNESS OF ESBL AND NON-ESBL SALMONELLA TYPHIMURUM ISOLATED FROM POULTRY BIRDS AND POULTRY HANDLERS IN NASARAWA STATE, NIGERIA. (2020). FUDMA JOURNAL OF SCIENCES, 4(3), 201-206. https://doi.org/10.33003/fjs-2020-0403-386

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