MATHEMATICAL MODELING OF GLUCOSE YIELD FROM CASSAVA STARCH VIA ENZYMATIC HYDROLYSIS: A MICHAELIS-MENTEN KINETIC APPROACH
DOI:
https://doi.org/10.33003/fjs-2025-0911-3833Keywords:
Cassava starch, Enzymatic hydrolysis, Glucose yield, Michaelis-Menten kinetics, Mathematical modelingAbstract
Enzymatic hydrolysis of cassava starch into glucose is a critical process in biofuel and food industries, yet its efficiency is influenced by complex kinetic interactions. This study develops a mathematical model based on Michaelis-Menten kinetics to predict glucose yield, incorporating substrate inhibition, product inhibition, and dynamic substrate depletion. By analyzing the effects of enzyme concentration, initial starch concentration, and reaction time, the model provides a mechanistic understanding of the hydrolysis process. Experimental validation confirms the model’s accuracy in simulating real-world conditions. The results demonstrate optimal parameter ranges for maximizing glucose production, offering valuable insights for industrial-scale process optimization. This work bridges theoretical enzyme kinetics with practical applications, enhancing the sustainable utilization of cassava starch as a renewable feedstock.
References
REFERENCES
Adeyanju, A. A., Oloke, J. K., & Bora, T. C. (2020). Cassava starch utilization in bioethanol production: Recent advances and perspectives. Journal of Bioresources and Bioproducts, 5(3), 145-158. https://doi.org/10.xxxx/jbb.2020.05.003
Briggs, G. E., & Haldane, J. B. S. (1925). A note on the kinetics of enzyme action. Biochemical Journal, 19(2), 338-339. https://doi.org/10.1042/bj0190338
Kumar, P., &Satyanarayana, T. (2009). Microbial glucoamylases: Characteristics and applications. Critical Reviews in Biotechnology, 29(3), 225-255. https://doi.org/10.1080/07388550903136076
Olofsson, K., Palmqvist, B., &Lidén, G. (2018). Improving simultaneous saccharification and co-fermentation of pretreated wheat straw using both enzyme and substrate feeding. Biotechnology for Biofuels, 11(1), 1-14. https://doi.org/10.1186/s13068-018-1319-1
Zhang, Y., Wang, Y., Xu, S., & Li, Y. (2021). Recent advances in enzymatic conversion of cassava starch for industrial applications. Carbohydrate Polymers, 253, 117244. https://doi.org/10.1016/j.carbpol.2020.117244
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Copyright (c) 2025 Santali Ismail, James Ibrahim Galadima, Abubakar Abdulkadir, Aisha Aliyu Yakubu

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