IN VITRO AMELIORATIVE POTENTIAL OF SOME ORGANIC ACID IDENTIFIED FROM BOSWELLIA DALZIELII STEM BARK EXTRACT AGAINST FREE RADICALS
Abstract
Due to their safety and availability, medicinal plants are natural alternatives to orthodox medicines in health management, particularly in developing countries. One such plant is Boswellia dalzielii, a renowned tree in northern Nigeria that is extensively used due to its ethnomedicinal history. This study aimed to evaluate the antioxidant of some organic acids identified in the stem bark extract of B. dalzielii by Liquid Chromatography Mass Spectrometer (LCMS). The stem bark was extracted with ethanol using the cold maceration method. Antioxidant assay using 2,2-Diphenyl 1-Picryl-Hydrazyl-hydrate (DPPH), Ferric Reducing Antioxidant Property (FRAP), and Hydrogen Peroxide (H2O2) methods were used, and the results showed the highest antioxidant activity with higher concentrations of the extract; 49.580% ± 0.214, 3.540 ± 0.022 and 45.145% ± 0.234 for DPPH, FRAP and H2O2 respectively. And significantly lower than the standard (Ascorbic acid) used, 95.01 ± 0.001, 3.540 ± 0.120, and 91.390%± 0.022 respectively. the LCMS profile revealed the presence of many organic acids and other metabolites; Malonamic acid, D-pyroglutamic acid, Maleamic acid, Benzohydroxamic acid, 2-bromo-1,10-phenanthroline, p-tropoquinone (Quinone derivatives) among others. Therefore, the stem bark of B. dalzielii extract possesses antioxidant potential and could be due to its organic acid presence, it can be concluded that the stem bark of this plant may perhaps be useful in health management.
References
Abubakar, H., Tahir, A. and Umar, A. K. (2024). Boswellia dalzielii Methanol Stem Bark Extract Demonstrated Significant Analgesic Activity in Swiss Albino Mice. Sciences of Phytochemistry 3(1): 38-43. DOI: https://doi.org/10.58920/sciphy0301225
Chen, L., et al. (2018). Antioxidant activity of 2-bromo-1,10-phenanthroline and its metal complexes. Journal of Chemical and Pharmaceutical Research, 10(1), 219-228.
Desta G.T, Ferede Y.A, Zewdu W.S, Adugna B.Y, Arega T, Alemu M.A. (2022). Validation of antidiabetic and antihyperlipidemic effects of 80% methanolic extract of the lonchocarpus laxiflorus leaves in streptozotocin-induced diabetic Swiss albino mice. Evid base Compl Alternative Med 2022:19. DOI: https://doi.org/10.1155/2022/8411851
Dogara A.M, Lema A.A., Hama H.A., Hamad S, Mohammad N.H.M., and Khandaker, M. M. (2022). Ethnopharmacology, Biological evaluation and Chemical composition of Boswellia dalzielii Hutch: A Review. Indonesian J Pharm. 20;51539. DOI: https://doi.org/10.22146/ijp.4279
Fatima N, Anwar F, Saleem U, Khan A, Ahmad B, Shahzadi I, et al. (2022). Antidiabetic effects of Brugmansia aurea leaf extract by modulating the glucose levels, insulin resistance, and oxidative stress mechanism. Front Nutr 9:1005341 DOI: https://doi.org/10.3389/fnut.2022.1005341
Garba, Y., Bello, N.U., Bashir, M. and Mohammed, S. U. (2023). In vitro and In vivo analysis of the antioxidant potentials of methanolic extract of Tamarindus indica leaves. Nigerian Journal Of Biochemistry and Molecular Biochemistry 38(3), 104-111 DOI: https://doi.org/10.4314/njbmb.v38i3.1
Hamadjida, A., Ayissi Mbomo, R. E., Minko, S. E., Ntchapda, F.. Kilekoung Mingoas, J. P. and Nnanga, N. (2024). Metabolism Open 21 100278 DOI: https://doi.org/10.1016/j.metop.2024.100278
Huang, W., et al. (2018). Antioxidant activity of D-pyroglutamic acid and its derivatives. Journal of Food Science, 83(5), S1448-S1456.
Karl Egil Malterud (2017). Ethnopharmacology, Chemistry and Biological Properties of Four Malian Medicinal Plants Plants, 6, 11; https://doi.org/10.3390/plants6010011 DOI: https://doi.org/10.3390/plants6010011
Kim, J., et al. (2019). Antioxidant and anti-inflammatory activities of D-pyroglutamic acid. Journal of Medicinal Food, 22(10), 1039-1046.
Kumar, P., et al. (2018). Antioxidant activity of malonic acid and its derivatives. Journal of Agricultural and Food Chemistry, 66(2), 533-541.
Kumar, P., et al. (2019). Antioxidant activity of maleamic acid and its metal complexes. Journal of Chemical and Pharmaceutical Research, 11(1), 209-218.
Lee, S., et al. (2020). Antioxidant activity of D-pyroglutamic acid and its metal complexes. Journal of Chemical and Pharmaceutical Research, 12(2), 249-258.
Liu, X., et al. (2020). Synthesis, characterization, and biological evaluation of 2-bromo-1,10-phenanthroline derivatives as antioxidants. European Journal of Medicinal Chemistry, 187, 112046.
Manoharan Karuppiah Pilla, and Khombisile H Simelane (2024). Phytochemical Analysis and Antioxidant Activity of Extracts from Berchemia zeyheri A Swazi Medicinal Plant Qeios, CC-BY 4.0 1-11. DOI: https://doi.org/10.32388/PEXTMK
Manye, S. J., Sal, J. H., Ishaya, H. B., Chiroma, S. M.,Attah, M. O.C. and Dibal, N. I., (2023). Pharmacological Research - Modern Chinese Medicine 8 : 100291 DOI: https://doi.org/10.1016/j.prmcm.2023.100291
Muhammad, I.; Yang, L.; Ahmad, S.; Mosaad, I.S.M.; Al-Ghamdi, A.A.; Abbasi, A.M.; Zhou, X.-B. (2022). Melatonin Application Alleviates Stress-Induced Photosynthetic Inhibition and Oxidative Damage by Regulating Antioxidant Defense System of Maize: A Meta-Analysis. Antioxidants, 11, 512. https://doi.org/10.3390/antiox11030512 DOI: https://doi.org/10.3390/antiox11030512
Ojo OA, Oni AI, Grant S, Amanze J, Ojo AB, Taiwo OA, et al. (2022). Antidiabetic activity of elephant grass (Cenchrus purpureus (Schumach.) Morrone) via activation of PI3K/AkT signaling pathway, oxidative stress inhibition, and apoptosis in Wistar rats. Front Pharmacol 13:845196. DOI: https://doi.org/10.3389/fphar.2022.845196
Owolabi M.S., Ogundajo, A., Solomon B.O., Olatunde L, Dosoky N.S. and Setzer W.N. (2020). Essential Oil Compositions, Antibacterial and Antifungal Activities of Nigerian Members of the Burseraceae: Boswellia dalzielii and Canarium schweinfurthii. Natural Product Communications. 2020 Aug 1;15(8):1934578X20946940. DOI: https://doi.org/10.1177/1934578X20946940
Patel, M., et al. (2019). Antioxidant activity of malonic acid and its metal complexes. Journal of Chemical and Pharmaceutical Research, 11(1), 200-208.
Rajendran, R., et al. (2018). Antioxidant activity of maleamic acid and its derivatives. Journal of Agricultural and Food Chemistry, 66(2), 542-549.
Sharma, P., et al. (2020). Antioxidant activity of benzohydroxamic acid and its metal complexes. Journal of Chemical and Pharmaceutical Research, 12(1), 229-238.
Singh, J., et al. (2020). Synthesis, characterization, and biological evaluation of malonic acid derivatives as antioxidants. European Journal of Medicinal Chemistry, 187, 112044.
Singh, R., et al. (2017). Antioxidant activity of benzohydroxamic acid and its derivatives. Journal of Agricultural and Food Chemistry, 65(2), 533-541.
Sofowora A. (2008). Medicinal Plants and Traditional Medicine in Africa. 3rd edn. Spectrum Books, Ibadan.
Srivastava, P., et al. (2020). Synthesis, characterization, and biological evaluation of maleamic acid derivatives as antioxidants. European Journal of Medicinal Chemistry, 187, 112045.
Taylor, J.L.S., Rabe, T., McGaw, L.J., Jger, A.K., Van, S.J. (2001). Towards the scientific validation of traditional medicinal plants. Plant Growth Regulation 2337.
Wachtel-Galor S, Benzie IFF. (2011). Herbal Medicine: An Introduction to Its History, Usage, Regulation, Current Trends, and Research Needs. In: Benzie IFF, WachtelGalor S, editors. Herbal Medicine: Biomolecular and Clinical Aspects [Internet]. 2nd ed. Boca Raton (FL): CRC Press/Taylor & Francis; [cited 2024 Feb 21]. Available from: http://www.ncbi.nlm.nih.gov/books/NBK92773/
Zhang, Y., et al. (2019). Antioxidant activity of 2-bromo-1,10-phenanthroline and its derivatives. Journal of Agricultural and Food Chemistry, 67(2), 533-541.
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