ANTIBACTERIAL STUDIES OF ESSENTIAL OIL FROM THE FRESH LEAF OF LEMON GRASS CYMBOPOGON CITRATUS
Abstract
Cymbopogon citratus, belongs to Gramineae family. This study used a microwave-assisted hydro-distillation process to extract lemongrass essential oil and examined its antimicrobial qualities. Physical characteristics of the essential oils included a yellow color, a yield percentage of 4.67%, solubility in trichloromethane, and a lemony aroma. The disc diffusion method was used to assess the oil's effectiveness. Pseudomonas aeruginosa, Klebsiella oxytoca, Staphylococcus aureus, and Escherichia coli were all susceptible to the oil's concentration-dependent antibacterial qualities. 5.72 mmL/disc was the oil's most effective concentration against E. coli, while 1.43 mmL/disc was its least effective. The zone of inhibition shrank as the concentration of oil per disc dropped, indicating that the oil's activity against all species was concentration-dependent. At concentrations of 5.72 mmL/disc, 2.86 mmL/disc, and 1.43 mmL/disc, respectively, the zone of inhibition for E. coli was 24, 11.3, and 7.7 mm. For other creatures, the pattern is the same. Pseudomonas aeruginosa had the smallest zone of inhibition, measuring 7.0, 7.0, and 5.7 mm at concentrations of 5.72 mmL/disc, 2.86 mmL/disc, and 1.43 mmL/disc, respectively. Staphylococcus aureus was 13.3, 10.3, and 9.0 mm at concentrations of 5.72 mmL/disc, 2.86 mmL/disc, and 1.43 mmL/disc, while Klebsiella oxytoca was 11.3, 9.7, and 9.0 mm at concentrations of 5.72 mmL/disc, 2.86 mmL/disc, and 1.43 mmL/disc, respectively. These results imply that lemongrass essential oil may be a viable natural substitute for synthetic antibiotics, with potential uses in medical and food preservation. It is advised that additional bioassays be conducted and contrasted with the results obtained from alternative...
References
Abdullahi, M. A., Sani, M. I., and Ahmad, N. S., (2024). Biological investigation and chemical constituents of Croton nigritanus scott Elliot. Journal of Pharmacognosy and Phytochemistry 13(1):32-37. https://doi.org/10.22271/phyto.2024.v13.i1a.14810 DOI: https://doi.org/10.22271/phyto.2024.v13.i1a.14810
Abdullahi, M. A., Olime M. N., Usman G., Muhammad A. M., Muhammad I. N., Muhammad B. U., and Umar M. I., (2024). Isolation of Squalene from Dichloromethane Extract of Croton Nigritanus Leaf. FUDMA Journal of Sciences (FJS). 8(2): 212-216 DOI: https://doi.org/10.33003/fjs-2024-0802-2049
https://doi.org/10.33003/fjs-2024-0802-
Abers, M., Schroeder, S., Goelz, L., Sulser, A., St. Rose, T., Puchalski, K., & Langland, J. (2021). Antimicrobial activity of the volatile substances from essential oils. BMC Complementary Medicine and Therapies, 21:124. https://doi.org/10.1186/s12906-021-03285-3 DOI: https://doi.org/10.1186/s12906-021-03285-3
Ali, M. M., Yusuf, M. A. & Abdalaziz. M. N. (2017). GC-MS Analysis and Antimicrobial Screening of Essential Oil from Lemongrass (Cymbopogon citratus). International Journal of Pharmacy and Chemistry. 3(6). 72-76. https://doi.org/10.11648/j.ijpc.20170306.11 DOI: https://doi.org/10.11648/j.ijpc.20170306.11
Andrews J. M. (2001). Determination of minimum inhibitory concentrations. The Journal of Antimicrobial Chemotherapy, 48 (1), 5 16. https://doi.org/10.1093/jac/48.suppl_1.5 DOI: https://doi.org/10.1093/jac/48.suppl_1.5
Atiko, R., Kwaji, A. Yoriyo, K. P. &. A. (2016). Chemical Composition and Larvicidal Activity of Hyptis spicigera Volatile oils against Mosquito Larvae: Anopheles gambiae and Culex quinquefasciatus Say International Journal of Scientific and Engineering Research 7(7):888-891
Cardoso-Ugarte, G. A., Jurez-Becerra, G. P. Sosa-Morales, M. E. & Lpez-Malo, A. (2013). Microwave-assisted Extraction of Essential Oils from Herbs. Journal of Microwave Power and Electromagnetic Energy, 47(1): 63-72 DOI: https://doi.org/10.1080/08327823.2013.11689846
Chavez-Gonzalez, M. L., Rodriguez-Herrera, R., Aguilar, C. N., (2016). Essential Oils: A natural alternative to combat antibiotics resistance. Mechanism and New Antimicrobial Approaches. 227-237. DOI: https://doi.org/10.1016/B978-0-12-803642-6.00011-3
Dibala, C. I., Konate, K., Diao, M., Maurice, O. M. and Dicko. (2014). Phytoconstituents Analysis, Antioxidant capacity and Antimicrobial properties of extracts from Chrozophora senegalensis. Journal of Pharmacy and Pharmaceutical Sciences 6(7):172-178.
Doughari, J. H., Human, I. S., Bennade, S., & Ndakidemi, P. A. (2009). Phytochemicals as chemotherapeutic agents and antioxidants: Possible solution to the control of antibiotic resistant verocytotoxin producing bacteria. Journal of Medicinal Plants Research. 3(11):839848. http://www.academicjournals.org/jmpr
Dutta K. N., Chetia P., Lahkar, S. and Das, S. (2013). Herbal plants used as diuretics: A comprehensive review. Journal of Pharmacological, Chemical and Biological Sciences, 2(1):27-32.
Grant, A., & Mierzejewski, K. (2023, May 4). Lemongrass Herbs: Learn About Growing A Lemongrass Plant. Gardening know how. https://www.gardeningknowhow.com/edible/herbs/lemongrass/learn-about-growing-lemon-grass-plant.htm
Licien, B. V., Douglas, M. H., Scott, P. M., John, K. H., and Michael, J. M., (2023). Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics. Star Protocols. 4(3): 102512. DOI: https://doi.org/10.1016/j.xpro.2023.102512
Man, A., Santacroce, L., Jacob, R., Mare, A., & Man, L. (2019). Antimicrobial Activity of Six Essential Oils against a Group of Human Pathogens: A Comparative Study. Pathogens (Basel, Switzerland), 8(1): 9-15. https://doi.org/10.3390/pathogens8010015 DOI: https://doi.org/10.3390/pathogens8010015
Masyita, A., Mustika Sari, R., Dwi Astuti, A., Yasir, B., Rahma Rumata, N., Emran, T. B., Nainu, F., & Simal-Gandara, J. (2022). Terpenes and Terpenoids as Main Bioactive Compounds of Essential Oils, their Roles in Human Health and Potential Application as Natural Food Preservatives. Food chemistry: 13,100217. DOI: https://doi.org/10.1016/j.fochx.2022.100217
Morais, D., S. M., Facundo, V. A., Bertini, L. M., Cavalcanti, E. S. B., Anjos Jnior, J. F. D., Ferreira, S. A., de Brito, E. S., & de Souza Neto, M. A. (2007). Chemical Composition and Larvicidal Activity of Essential Oils from Piper Species. Biochemical Systematics and Ecology, 35(10), 670675. https://doi.org/10.1016/j.bse.2007.05.002 DOI: https://doi.org/10.1016/j.bse.2007.05.002
Mukherjee, S., Gurjar, E. S., Gowda, K. V., Navyashree, G. S., Shet, S., Mishra, S. & Kumar, S. (2024) Lemongrass: A Traditional Ethno-Medicinal Plant of India. Medicinal Poaceae of India, 1; 10-19
Okpo, S. O., Edeh, F., (2023). A comprehensive review on Lemon grass (Cymbopogon citratus) Oil Extraction and its Applications. EPRA International Journal of Research and Development(IJRD). 8(4): 258-273. DOI: https://doi.org/10.36713/epra12959
Oladeji, O. S., Adelowo, F. E., Ayodele, D. T., & Odelade, K. A. (2019). Phytochemistry and pharmacological activities of Cymbopogon citratus: A review. Scientific African, 6, e00137. https://doi.org/10.1016/j.sciaf.2019.e00137 DOI: https://doi.org/10.1016/j.sciaf.2019.e00137
Rios, J. L., (2016). Essential Oils. Essential Oils in Food Preservation, Flavor and Safety, 310. https://doi.org/10.1016/b978-0-12-416641-7.00001-8 DOI: https://doi.org/10.1016/B978-0-12-416641-7.00001-8
Robenson, M. M. and Zhang, X. (2011). The world medicine situation, traditional medicine: global situation, issues and challenges. Available from http://www.oalib.com/references/8513138
Scott, R. P. W. (2005). Essential Oils. Encyclopedia of Analytical Science, 554561. https://doi.org/10.1016/B0-12-369397-7/00147-3 DOI: https://doi.org/10.1016/B0-12-369397-7/00147-3
Shah, G., Shri, R., Panchal, V., Sharma, N., Singh, B. & Mann, A. S. (2011). Scientific Basis for the Therapeutic use of Cymbopogon citratus, Stapf (Lemongrass). Journal of Advanced Pharmaceutical Technology & Research 2(1):3-8 https://doi.org/10.4103/2231-4040.79796 DOI: https://doi.org/10.4103/2231-4040.79796
Shukr, M. H & Metwally, G. F. (2013). Evaluation of Topical Gel Bases Formulated with Various Essential Oils for Antibacterial Activity against Methicillin Resistant Staphylococcus Aureus. Tropical Journal of Pharmaceutical Research. 12(6):877-884. http://dx.doi.org/10.4314/tjpr.v12i6.3 DOI: https://doi.org/10.4314/tjpr.v12i6.3
Sofowora, A., Ogunbodede, E., & Onayade, A. (2013). The role and place of medicinal plants in the strategies for disease prevention. African Journal of Traditional Complementary and Alternative Medicines, 10(5). https://doi.org/10.4314/ajtcam.v10i5.2 DOI: https://doi.org/10.4314/ajtcam.v10i5.2
Uraku, A. J., (2015). Determination of chemical compositions of Cymnopogon citratus Leaves by Gas Chromatography-Mass Spectrometry (GC-MS) method. Research Journal of Phytochemistry. 9(4):175-187. DOI: https://doi.org/10.3923/rjphyto.2015.175.187
Webber, D. M., Wallace, M. A., Burnham, C. D., (2022). Stop waiting for tomorrow: Disk diffusion performed on early growth is an accurate method for antimicrobial susceptibility testing with reduced turnaround time. Journal of Clinical Microbiology. 60(5): 115-120. DOI: https://doi.org/10.1128/jcm.03007-20
Yohanna, C., Kwaji, A., & Atiko, R. (2021). Antibacterial Activity, Antioxidant Potential and Stigmasterol Isolation from Laggera aurita Linn (Asteraceae). International Journal of Biochemistry Research and Review, 30(9):2439. https://doi.org/10.9734/ijbcrr/2021/v30i930288 DOI: https://doi.org/10.9734/ijbcrr/2021/v30i930288
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