• Abdullahi Muhammad Abdullahi Federal University of health sciences Azare Bauchi Nigeria
  • Olime M. Nkemakonam Department of Chemical Sciences Pen Resource University Gombe
  • Usman Garba Department of Chemistry Federal University of Agriculture Makurdi Benue state
  • Muhammad A. Mustapha
  • Muhammad I. Nazifi
  • Muhammad B. Usman
  • Umar M. Ibrahim
Keywords: Isolation, Terpenes, Croton, Extraction, Spectroscopy


A precursor of steroids with a variety of biological activities is squalene. The purpose of the study was to identify and describe the industrial/medical chemical found in Croton nigritanus. Using the solvents such as; dichloromethane, ethyl acetate, and n-hexane, the procedure entails a liquid-liquid extraction stage using the partition method and a solid-liquid extraction step using the maceration method. For separation and purification, gravity column chromatography (GCC) and thin-layer chromatography were employed. The isolated chemical's identity was determined using FTIR, 1H NMR and GC-MS spectroscopy. The inquiry successfully extracted and analyzed the major chemical compound which was identified as Squalene.

Author Biography

Abdullahi Muhammad Abdullahi, Federal University of health sciences Azare Bauchi Nigeria

Department of Chemistry faculty of Basic Sciences Federal University of health sciences Azare Bauchi State Nigeria. Graduate Assistant 


Abeje A, Fikre M and Belayhun K., (2016): Isolation and characterization of terpene from leaves of Croton macrostachyus (Bissana). Journal of Medicinal Plants Research. 10(19): 256-260.

Abdulrahman H, Amina E, Ibrahim A, and Mahmoud E (2022): A Review Article of Phytochemical Constitutions of Croton Genus. European Journal of Pharmaceutical and Medical Research. ,9(3), 64-67.

Abeye A., Fikre M., and Belayhun K. (2016). Isolation and characterization of terpene from leaves of Croton macrostachyus (Bissana). Journal of Medicinal Plants Research. 10(19): 256-269.

Akpotu M, O, Eze PM, Abba C, C, Umeokoli B, O, Nwachukwu C, U, Okoye F, B, Esimone C, O. (2017): Antimicrobial activities of secondary metabolites of endophytic fungi isolated from Catharanthus roseus. J Health Sci. 7(1):15-22. DOI:

Alfred Maroyi (2019): Croton species in Madagascar: their ethnomedicinal uses, phytochemistry and biological activities. Asian J Agric & Biology. 7(2):279-288.

Andi R, R, Anis A A, Rozida K, Rizafizah O, Azwan M, Iqbal M. C, Yana M S, Jalifah L, Ikram M. S, Muntaz A B (2019): Isolation of New Rotundone from the Roots of Croton Hirtus (euphorbiaceae). Malaysian journal of analytical sciences, 23(4): 677 - 681 doi:

Ardhyni. D. H., Aparamarta H. W., Widjaja. A., Ibrahim R., and Gunawan S. (2022): The extraction and purification of squalene from Nyamplung (Calophyllum Inophyllum L) Leaves. IOP Conference Series: Earth and Environmental Science, Volume 963, International Bioprocessing Association subject Conference (IBASC 2021) 4th-5th August 2021, Yogyakarta Indonesia. Doi: 10.1088/1755-1315/963/1/012042. DOI:

Dorine N. M, Joseph M. N., Fredrick M. M, Isaac M., Hashim M. A., Vincent M. O. (2021): Botanical description, ethnomedicinal uses, phytochemistry and pharmacological effects of Croton dichogamus Pax (Euphorbiaceae). The Journal of Phytopharmacology 10(1): 42-47. DOI:

Emmanuel M., N, Jacobus N. E, and Lyndy J. M. (2018): Croton gratissimus leaf extracts inhibit cancer cell growth by inducing caspase 3/7 activation with additional anti-inflammatory and antioxidant activities. BMC complementary and alternative medicine.18:305. DOI:

Ermias M. T, Faith A. O, Solomon D. , Moses K. L, Eduard M., Kamal A. Q, Mariusz J., and Joseph M (2022): Anti-HIV Ermiasolides from Croton megalocarpus 27, 7040. DOI:

Fatemeh H., Nafiseh F., Mohammad K., Mahmoud E. (2018): A novel method for squalene extraction from pumpkin seed oil using magnetic nanoparticles and exploring the inhibition effect of extracted squalene on angiogenesis property. Journal of the Taiwan Institute of Chemical Engineers. 91(5): 1-9. DOI:

Ferdinand, C., Isyaka, M. S, Tiwa A. A, Ibekwe, N. N., John I., and Abdullahi M. A. (2023): Isolation of Sitosterol and Stigmasterol from The Roots of Croton Penduliflorus. FUDMA Journal of Sciences (FJS). 7(6): 52 – 56 DOI:

Josephat T. (2021): Invitro antifungal susceptibility of yeasts and Molds isolated from sputum of tuberculosis relapse and retreatment patients. Pan African Medical Journal. 38:227. DOI:

Meresa A, Rekik A, Worku G, Firehiwot T, Hirut B, Abiy A, Eyob D, Samuel W and Ashenif T (2019): Ethno medicinal uses, phytochemistry and anti-malarial effect of Croton macrostachyus (Bisana): A review. Journal of Medicinal Plants Studies 7(2): 79-88.

Nnamdi M. A, Ifeanyi P. O, Chika C. A, Nkeoma N. O , Peter M. E, Blessing O. U, OgechI O. A and Festus C. O (2022): Antimicrobial Activities of the Endophytic Fungus, Fusarium Equiseti, Isolated from The leaves of Ocimum gratissimum. Journal of Advances in Medical and Pharmaceutical Science. 24(7): 11-23.

Sonia M, E, Adolfo A. C, Daniel G, R and Roberto A, E(2022): Phytochemical Screening and Isolation of New Ent-Clerodane Diterpenoids from Croton guatemalensis Lotsy. Plants 11:3159. DOI:

Wen, H, X, Wei, Y. L and Qian L. T (2018): Chemical Constituents from Croton Species and Their Biological Activities. International journal of Brazilian chemical society. 5(3): 53-60.

Yue. Y. S., Jing. X., kai. R. W., Ze. Y. L., Kai. L., Jian. L. L., Jian. H. Y., Yue. R. L., Xin. Q. Z. (2022): Extraction of Squalene From Tea Leaves (Camellia sinensis) and its variations with leaf Maturity and Tea Cultivar. Frontiers. Nutr, 9: 755514. Doi: 10.3389/fnut.2022.755514. DOI:

How to Cite
AbdullahiA. M., Nkemakonam O. M., GarbaU., MustaphaM. A., NazifiM. I., UsmanM. B., & IbrahimU. M. (2024). ISOLATION OF SQUALENE FROM DICHLOROMETHANE EXTRACT OF CROTON NIGRITANUS LEAF. FUDMA JOURNAL OF SCIENCES, 8(2), 212 - 216.