ISOLATION OF SQUALENE FROM DICHLOROMETHANE EXTRACT OF CROTON NIGRITANUS LEAF
Abstract
A precursor of steroids with a variety of biological activities is squalene. The purpose of the study was to identify and describe the industrial/medical chemical found in Croton nigritanus. Using the solvents such as; dichloromethane, ethyl acetate, and n-hexane, the procedure entails a liquid-liquid extraction stage using the partition method and a solid-liquid extraction step using the maceration method. For separation and purification, gravity column chromatography (GCC) and thin-layer chromatography were employed. The isolated chemical's identity was determined using FTIR, 1H NMR and GC-MS spectroscopy. The inquiry successfully extracted and analyzed the major chemical compound which was identified as Squalene.
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