HAEMATOLOGICAL AND BIOCHEMICAL PROFILE OF Clarias gariepinus FROM OPI LAKE IN NSUKKA LOCAL GOVERNMENT AREA, ENUGU STATE
Abstract
Haematological and biochemical analysis have been recognized as helpful tools for monitoring fish health. The objective of the study was to investigate the reference value for the haematological and the biochemical profile of Clarias gariepinus from Opi Lake, Nsukka Local Government Area of Enugu State, Nigeria. A total of thirty samples of Clarias gariepinus comprising twelve males and eighteen females were obtained from the Lake. The blood samples were collected and analysed to obtain the haematological and biochemical parameters using standard methods. The results show that the haematological profile of Clarias gariepinus from Opi Lake were significantly different (p<0.05) between male and female. The result of the biochemical analysis was also influenced by sex, as the serum aspertate aminotranferase (AST) and alanine aminotransferase (ALT) were significantly higher in females than males. The results of this study provide useful information for comparative studies and also for water quality and fish health status monitoring.
References
Abalaka, S.E. (2013). Evaluation of the Haematology and Biochemistry of Clarias gariepinus as Biomakers of Environmental Pollution in Tiga dam, Nigeria. Brazilian Archives of Biology and Technology, 56(3):371-376. DOI: https://doi.org/10.1590/S1516-89132013000300004
Adebayo, O.T., Fagbenro, O.A., Ajayi, C.B. and Popoola, O.M. (2007). Normal haematological profile of Parachanna obscura as a diagnostic tool in aquaculture. International Journal of Zoological Research 3(4): 193–199. DOI: https://doi.org/10.3923/ijzr.2007.193.199
Babson, A. L., S. J. Greeley, S. C. M. Coleman, and G. E. Philips. (1984). “Phenolphtalein Monophosphate as a Substrate for Serum Phosphatase.” Clincal Chemistry 12 (18): 482–490. DOI: https://doi.org/10.1093/clinchem/12.8.482
Dacie, J.V. and Lewis, S.M. (2001). Practical Haematology. 9th Edition. Churchill Livingstone, London, 633Pp.
Fazio F, Faggio C, Marafioti S, Torre A, Sanfilippo M, Piccione G. (2012). Comparative study of haematological profile on Gobius niger in two different habitat sites: Faro Lake and Tyrrhenian Sea. Cahiers De Biologie Marine 53:213–219.
Gabriel, U.U., Ezeri, G.N.O. and Opabunmi, O.O. (2004). Influence of sex, source, health status and acclimation on the haematology of Clarias gariepinus (Burch, 1822). African Journal of Biotechnology 3:463–467. DOI:10.5897/AJB. DOI: https://doi.org/10.5897/AJB2004.000-2090
Goel, K.A., Mishra, B.P., Gupta, K. and Wadhwa, S. (1984). A comparative Haematological study on a few freshwater Teleost. Indian J. Fisheries. 31(1):108-112.
Hare, L. and Carter J.C.H. (1987). The benthos of a natural West African lake, with emphasis on the diet migrations and Lumar and seasonal penodicities of the chaoboams (Diptera, chaoboidae). Freshwater Biology. 16,759–780. DOI: https://doi.org/10.1111/j.1365-2427.1986.tb01016.x
Ebeh, H. N., Ogbonna, S. A. and Nwani, C.D. (2016): Plasma biochemistry, haematological and biometric reference values of Protopterus annectens of Anambra River, Nigeria, Zoology and Ecology. DOI: https://doi.org/10.1080/21658005.2016.1268385
Hrubec, T.C., Cardinale, J.L. and Smith, S.A. (2000). Haematology and Plasma Chemistry Reference Intervals for Cultured Tilapia (Oreochromis Hybrid). Veterinary Clinical Pathology 29(1): 7 –12. DOI: https://doi.org/10.1111/j.1939-165X.2000.tb00389.x
Inyang, N. M. (1995). The fish fauna of Opi lake, South eastern Nigeria, with particular reference to the Biology of Tilapia zilli. Journal of Aquacultural Science. 10, 29–36. Journal for the Tropics. 12(1):579-582.
Jawad, L.A., Al–Mukhtar, M.A. and Ahmed, H.K. (2004). The relationship between haematocrit and some biological parameters of the Indian shad, Tenualosa ilisha (Family Clupeidae). Animal Biodiversity Conservation 27: 478–483.
Kaleem, O. and Sabi, A.-F.B.S. (2021) .Overview of aquaculture systems in Egypt and Nigeria, prospects, potentials, and constraints. Aquac. Fish. 6, 535–547. DOI: https://doi.org/10.1016/j.aaf.2020.07.017
Magnadottir B. (2006). Innate immunity of fish (overview). Fish and Shellfish Immunology 20:137–151. DOI:10.1016/j. fsi.2004.09.006. DOI: https://doi.org/10.1016/j.fsi.2004.09.006
Melefa, T.D., Bernard O. Mgbenka, Ifeanyi O. Aguzie, Felix A. Andong, Uju Nwakor, Nwani, C.D., (2020). Morphological, haematological and biochemical changes in African catfish Clarias gariepinus (Burchell 1822) juveniles exposed to clotrimazole Comparative Biochemistry and Physiology, Part C 236, 108815. DOI: https://doi.org/10.1016/j.cbpc.2020.108815
Newman, S. H., Piatt, J. F. and White, J. (1997). Haematological and plasma biochemical reference ranges of Alaskan seabirds: their ecological significance and clinical importance. Waterbirds 20(3): 492–504. DOI: https://doi.org/10.2307/1521600
Nwani, C. D., O. P. Somdare, J. A. Ukonze, V. C. Ejere, A. O. Nwadinigwe, J. C. Nwani, G. E. Odo, and O. N. Ugbor. 2016. “Subchronic Exposure to Fenthion Induce Haematological Changes in African Catfish Clarias gariepinus (Burchell 1822).”Journal of Aquatic Animal Health 28 (4): 229–234. DOI: https://doi.org/10.1080/08997659.2016.1194907
Odo, G. E., Nwamba, H. O. and Eyo, J. E. (2009). Aspect of the Biology of Heterotis niloticus Cuvier 1829 (Osteoglossiformes: Osteoglossidae) in the Anambra flood River system, Nigeria. Animal Research International 6(2): 994 – 1002. DOI: https://doi.org/10.4314/ari.v6i2.48131
Ogunji, J and Wuertz, S. (2023). Aquaculture Development in Nigeria: The Second Biggest Aquaculture Producer in Africa. Water.15(24):4224. https://doi.org/10.3390/w15244224. DOI: https://doi.org/10.3390/w15244224
Okafor, A. I., and L. O. Chukwu. (2010). Haematological Profile of the African Lungfish, Protopterus annectens (Owen) of Anambra River Nigeria. Journal of American Science 6 (2): 123–130.
Onyia, L.U., Diyaware, M.Y., Michael, K.G., Musa, M. and Ochokwu, I. J. (2015). Comparison of Haematological Indices, Blood Group and Genotype of Clarias gariepinus (Burchell, 1822)and Clarias anguillaris (Linneaus, 1758). Journal of Fisheries and Aquatic Science 10(5): 392– 399. DOI: https://doi.org/10.3923/jfas.2015.392.399
Parrino, V., T. Cappello, G. Costa, C. Cannavà, M. Sanfilippo, F. Fazio and S. Fasulo (2018) Comparative study of haematology of two teleost fish (Mugilcephalus and Carassius auratus) from different environments and feeding habits, The European Zoological Journal, 85:1, 193-199, DOI: 10.1080/24750263.2018.1460694. DOI: https://doi.org/10.1080/24750263.2018.1460694
Pollard, S., Anderson, J.C., Bah, F., Mateus, M., Sidhu, M. and Simmons, D. (2022). Non-Lethal Blood Sampling of Fish in the lab and Field With Methods for Dried Blood Plasma Spot Omic Analyses. Front Genet. Mar 24;13:795348. doi: 10.3389/fgene.2022.795348. PMID: 35401689; PMCID: PMC8988233. DOI: https://doi.org/10.3389/fgene.2022.795348
Reitman, S., and S. Frankel. (1957). Colorimetric Method for the Determination of Serum Oxaloacetic and Glutamine Pyruvic Transaminases. American Journal of Clinical Pathology 766:28–56. DOI: https://doi.org/10.1093/ajcp/28.1.56
Romano, N., Scapigliati, G. and Abelli L. (2017). Water oxygen content affects distribution of T and B lymphocytes in lymphoid tissues of farmed sea bass (Dicentrarchus labrax). Fishes 2:16. DOI:10.3390/fishes2030016. DOI: https://doi.org/10.3390/fishes2030016
Satheeshkumar, P., Ananthan, G., Senthil Kumar, D. and Jagadeesan, L. (2011). Haematology and biochemical parameters of different feeding behaviour of teleost fishes from Vellar estuary, India. Comp Clin Pathol DOI 10.1007/s00580-011-1259-7. DOI: https://doi.org/10.1007/s00580-011-1259-7
Seibel, H., Baßmann, B. and Rebl, A. (2021). Blood Will Tell: What Hematological Analyses Can Reveal About Fish Welfare. Front Vet Sci. Mar 30;8:616955. doi: 10.3389/fvets.2021.616955. PMID: 33860003; PMCID: PMC8042153. DOI: https://doi.org/10.3389/fvets.2021.616955
Sood, R. (2006). Textbook of Medical Laboratory Technology. New Delhi: Jaypee Brothers Medical. DOI: https://doi.org/10.5005/jp/books/10919
Srivastava S, Choudhary SK. (2010). Effect of artificial photoperiod on the blood cell indices of the catfish, Clarias batrachus. Journal of Stress Physiology and Biochemistry 6:22–32.
Svobodova, Z., Pravola, D. and Palackova, J. (1991). Unified methods of haematological examination of fish. Research Inst. of fish culture and Hydrobiology. Vodnany, Czechoslovakia., 31Pp.
Truter, M., Hadfield, K.A. and Smit, N.J. (2023). Review of the metazoan parasites of the economically and ecologically important African sharptooth catfish Clarias gariepinus in Africa: Current status and novel records. Adv Parasitol.;119:65-222. doi: 10.1016/bs.apar.2022.11.001. Epub 2023 Jan 20. PMID: 36707175. DOI: https://doi.org/10.1016/bs.apar.2022.11.001
Wedemeyer, G.A. and Yasutake, W.T. (1977). Clinical methods for the assessment of the effect of environmental stress on fish health. Technical paper at the US fish and wildlife service. No.89. Washington, DC, US Dept. of interior, Fish and wildlife service, 180Pp.
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