• Ibrahim Doma Abdullahi Department of Pharmacology Bayero Univesity Kano
  • Mustapha Ibrahim Gudaji Faculty of Clinical Sciences, Bayero University Kano
  • Abdullahi Hamza Yaro Department of Pharmacology and Therapeutics, Bayero University, Kano, Nigeria.
Keywords: Antimalarial, Cytokines, Ficus asperifolia, Plasmodium berghei, RAF

Author Biographies

Mustapha Ibrahim Gudaji, Faculty of Clinical Sciences, Bayero University Kano

Former HOD, Department of Psychiatry

Abdullahi Hamza Yaro, Department of Pharmacology and Therapeutics, Bayero University, Kano, Nigeria.

HOD Department of Pharmacology and Therapeutics, Bayero University, Kano, Nigeria.


Abdullahi, I. D., Umar H. Y., Suleiman U, B., Abdullahi R. A., Maitama A. Y., Abdullahi R. A and Yaro, A. H. (2023). Quantitative Determination of Phytochemical Constituents of Fractions Obtained from Ficus asperifolia Leaves Miq (Moraceae) and the Characterization of Compounds Identified in the Residual Aqueous Fraction; FUDMA Journal of Sciences Vol. 7 No.2, 330-343. DOI:

Abdullahi, I. D., Yaro, A. H and Nazifi, A. B. (2020). Anti-nociceptive and Anti-inflammatory Activities of Solvent Fractions of Methanol Leaf Extract of Ficus asperifolia miq. (Moraceae) in Murine Models; Nigerian Journal of Pharmaceutical Sciences Vol. 19 No.2, 25-37.

Adebayo S. A., Dzoyem J. P., Shai L. J and Eloff J. N. (2015). The Anti-inflammatory and Antioxidant Activity of 25 Plant Species used Traditionally to Treat Pain in Southern African. BMC Complimentary Alternative Medicine; 15:1-10. DOI:

Afolabi B. A., Obafemi T. O., Akinola T. T., Adeyemi A.S and Afolabi O. B. (2014). Effect of Ethanolic Extract of Alstonia boonei Leaves on Serum Electrolyte Levels in Wistar Albino Rats. Phol-Pharmacology Online; 3: 85-90.

Afolayan, F.I.D., Olayemi Adegbolagun , Nicholas N. Mwikwabe , Jennifer Orwa and Chiaka Anumudu (2020). Cytokine Modulation during Malaria Infections by some Medicinal plants; Scientific African; 8 (2020) e00428. DOI:

Akanbi O. M., Elekofehinti O., Olatokunbo A., Adejuyigbe A and Jegede A. (2018). Anti-malarial Activity of Total Saponins from Terminalia avicennioides and Its Effect on Liver and Haematological of Infected Mice. Drug Designing; 7: 2. DOI: 10.4172/2169-0138.1000161.

Akuodor, G.C., Anyalewech, N.A., Ikoro, N.C., Akpan, J.L., Megwas, U.A., Iwuanyanwu, T.C. (2010). Evaluation of Antiplasmodial Activity of Berlina grandiflora Leaf Extract Against Plasmodium berghei in Mice. African Journal of Microbiological Research; 4(21): 2211-2214.

Alshawsh, S.M., Mothana, R.A., Al-Shamaly, H.A., Alsllami, S.F. and Lindequist, U. (2007). Assessment of Antimalarial Activity Against Plasmodium falciparum and Phytochemical Screening of Some Yemeni Medicinal Plants. Ecam; 6:453-456. DOI:

Artavanis-Tsakonas K., Tongren J.E and Riley E.M. (2003). The war between the malaria parasite and the immune system: immunity, immunoregulation and immunopathology. Clinical Experimental Immunology; 133: 145-152. DOI:

Asanga Edet E., Eseyin Olorunfemi, Ebong Patrick, Igile Godwin,Thomas Paul S and Ebong A. (2017). Antiplasmodial Activity of Ethanol Extract and Fractions of Nauclea Latifolia Smith (Rubiacea) Roots. World J Pharm Sci ; 5(5): 106-118.

Awasthi A, Kumar A, Upadhyay SN, Yamada T, Matsunaga Y. 2003. Nitric oxide protects against chloroquine resistant Plasmodium yoeli nigeriensis parasites in vitro. Experimental Parasitology; 105: 184-191. DOI:

Boampong J. N et al. (2013). The Curative and Prophylactic Effects of Xylopic acid on Plasmodium berghei infection in mice. Journal of Parasitology Research; http// DOI:

Burkill, H.M. (1997). The Useful Plants of West Tropical Africa. Vol. 4 2nd edition. Royal Botanic Gardens, Kew, Richmond, Survey TW 8 3AE Pp 293.

Chen, L., Huidan Deng, Hengmin Cui, Jing Fang, Junliang Deng ZhicaiZuo, Yinglun Li, Xun Wang and Ling Zhao (2018). Inflammatory Responses and Inflammation- associated diseases in organs; Oncotarget 9 (6): 7204–7218. DOI:

Crompton P.D., J. Moebius, S. Portugal , M. Weisberg , G. Hart , L.S. Garver , L.H. Miller , C. Barillas-Mury and S.K. Pierce (2014). Malaria Immunity in Man and Mosquito: Insights into Unsolved Mysteries of a Deadly Infectious Disease. Annual Revision Immunology; 32: 157–187. DOI:

Day NP, Hien TT, Schollaardt T, Loc PP, Chuong LV et al. (1999). The prognostic and pathophysiologic role of pro- and antiinflammatory cytokines in severe malaria. Journal of Infectious Diseases; 180:1288-1297. DOI:

Dunst J., F. Kamena and Matuschewski K. (2017). Cytokines and Chemokines in Cerebral Malaria Pathogenesis, Front. Cell and Infectious Microbiology; 7: 324. DOI:

Edewor-Kuponiyi, T. I. (2013). Plant-Derved Compounds with Potential Sedative and Anxiolytic Activities. International Journal of Basic and Applied Sciences; 2(1): 63-78. DOI:

Faustino, T.T., Almeida, R.B. and Andreatini, R. (2010). Medicinal Plants for the Treatment of Generalized Anxiety Disorder: A Review of Controlled Clinical Studies. Revista Brasileira de Psiquiatria, 32: 429-436 DOI:

Fidock D. A et al. (2004). Antimalarial Drug Discovery: Efficacy Models for Compound Screening. Nat Rev Drug Discovery; 3: 509 – 20. DOI:

Gebrehiwot , S. Shumbahri , M. Eyado , A and Yohannes T. (2019). Phytochemical Screening and In Vivo Antimalarial Activity of Two Traditionally Used Medicinal Plants of Afar Region, Ethiopia, against Plasmodium berghei in Swiss Albino Mice. Journal of Parasitology; Res.2019. DOI:

Heddini A. (2002). Malaria Pathogenesis: A Jigsaw with an Increasing number of Pieces. International Journal of Parasitology; 32: 1587-1598. DOI:

Hill D. R et al. (2006). “Primaquine: Report from CDC Expert meeting on Malaria Chemophylaxis I”. The American Journal of Tropical Medicine and Hygiene; 75 (3): 402 – 15. DOI:

Imafidon, K. E., Abu, O. D., Obayuwana, H. O and Okuofu, E. D. (2018). Phytochemical, Proximate and Metal content Analysis of Citrullus lanatus (Watermellon) Seed; FUDMA Journal of Sciences – Vol. 2 No. 2

Khan, M.E., Amupitan, J.O., Oyewale, A.O. and Ndukwe, I.G (2015). Evaluation of the In vivo Antimalarial Activity of the Methanolic Leaf Extract of Nepata cateria; Research in Pharmaceutical Biotechnology Vol. 6(2), pp. 8-15. DOI:

Kirby GC. (1997). Plants as source of antimalarial drugs. Tropical Doc; 27 (1): 7 – 11. DOI:

Lawal, O.A., Adebayo, M.A., Sikiru, A.A. and Ogunwande, I.A. (2016). Chemical Composition and Antimicrobial Activity of Essential Oils of Ficus asperifolia Miq. and Ficus capensis Thunb From Nigeria. Journal of Essential Oil Bearing Plants; 19: 1693- 1700. DOI:

Maikai, V.A., Kobo, P.I. and Adaudi, A.O. (2008). Acute Toxicity Studies of Aqueous Stem Bark Extract of Ximenia americana. African Journal of Biotechnology; 7(10): 1600-1603.

Malaguarnera L and Musumeci S. (2002).The immune response to Plasmodium falciparum malaria. Lancet Infectious Disiseases; 2: 472-478. DOI:

Matur, B.M., Mathew, T. and Ifeanyi, C.I.C (2009). Analysis of the Phytochemical and In-vivo Antimalarial Properties of Phyllanthus fraternus Webster Extract. New York Science Journal; 2:12-19.

Mengiste, B., Makonnen, E. and Urga, K (2012). Invivo Antimalarial Activity of Dodonaea angustifolia Seed Extracts against Plasmodium berghei in Mice Model. Momona Ethiopian Journal of Science; 4(1): 47-63. DOI:

Munzer, Robert A., and Benjamin F. Weiner. (1980). "Blood Sedimentation Rate Test Means." U.S. Patent No. 4,197,735. 15 Apr. 1980.

Nafiu M. O., Ashafa A. O. T., Adewuyi A. I and·Abdulsalam T. A. (2021). Advances in Traditional Medicine; Springer: 13596-021-00571-w

Nergiz C and Otles S. (1993). Chemical Composition of Nigella sativa I. Seeds. Food Chem; 48: 259 – 61. DOI:

Nkafamiya I.I., Osemeahon, S.A., Modibbo, U.U. and Aminu, A. (2010). Nutritional Status of Non-conventional Leafy Vegetables, Ficus asperifolia and Ficus sycomorus. African Journal of Food Science; 4(3): 104-108.

Nwanko, I.U. and Ukaegbu-Obi, K.M. (2014). Preliminary Phytochemical Screening and Antibacterial Activity of two Nigerian Medicinal Plants ( Ficus asperifolia and Terminalis catappa). Journal of Medicinal Plant and Herbal Therapy Research, 2: 1-5.

Ojo, A.O. and Akintayo, C.O. (2014). Assessment of Antioxidant Activity of Ficus asperifolia Miq Aqueous Extract –Invitro Studies. TheJjournal of Phytopharmacology, 3(1):16-21. DOI:

Okokon JE, Nwafor PA. (2009). Anti-plasmodial activity of root extract and fractions of Croton zambesicus. Journal of Ethnopharmacology; 121:74–8. DOI:

Omoniwa, B.P., Luka, C.D. and Soji-Omoniwa, O. (2013). Effect of Aqueous Leaf Extract of Ficus asperifolia on Cardiac Enzymes and Lipid Profile in Male Albino Rats. Journal of Medical Sciences; 13: 373-378. DOI:

Omoniwa, B.P. and Luka, C.D. (2012). Antidiabetic and Toxicity Evaluation of Aqueous Stem Extract of Ficus asperifolia in Normal and Alloxan-induced Diabetic Albino Rats. Asian Journal Experimental Biological sciences; 3:726-732.

Organization for Economic Co-operation and Development (OECD). (2001). Guidelines for Testing Chemicals: Acute Oral Toxicity e Fixed Dose Prpcedure (No .420), Section 4, OECD Publishing, Paris, France: pp1e14.

Peters W (1967). Rational methods in the search for anti-malaria drugs. Trans. R. Soc. Tropical Medicine Hygiene; 61:400-410. DOI:

Peters W., Portus H and Robinson L. (1975). The four-day suppressive in vivo antimalarial test. Ann Tropical Medical Parasitology; 69:155–71. DOI:

Philip F. U. (2020). Alkaloids from Plants with Antimalarial Activity: A Review of Recent Studies; Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 8749083, p 1-17.

Raji, Y., Oyeyemi, W.A., Shittu, S.T. and Bolarinwa, A.F. (2011). Gastro-Protective Effect of Methanol Extract of Ficus asperifolia Bark on Indomethacin Induced Gastric Ulcer in Rats. Nigerian Journal of Physiological Sciences; 26: 43-48.

Rungsung, W., Ratha, K.K., Dutta, S., Dixit, A.K and Hazra, J. (2015). Secondary Metabolites of Plants in Drugs Discovery. World Journal of Pharmaceutical Research; 4(7): 604-613.

Ryley JF, Peters W (1970). The Anti-malaria Activity of some Quinolone esters. AmericanTropical Medical Parasitology; 84:209-222. DOI:

Seo, E., Efferth, T. and Panossian, A. (2018). Curcumin Down Regulates Expression of OpioidRelated Nociceptin Receptor Gene (OPRL1) in Isolated Neuroglia Cells. Phytomedicine; 50: 285-299. DOI:

Shabbir, M., Muhammad, R. K. and Naima, S. (2013). Assessment of Phytochemicals, Antioxidant, Anti-lipid Peroxidation and Antihemolytic Activity of Extract and 81 various Fractions of Mytenus royleanus. BMC Complementary and Alternative Medicine. 13:143-145. DOI:

Tajuddeen N., Swart T., Hoppe H.C and Heerden, F.R. (2021). Antiplasmodial and Cytotoxic Flavonoids from Pappea capensis (Eckl. & Zeyh.) Leaves. Molecules; 26: 3875. DOI:

Toma A, Deyno S, Fikru A, Eyado A, Beale A. (2015). In vivo Antiplasmodial and Toxicological Effect of Ethanol Extract of Echinops kebericho Traditionally used in Treatment of Malaria in Ethiopia. Malaria. 2015; 14(1):196. DOI:

Udobre A. S et al. (2013). Effect of Methanol Leaf Extract of Nauclea latifolia on Albino Mice Infected with Plasmodium berghei berghei. African Journal of Pharmacology and Therapeutics; 2 (3): 83 – 87.

Watcho, P., Ngadjui, E., Alango, N.P., Benoit, N.T. and Kamanyi, A. (2009). Reproductive Effects of Ficus asperifolia (Moraceae) in Female Rats. African Health Sciences; 9: 49-53.

White NJ. (2008). Quighaosu (artemisin): The Price of Success. Science; 320: 330 – 4. DOI:

Yahfoufi, N. Alsadi, N. Jambi, M and Matar, C. (2018). The Immunomodulatory and Anti-Inflammatory Role of Polyphenols. Review. Nutrients; 10: 1618, Doi: 10.3390/nu10111618.

Yoo HJ et al. (2008). Anti-angiogenic Antinociceptive and Anti-inflammatory Activities of Lonicera japonica Extract. Journal of Pharmacy and Pharmacology; 60: 779 – 86 DOI:

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