MOLECULAR CHARACTERIZATION OF DENGUE VIRUS AND VECTORIAL COMPETENCE OF AEDES MOSQUITOES: A LOCAL MODEL OF XENOMONITORING
Abstract
Dengue is a major public health concern throughout tropical and sub-tropical regions of the world. Dengue virus is transmitted by infected female mosquitoes mainly of the species of Aedes aegypti and Ae. albopictus.Vector of Aedes mosquito populations may present different levels of competence for arbovirus transmission.Therefore, this study aimed at detecting DENV serotypes circulating and vectorial competence of adult field collected Aedes populations from selected local government areas of Kaduna State, Nigeria. A total of 233 mosquitoes were collected, identified morphologically to species level using coloured identification keys of Rueda and dissected under a dissecting microscope each species identified was tested in a pools of 20 individuals. Their bodies and head/salivary glands were separated and transferred to separate microtubes. RNA extraction was done using viral RNA extraction kit and semi-nested reverse transcription-polymerase chain reaction (RT-PCR) was used to amplified for DENV serotypes in the head/salivary glands of the Aedes mosquitoes. The study morphologically identified Ae. aegypti, Ae. vittatus and Ae. Furcifer. Aedes aegypti is the most abundant species 77.3%(180/233) while Ae. furcifer 10.3% (24/233) were the least. DENV serotypes were detected in 76.9%(10/13) of the pools of Aedes mosquitoes in the study areas. DENV1-3 serotypes were detected in Ae. Aegypti, DENV2 was detected in Ae. vittatus and DENV 2 and 3 were detected in Ae. Furcifer. Dengue virus 2 was the most detected serotypes in the study areas. PCR pools screening infectivity prevalence were found to be 7.07% (95% CI- 3.38-11.77). Thus, the finding...
References
Abubakar, A. (2014). Studies on the abundance and distribution of Mosquitoes and prevalence of Malaria in Yabo Local Government Area of Sokoto State, Nigeria. Unpublished MSc. Dissertation. Submitted to Usmanu Danfodiyo University, Sokoto.
Adeleke, M. A. (2008). Mosquito Species breeding in Artificial Containers in Ikenne Farm Settlement, Ogun State, Nigeria. BSc. Dissertation submitted to the University of Agriculture, Abeokuta, pp35.
Aigbodion, F. I., and Uyi, O. O. (2013). Temporal distribution of and Habitat diversification by some Mosquitoes (Diptera: Culicidae) species in Benin City, Nigeria. Journal of Entomology, 10(1), 13-23. DOI: https://doi.org/10.3923/je.2013.13.23
Ayukekbong, J. A. (2014). Dengue virus in Nigeria: current status and future perspective. Hosts and Viruses, 1(4), 106.
Baba, M. M., and Talle, M. (2011). The effect of climate on dengue virus infections in Nigeria. New York Science Journal, 4(1), 28-33.
Baba, M.M., Marie-Francois, S., Vorndam, A.V., Adeniji, A.O., Diop, O. and Olaleye, D. (2009). Dengue virus infection in patients suspected of malaria/typhoid in Nigeria. Journal of American Science, 5(5): 129 – 134.
Balingit, J. C., Carvajal, T. M., Saito-Obata, M., Gamboa, M., Nicolasora, A. D., Sy, A. K., ... & Watanabe, K. (2020). Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines. Parasites & vectors, 13(1):1-13. DOI: https://doi.org/10.1186/s13071-020-04470-y
Bhatt, S., Gething, P. W., Brady, O. J., Messina, J. P., Farlow, A. W., Moyes, C. L., and Hay, S. I. (2013). The global distribution and burden of dengue. Nature, 496 (7446), 504-507. DOI: https://doi.org/10.1038/nature12060
Boete, C., and Koella, J. C. (2003). Evolutionary ideas about genetically manipulated mosquitoes and malaria control. TRENDS in Parasitology, 19(1), 32-38. DOI: https://doi.org/10.1016/S1471-4922(02)00003-X
Bonizzoni, M., Gasperi, G., Chen, X., and James, A. A. (2013). The Invasive Mosquito Species Aedes albopictus: current knowledge and future perspectives. Trends in Parasitology, 29(9):460-468. DOI: https://doi.org/10.1016/j.pt.2013.07.003
Bosio, C.F, Harrington, L.C, Jones, J.W., Sithiprasasna, R., Norris, D.E., & Scott, T.W. (2005). Genetic structure of Aedes aegypti populations in Thailand using mitochondrial DNA. American Journal of Tropical Medicine Hygiene, 72:434 - 442. DOI: https://doi.org/10.4269/ajtmh.2005.72.434
Bustamante, D. M., & Lord, C. C. (2010). Sources of error in the estimation of mosquito infection rates used to assess risk of arbovirus transmission. The American journal of tropical medicine and hygiene, 82(6), 1172. DOI: https://doi.org/10.4269/ajtmh.2010.09-0323
Chimaeze, C.O., Chukwuemeka, N.A., and Okechukwu, & N.E. (2018). Diversity and distribution of Aedes Mosquitoes in Nigeria. NY Sci J, 11(2), 50-57.
Choy, M. M., Ng, D. H., Siriphanitchakorn, T., Ng, W. C., Sundstrom, K. B., Tan, H. C., ... and Ooi, E. E. (2020). A non-structural 1 protein G53D substitution attenuates a clinically tested live dengue vaccine. Cell Reports, 31(6):107617. DOI: https://doi.org/10.1016/j.celrep.2020.107617
Christie, J. (1872). Remarks on “Kidinga Pepo”: a Peculiar form of exanthematous disease. British medical journal, 1(596):577. DOI: https://doi.org/10.1136/bmj.1.596.577
Chukwuekezie, U. C., Nwangwu, O. C., Ogudu, E. O., Okoronkwo, A. I., Okoye, C. K., Onuora, E. N., ... and Igu, P. O. (2018). A Cross sectional survey of yellow fever and dengue virus vectors in four communities of Ayamelum Local Government Area (LGA), Anambra State, Southeast Nigeria. New York Science Journals, 9(3).
Dawurung, J.S., Baba, M.M., Stephen, G., Jonas, S.C., Bukbuk, D.N., Dawurung, C.J. (2010). Serological evidence of acute dengue virus infection among febrile patients attending Plateau State Specialist Hospital Jos, Nigeria.
Díaz, Y., Chen-Germán, M., Quiroz, E., Carrera, J. P., Cisneros, J., Moreno, B., ... and López-Vergès, S. (2019). Molecular epidemiology of dengue in Panama: 25 years of circulation. Viruses, 11(8), 764. DOI: https://doi.org/10.3390/v11080764
Emeribe, A. U., Abdullahi, I. N., Isong, I. K., Emeribe, A. O., Nwofe, J. O., Shuaib, B. I., ... and Oyewusi, S. (2021). Dengue virus is hyperendemic in Nigeria from 2009 to 2020: a contemporary systematic review. Infection and Chemotherapy, 53(2), 284. DOI: https://doi.org/10.3947/ic.2020.0142
Fahri, S., Yohan, B., Trimarsanto, H., Sayono, S., Hadisaputro, S., Dharmana, E., ... and Sasmono, R. T. (2013). Molecular surveillance of dengue in Semarang, Indonesia revealed the circulation of an old genotype of dengue virus serotype-1. PLoS neglected tropical diseases, 7(8):e2354. DOI: https://doi.org/10.1371/journal.pntd.0002354
Gaye, A., Wang, E., Vasilakis, N., Guzman, H., Diallo, D., Talla, C. and Diallo, M. (2019). Potential for sylvatic and urban Aedes mosquitoes from Senegal to transmit the new emerging dengue serotypes 1, 3 and 4 in West Africa. PLoS neglected tropical diseases, 13(2), e0007043. DOI: https://doi.org/10.1371/journal.pntd.0007043
Gu, W., & Novak, R. J. (2004). Detection probability of arbovirus infection in mosquito populations. The American journal of tropical medicine and hygiene, 71(5):636-638. DOI: https://doi.org/10.4269/ajtmh.2004.71.636
Gubler, D. J. (1997). Dengue and dengue hemorrhagic fever. In Seminars in Pediatric Infectious Diseases. WB Saunders. 8(1): 3-9. DOI: https://doi.org/10.1016/S1045-1870(97)80003-9
Gubler, D.J. (2011). Dengue, urbanization and globalization: the unholy trinity of the 21st century. Tropical medicine and health, 39:3-11. DOI: https://doi.org/10.2149/tmh.2011-S05
Guedes, D. R. D., Cordeiro, M. T., Melo-Santos, M. A. V., Magalhaes, T., Marques, E., Regis, L., ... & Ayres, C.F.J. (2010). Patient-based dengue virus surveillance in Aedes aegypti from Recife, Brazil.
Guzmán, M. G., and Kouri, G. (2002). Dengue: an update. The Lancet infectious diseases, 2(1):33-42. DOI: https://doi.org/10.1016/S1473-3099(01)00171-2
Hakami, A. M., Mohammed, I., Qadri, M. I., Al-Ghamdi, K., Alkenani, N. A., Eisa, Z. M., ... & Bakri, M. (2018). Molecular identification and characterization of different dengue virus serotypes reported In Jazan Area, Kingdom of Saudi Arabia. Journal Experimental Biology and Agricultural Sciences, 6(5):828-835. DOI: https://doi.org/10.18006/2018.6(5).828.835
Harapan, H., Michie, A., Yohan, B., Shu, P.Y., Mudatsir, M., Sasmono, R.T., and Imrie, A. (2019). Dengue viruses circulating in Indonesia: A systematic review and phylogenetic analysis of data from five decades. Reviews in medical virology, 29(4): e2037. DOI: https://doi.org/10.1002/rmv.2037
Heilig, G. K. (2012). World urbanization prospects: the 2011 revision. United Nations, Department of Economic and Social Affairs (DESA), Population Division, Population Estimates and Projections Section, New York, 14, 555.
Hu, T. S., Zhang, H. L., Feng, Y., Fan, J. H., Tang, T., Liu, Y. H., ... and Fan, Q. S. (2017). Epidemiological and molecular characteristics of emergent dengue virus in Yunnan Province near the China-Myanmar-Laos border, 2013–2015. BMC infectious diseases, 17(1):1-12. DOI: https://doi.org/10.1186/s12879-017-2401-1
Idoko, M. O., Ado, S. A., and Umoh, V. J. (2015). Prevalence of dengue virus and malaria in patients with febrile complaints in Kaduna Metropolis, Nigeria. British Microbiology Research Journal, 8(1):343-347. DOI: https://doi.org/10.9734/BMRJ/2015/15588
Idris, A. N., Baba, M. M., Thairu, Y., and Bamidele, O. (2013). Sero-prevalence of dengue type-3 Virus among patients with febrile illnesses attending a tertiary hospital in Maiduguri, Nigeria. International Journal of Medicine and Medical Sciences, 5(12), 560-563.
Johnson, B. W., Russell, B. J., and Lanciotti, R.S. (2005). Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. Journal of clinical microbiology, 43(10):4977-4983. DOI: https://doi.org/10.1128/JCM.43.10.4977-4983.2005
Limkittikul, K., Brett, J., and L'Azou, M. (2014). Epidemiological trends of dengue disease in Thailand (2000–2011): a systematic literature review. PLoS neglected tropical diseases, 8(11):e3241. DOI: https://doi.org/10.1371/journal.pntd.0003241
Lundkvist, Å. (2013). Dengue viruses–an overview. Infect. Ecol. Epidemiol., 3, 19839. DOI: https://doi.org/10.3402/iee.v3i0.19839
Méndez, F., Barreto, M., Arias, J. F., Rengifo, G., Munoz, J., Burbano, M. E., & Parra, B. (2006). Human and mosquito infections by dengue viruses during and after epidemics in a dengue–endemic region of Colombia. The American journal of tropical medicine and hygiene, 74(4):678-683. DOI: https://doi.org/10.4269/ajtmh.2006.74.678
Messina, J. P., Brady, O. J., Scott, T. W., Zou, C., Pigott, D. M., Duda, K. A., ... and Hay, S. I. (2014). Global spread of dengue virus types: mapping the 70 year history. Trends in microbiology, 22(3):138-146. DOI: https://doi.org/10.1016/j.tim.2013.12.011
Musa, I.J., Shehu, A. and Lukman, S. (2009).The distribution method and supply problems of tap water in urban Zaria area, Kaduna State.Electronic Journal Environmental Agricultural and Food Chemistry, 8 (4):294-300.
Okogun, G. R., Anosike, J. C., Okere, A., Nwoke, B., and Esekhegbe, A. (2003). Epidemiological implications of preferences of breeding sites of mosquito species in Midwestern Nigeria. Annals of Agricultural and Environmental Medicine, 10(2), 217-222.
Otu, A., Ebenso, B., Okuzu, O., and Osifo-Dawodu, E. (2016). Using a mHealth tutorial application to change knowledge and attitude of frontline health workers to Ebola virus disease in Nigeria: a before-and-after study. Human Resources for Health, 14(1): 1-9. DOI: https://doi.org/10.1186/s12960-016-0100-4
Pérez-Castro, R., Castellanos, J. E., Olano, V. A., Matiz, M. I., Jaramillo, J. F., Vargas, S. L., ... & Overgaard, H. J. (2016). Detection of all four dengue serotypes in Aedes aegypti female mosquitoes collected in a rural area in Colombia. Memorias do Instituto Oswaldo Cruz, 111:233-240. DOI: https://doi.org/10.1590/0074-02760150363
Phadungsombat, J., Lin, M. Y. C., Srimark, N., Yamanaka, A., Nakayama, E. E., Moolasart, V., ... and Uttayamakul, S. (2018). Emergence of genotype Cosmopolitan of dengue virus type 2 and genotype III of dengue virus type 3 in Thailand. PLoS One, 13(11): e0207220. DOI: https://doi.org/10.1371/journal.pone.0207220
Pimenta, P.F.P., Caroline, M. Gonçalves , Fabrício, F.M., Juliana, M.T. Bezerra , Bárbara A.C., Breno, M.S., Luciana, D.S., José, E.M.P., Jorge, R.A., Nágila, F.C.S. and Douglas, E. N. (2014). Distinct variation in vector competence among nine field populations of Aedes aegypti from a Brazilian dengue-endemic risk city. Parasites & Vectors, 7(1):320. DOI: https://doi.org/10.1186/1756-3305-7-320
Pompon, J., Manuel, M., Ng, G. K., Wong, B., Shan, C., Manokaran, G., ... and Garcia-Blanco, M. A. (2017). Dengue subgenomic flaviviral RNA disrupts immunity in mosquito salivary glands to increase virus transmission. PLoS pathogens, 13(7): e1006535. DOI: https://doi.org/10.1371/journal.ppat.1006535
Rather, I. A., Parray, H. A., Lone, J. B., Paek, W. K., Lim, J., Bajpai, V. K., and Park, Y. H. (2017). Prevention and control strategies to counter dengue virus infection. Frontiers in cellular and infection microbiology, 7:336. DOI: https://doi.org/10.3389/fcimb.2017.00336
Rogers, D. J., Suk, J. E., and Semenza, J. C. (2014). Using global maps to predict the risk of dengue in Europe. Acta tropica, 129:1-14. DOI: https://doi.org/10.1016/j.actatropica.2013.08.008
Rueda, L. M. (2004). Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with dengue virus transmission. Zootaxa, 589(1):1-60. DOI: https://doi.org/10.11646/zootaxa.589.1.1
Salvo, M. A., Aliota, M. T., Moncla, L. H., Velez, I. D., Trujillo, A. I., Friedrich, T. C., and Osorio, J. E. (2019). Tracking dengue virus type 1 genetic diversity during lineage replacement in an hyperendemic area in Colombia. Plos one, 14(3):e0212947. DOI: https://doi.org/10.1371/journal.pone.0212947
Service, M. W. (1974). Survey of the relative prevalence of potential yellow fever vectors in north-west Nigeria. Bulletin of the World Health Organization, 50(6):487.
Service, M. W. (1980). A Guide to Medical Entomology, Macmillan Press Ltd., pp. 29 – 36 DOI: https://doi.org/10.1007/978-1-349-16334-2
Tolle, M. A. (2009). Mosquito-borne diseases. Curriclum Problem Pediatric Adolescence.Health Care, 4:97–104 DOI: https://doi.org/10.1016/j.cppeds.2009.01.001
World Health Organization. (2012). Global strategy for dengue prevention and control 2012-2020.
Zambrano, L. I., Sevilla, C., Reyes-Garcia, S. Z., Sierra, M., Kafati, R., Rodriguez-Morales, A.J., and Mattar, S. (2012). Potential impacts of climate variability on dengue hemorrhagic fever in Honduras, 2010. Tropical Biomed,ical, 29(4):499-507.
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