CYTOTOXICITY, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF THE LEAVES AND STEM BARK EXTRACTS OF VITELLARIA PARADOXA

Authors

  • Nazifi Saleh Ibrahim
  • Murtala Muhammad Namadina
  • Abubakar Khalil Rabil
  • Maryam Yakubu Bala

Keywords:

Vitellariaparadoxa, cytotoxicity, antioxidant, antimicrobial

Abstract

The effective use of Vitellariaparadoxa in traditional medicine for treatment of various ailments including inflammation, fever, skin irritation, dermatitis, sunburn, rheumatism, diarrhea, stomach ache and ulcers are well established. This study focused on the cytotoxicity of the plant parts using Brine Shrimp Lethality Assay (BSLA), antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and antimicrobial activity against Bacillus cereus, Staphylococcus aureus, Kleibsiella pneumonia and Escherichia coli using disc diffusion and minimum inhibitory concentration methods. Result of cytotoxicity showed that the leaves crude extract (VPL 01) is more cytotoxic with LC50 15.17µg/mL followed by the methanol fraction of both the leaves (VPL 04) and the stem bark (VPS 04) with LC50 values 23.21 and 19.35µg/mL respectively. The result of the antioxidant assay obeys the Beer-Lambert law over the useful range with both the leaves and stem back of Vitellariaparadoxa showing great antioxidant potency in all fractions tested. Themethanolic fraction standout above other factions with IC50 of 9.64µg/mL and 6.50µg/mL for the leaves and stem bark fractions respectively followed by the moderately polar ethyl acetate fraction which recorded IC5016.96µg/mLand16.38µg/mL for leaves and stem back in comparison with standard ascorbic acid and BHT which recorded 4.77µg/mL and 9.18µg/mL respectively. The antimicrobial activity showed that the ethanol leaves extract had the maximum zone of inhibition against the entire test organisms, while the methanol fraction showed maximum inhibition against B. cereus (18±0.73 mm), S. aureus (19±1.41 mm) and K. pneumonia (19±0.03 mm) with MIC values of 62.5µg/mL for all the organisms respectively. These studies indicated  

References

Agagu, O.K., Fayose, E. A. and Petters, S. W. (1985). "Stratigraphy and sedimentation in the Senonian Anambra Basin of Eastern Nigeria", Journal of Mining and Geology, 22: 25–36.

Ahmed, S., Carpenter, P. J. (2003). Geophysical response of filled sinkholes, soil pipes and associated bedrock fractures in thinly mantled karst, east-central Illinois. Environmental Geology 44 (6), 705-716.

Ameh, E. G. (2013). Multivariate statistical analyses and enrichment of heavy metal contamination of soils around Okaba coal mine. American-Eurasian Journal of Agronomy, 6(1): 9-13.

Antonio-Carpio, R. G., Perez-Flores, M. A., Camargo-Guzman, D., Alanis-Alcantar, A. (2004). Use of resistivity measurements to detect urban caves in Mexico City and to assess the related hazard. Natural hazards and Earth System Sciences 4 (4), 541-547.

BGR, (2009). (Bundesanstalt fur Geowissenschaften und Rohstoffe- Federal Institute for Geosciences and Natural Resources), Reserve, Resources and Availability of Energy Resources-Annul Report 2009, BGR, Hannover, Germany, www.bgr.bund.de.

Burke, K. C. (1996). The African Plate. South African Journal of Geology, 99: 341-409.

C.I.A.B., Coal Industry Advisory Board (2008). Clean Coal Technologies: Accelerating Commercial and Policy Drivers for Deployment, CIAB, International Energy Agency, Paris.

Cardarelli E., Di Filippo G., Tuccinardi E., (2006) Electrical resistivity tomography to detect buried cavities in Rome: A case study. Near Surface Geophysics 4: 387–392.

Christine K. (2015). The Nigeria Coal Atlas Heinrich Böll Foundation, Berlin (Germany), Heinrich Böll Foundation, Nigeria and Friends of the Earth International, London, UK, p. 10.

Dahlin T. and Zhou B. (2004). A numerical comparison of 2D resistivity imaging with ten electrode arrays. Geophysical Prospecting, 2004; 52: 379-398.

Eshimiakhe, D., Raimi J., Larry B., Abubakar H., Matthew M. O., (2018). Mineshaft Imaging Using 2D Electrical Resistivity Tomography in a Kaolin Mining Site at Kankara in North Central Nigeria. Journal of Geology & Geophysics 7:5. DOI: 10.4172/2381-8719.1000447.

Johnson W. J. (2003) Case histories of DC resistivity measurements to map shallow coal mine workings. The Leading Edge, 22: 571-573.

Kogbe, C. A. (1989). The Cretaceous and Paleogene sediments of southern Nigeria. In: Kogbe, C.A. (Ed.), Geology of Nigeria. Elizabethan Publishing Company, Lagos, pp. 325e334.

Loke, M. H. (2005). RES2DINV. Repaid 2-D resistivity and IP inversion using the Least-Squares method. Manual. Geotomo Software, Penang, Malays

Published

2023-04-12

How to Cite

Ibrahim, N. S., Namadina, M. M., Rabil, A. K., & Bala, M. Y. (2023). CYTOTOXICITY, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF THE LEAVES AND STEM BARK EXTRACTS OF VITELLARIA PARADOXA. FUDMA JOURNAL OF SCIENCES, 3(4), 562 - 567. Retrieved from https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/1686