EFFECT OF SCHISTOSOMA HAEMATOBIUM INFECTION AND DRUG TREATMENT ON THE IMMUNE SYSTEM OF IMMUNOSUPPRESSED GUINEA PIGS
Abstract
Guinea pigs were immunosuppressed, before infection with S. haematobium. They were treated with different drug combination (Artequine, Artemether and Praziquantel), to ascertain their efficacy. The Guinea pigs were acclimatized to laboratory conditions for a week prior infection with 200 S. haematobium cercariae. Male and female guinea pigs weighing 2.5–6.5 kg were divided into four groups, 1- 4 (with 36-42 guinea pigs each). They were further divided into twelve subgroups of A - L with 3-4 guinea pigs each. The grouping 1-4 was done to monitor the effect of the drugs after 2, 7, 28 and 90 days post infection on the haematological parameter. Blood sample for Differential White Blood Cell (WBC) were collected using orbital technique from the retrobulbar plexus of the medial canthus of the eye of the guinea pigs, before analysis. Infection with S. haematobium (Before) decreased the level of lymphocyte when compared with normal control (NC) two days post infection. Except for treatment (After) with 75mg/kg Praziquantel PZQ and 100/200 mg/kg PZQ/ART, all other treatment regimen decreased the level of lymphocyte seven days post infection. Eosinophilia was observed after infection when compared with the NC, hence the mean level of eosinophil decreases after treatment (eosinopenia). Infection with S. haematobium increased the level of eosinophil, treatment with 75 and 300 ART and PZQ further increased the level of eosinophil seven days post infection. Infection with S. haematobium caused eosinophil in some of the animal and treatment further elevated the value of eosinophil
References
Archetti I., Tittarelli C., Cerioli M., Brivio R., Grilli G., Lavazza A. (2008). Serum chemistry and hematology values in commercial rabbits: preliminary data from industrial farms in northern Italy. In proc.: 9th World Rabbit Congress, 10-13 June, Verona, Italy, 1147-1151.
Betancourt-Alonso M.A., Orihuela A., Aguirre V., Vázquez R., Flores-Pérez I. (2011). Changes in behavioural and physiological parameters associated with Taenia pisiformis infection in rabbits (Oryctolagus cuniculus) that may improve early detection of sick rabbits. World Rabbit Sci., 19: 21-30. doi:10.4995/wrs.2011.801
Bennett, J.L., Day, T., Liang, F.T., Ismail, M., and Farghaly, A. (1997). The development of resistance to anthelmintics: a perspective with an emphasis on the antischistosomal drug praziquantel. Exp Parasitol 87: 260-267.
Cioli, D., L. Pica-Mattoccia, and S. Archer. (1995). Antischistosomal drugs: past, present . . . and future? Pharmacology Therapv, 68: 35-85.
Davis A, Biles JE, Urich AM, Dixon H (1981). Tolerance and efficacy of praziquantel in phase IIA and IIB therapeutic trials. Arzneimittel Forschung Res. 33:568–582
El-Bassiouni, E. A., Helmy, M. H., Saad, E. I., Kamel, M. A. El- Nabi, Abdel-Meguid, E., Hussein, H. S. E. (2007). Modulation of the antioxidant defence in different developmental stages of Schistosoma mansoni by praziquantel and artemether. British Journal of Biomedical Science, 64(4) :168-74
Eric, A., Ottesen, and Peter, F. W. (1979) Eosinophil following treatment of patient with schistosomiasis mansoni and bancroft” filariasis. The journal of infectious diseases, 139 (5)
Gryseels B, Stelma F.F, Talla I, Van Dam G.J., Polman K, Sow S, Diaw M, Sturrock RF, Doehring-Schwerdtfeger E, Kardorff R, Decam C, Niang M, Deelder AM, (1994). Epidemiology, immunology and chemotherapy of Schistosoma mansoni infections in a recently exposed community in Senegal. Tropical Geography Medicine, 46: 209–219.
Geerts, S. and Gryseels, B. (2000). Drug resistance in human helminths: current situation and lessons from livestock. Clinical Microbiology Review, 13: 207–222.
Guisse F., Polman K., Stelma F.F., Mbaye A., Talla I., Niang M., et al. (1997) Therapeutic evaluation of two different dose regimens of praziquantel in a recent Schistosoma mansoni focus in Northern Senegal. American Journal of Tropical Medicine and Hygiene, 56: 511-4.
Hotez, P.J. Alvarado, M. Basanez, M.G. Bolliger, I. Bourne, R. Boussinesq, M.et al. (2014). The global burden of disease study 2010: interpretation and implications for the neglected tropical diseases. PLoS Negl Trop Dis, 8, p. e2865
Liang, Y.S., Coles, G.C., Doenhoff, M.J., (2000). Detection of praziquantel resistance in schistosomes. Tropical Medicine International Health, 5: 72.
Manneck, T., Haggenmu, Y., Ller and Keiser. J. (2010). Morphological effects and tegumental alterations induced by mefloquine on schistosomula and adult flukes of Schistosoma mansoni. Parasitology, 137: 85–98
Okeke, O. C, Ubachukwu, P. O. Okafor, F.C., and Soyinka, V. O. (2011) Parasitology and histopathological effects of immunosuppression in guinea pigs experimentally infected with S. haematobium. Journal of Helminthology, 1-4
Rothenberg, M. E., and Hogan, S. P. (2006). The eosinophil. Annual Review on Immunology, 24:147–174.
Schalm, O.W., Jain, N.C., Carroll, E.J. (1975) Veterinary Haematology. 3rd edition Lea & Febiger Philadelphia, 129 –25.
Southgate, V.R., Rollinson, D., Tchuente, L.A., and Hagan, P. 2005 Towards control of schistosomiasis in sub-Saharan Africa. J Helminthol 79: 181-185.
Stelma, F.F., Talla, I., Sow S., Kongs, A., Niang, M., Polman, K., et al. (1995). Efficacy and side effects of praziquantel in an epidemic focus of Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene, 53: 167-170
Utzinger, J., N’Goran, E.K., N’Dri, A., Lengeler, C., Tanner, M. (2000a). Efficacy of praziquantel against Schistosoma mansoni with particular consideration for intensity of infection. Tropical Medicine International Health, 5: 771–778.
Utzinger, J. Chollet, J. Jiqing , Y., Jinyan, M, Tanner M., Shuhua, Xiao (2001) Effect of combined treatment with praziquantel and artemether on Schistosoma japonicum and Schistosoma mansoni in experimentally infected animas . Acta Tropica, 80: 9–18
World Health Organization (1999). Report of the WHO Informal Consultation on Schistosomiasis Control. World Health Organization, Geneva.
World Health Organization (2006). Preventive Chemotherapy in Human Helminthiasis: Coordinated Use of Anthelminthic Drugs in Control Interventions: aManual For Health Professionals and Programme Managers. WHO Press, Geneva.
World Health Organization (2017). Fact sheet Schistosomiasis. Updated January 2017 http://www.who.int/mediacentre/factsheets/fs115/en/. Accessed March, 2017
Xiao, S., Shen, B., Chollet, .J, Utzinger, J., and Tanner, M. (2001). Tegumental alterations in juvenile Schistosoma haematobium harboured in hamsters following artemether treatment. Parasitology International, 50(3): 175–183.
Yang, Y.Q., Xiao, S.H., Tanner, M., Utzinger, J., Chollet, J., Wu, J.D., and Guo, J. (2001). Histopathological changes in juvenile Schistosoma haematobium harboured in hamsters treated with artemether. Acta Tropica, 79: 135–141.
Van Nassauw, L., Toovey, S., Van Op den Bosch, J., Timmermans, J. P. and Vercruysse, J. (2008). Schistosomicidal activity of the antimalarial drug, mefloquine, in Schistosoma mansoni-infected mice. Travel Medicine and Infectious Diseases 6, 253–258
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