SLOW GROWTH IN VITRO CONSERVATION OF GROUNDNUT (Arachis hypogaea L.)

  • M. M. Abdulmalik
  • I. S. Usman
Keywords: in vitro conservation, slow growth, mannitol, sucrose, groundnut

Abstract

In vitro conservation techniques such as slow growth is now routinely used to compliment conventional conservation approaches. Slow growth procedures provide short to medium term storage options, and offer several advantages such as: free from genetic erosion and convenient for international distribution. The present study is aimed at establishing a slow growth in vitro conservation protocol for Nigerian groundnut varieties. The experiment was conducted with four groundnut varieties: SAMNUT 10, SAMNUT 21, SAMNUT 22 and SAMNUT 23, four conservation media: Murashige and Skoog (MS) media supplemented with different concentrations of sucrose (30, 20,15,10g/L) in combination with different concentrations of mannitol (10,15, 20g/L) and maintained for four different conservation periods: 3, 6, 9 and 12 months. The experiment was arranged as 4 x 4 x4 factorial experiment in a completely randomized design of three replicates. In vitro regenerated shoots of 2cm length were cultured on slow growth media. Results obtained from the study show that maximum survival (33.1%) was observed in SAMNUT 23. While MS medium supplemented with 10g/L sucrose and 20g/L mannitol effectively reduced shoot growth and produced the highest
survival (74%) after 3months conservation period. This medium could therefore be used for the medium-term conservation of the groundnut varieties.

 

References

Al-Abdallat, A.M. Shibli, R.A Akash, M.W. Rabbaa, M. and Al-Qudah, T. (2017). In Vitro Preservation of Transgenic Tomato (Solanum lycopersicum L.) Plants Overexpressing the Stress-Related SlAREB1 Transcription Factor. International Journal of Molecular Sciences, 18, 1477. doi:10.3390/ijms18071477 www.mdpi.com/journal/ijms

Charoensub, R. and Phansiri, S. (2004). In vitro Conservation of Rose Coloured Leadwort: Effect of Mannitol on Growth of Plantlets. Kasetsart Journal (Natural Science), 38: 97 – 102.

Engelmann, F. (2004). Plant cryopreservation: Progress and prospects. In Vitro Cell. Dev. Biol. Plant, 40: 427–433. http://dx.doi.org/10.1079/IVP2004541

Kochert, G. Halward, T. Branch, W.D. and Simpson, C.E. (1991). RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species. Theoretical and Applied Genetics, 81:565–570. http://dx.doi.org/10.1007/BF00226719

Gianní, S. and Sottile, F. (2015). In vitro storage of plum germplasm by slow growth. Hort. Sci. (Prague), 42 (2): 61–69.

Hassan, N.A. Stino, R.G. Gomaa A.H. and Al-Mousa R.N. (2014). In vitro Medium-Term Germplasm Conservation and Genetic Stability of Grape (Vitis vinifera L.) Journal of Horticultural Science & Ornamental Plants, 6 (1): 9-17.

Malaurie, B. Trouslot, M.F. Berthaud, J. Bousalem, M. Pinel, A. & Dubern, J. (1998). Medium- term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm. Electronic Journal of Biotechnology, 1: 102-117. http://dx.doi.org/10.2225/vol1- issue3-fulltext-2

Minoo, D. Nirmal Babu, K. and Peter, K.V. (2006). Conservation of Vanilla species, in vitro. Scientia Horticulturae, 110(2):175-180. http://dx.doi.org/10.1016/j.scienta.2006.07.00 3

Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x

Rodrigues, P.H.V. Arruda, F. and Forti, V.A. (2018). Slowgrowth in vitro conservation of Heliconia champneiana cv. Splash under different light spectra. Scientia Agricola, 7(2):163-166. doi: http://dx.doi.org/10.1590/1678-992X-2016-0394

SAS Institute Inc. (1990). SAS/STAT User's guide, version 64th edn. SAS Inc. Cary, NC, USA.

Sarkar, D. and Naik, P.S. (1998). Factors affecting minimal growth conservation of potato microplants in vitro. Euphytica, 102(2): 275-280. http://dx.doi.org/10.1023/A:1018309300121

Scherwinski-Pereira J.E., F.H.S. Costa, J. Camillo, D.B. Silva, R.B.N. Alves, and R.F. Vieira, (2010). Tissue culture storage of Brazilian medicinal plants germplasm. Acta Horticulturae, 860:211-214.

Shawky, B. and Aly, U.I. (2007). In vitro conservation of globe artichoke (Cyanara scolymus L.) germplasm. International Journal of Agriculture and Biology, 9(3): 404-407.

Shibli, R.A. Shatnawi, M.A. Subaih, W. and Ajlouni, M.M. (2006). In vitro conservation and cryoconservation of plant genetic resources: a review. World Journal of Agricultural Sciences, 2(4):372-382.

Tahtamouni, R.W. Shibli, R.A. and Ajlouni, M.M. (2001). Growth responses and physiological disorders in wild pear (Pyrus syriaca Boiss.) during slow-growth in vitro preservation on osmostressing media. Plant Tissue Culture, 11: 15–23.

Taiz, L. and Zeiger, E. (2003). Auxins. In: Plant Physiology. New York: Macmillan Publishing Co., pp. 544.

Wilkins, C.P. Dodds, J.H. and Newbury, H.J. (1988). Tissue culture conservation of fruit trees. FAO/International Board of Plant Genetic Resources Newsletter 73/74: 9-20

Zayova, E. Nevdev T. and Dimitrova, L. (2017). In vitro storage of Stevia rebaudiana Bertoni under slow growth conditions and mass multiplication after storage. Bio Bulletin, 3(1):30-38.

Published
2023-03-14
How to Cite
AbdulmalikM. M., & UsmanI. S. (2023). SLOW GROWTH IN VITRO CONSERVATION OF GROUNDNUT (Arachis hypogaea L.). FUDMA JOURNAL OF SCIENCES, 2(4), 55 - 60. Retrieved from https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/1318