ANTIOXIDANT ACTIVITIES OF ETHANOL LEAF EXTRACTS OF PSIDIUM GUAJAVA L., TERMINALIA CATTAPA L. AND THEIR COMBINED FORMULATION
DOI:
https://doi.org/10.33003/fjs-2022-0603-986Keywords:
Antioxidant, in vivo, Psidium guajava, Terminalia cattapa, Formulation, Leaf extractsAbstract
Free radicals in the body are the cause of many chronic and debilitating conditions in humans by acting as oxidants in the body. This research was aimed at assessing the antioxidant potential of the leaf extracts of Psidium guajava, Terminalia catappa and a combination of both. The leaves of both plants were independently extracted with ethanol. Equal portions of the extracts of both species were also combined to obtain a formulation of ratio 1:1. The extracts were subjected to in vitro assays including DPPH (2, 2-Diphenyl-1-picrylhydrazyl) and nitric oxide scavenging activities, while in vivo antioxidant assays were evaluated by measuring superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GPx) levels in Wistar rats following oral administration of 200, 400 and 800 mg/kg b.wt. respectively of the 1:1 extract formulation. The three extracts demonstrated dose dependent DPPH scavenging activity, although the 1:1 extract formulation was shown to possess lower free radical scavenging activity compared to the individual plant extracts. At the highest dose, the 1:1 extract formulation was shown to have exhibited a higher nitric oxide scavenging activity than the control, gallic acid. The 200 mg/kg b.wt. of 1:1 extracts formulation possess the highest antioxidant activity in the SOD and MDA assays. The study supports that ethanol leaf extracts of P. guajava and T. catappa possess potent antioxidant properties. However, additional work is required to obtain a more effective formulation from a combination of both extracts.
References
Abdullahi, A. A., Salaudeen, M. T Wada A. C. and Ibrahim, H. (2019). Germination and Longevity of Some Cowpea Cultivars Affected by Single and Mixed Virus Infections in Niger State, Nigeria. International Journal of Plant & Soil Science, 28 (5): 1-10. https://doi.org/10.9734/ijpss/2019/v28i530doi
Abdullahi, A. A., M. T. Salaudeen, M. G. M. Kolo and H. Ibrahim (2016) Serotypes and Prevalence of Legume Viruses in Niger State, Southern Guinea Savanna of Nigerian. Nigerian Journal for Plant Protection. 30 (1) 37-46
Adesina, G. O., Ajayi, S. A and Olabode, O. S. (2012). Influence of weed control methods on viability and vigour of maize (Zea mays L.) seeds. Nigerian Journal of Weed Science 25: 117-124
Ahmad, Z., Ghafoor, A. and Bashir, M. (2006). Effect of seed-borne pathogens on seed longevity in chickpea and cowpea under storage at 25 oC to 18 0C. Seed Science Technology. 34, 69-75
Agrios, G. N. (2005). Plant Pathology, Fifth Edition. Elsevier Academic Publishers. Amsterdam.
Alegbejo, M. D (2015). Virus and virus-like diseases of crops in Nigeria. Zaria, Nigeria Ahmadu Bello University Press. 273pp
Anjorin, S. T and Mohammed, M. (2014). Effects of seed-borne fungi on germination and seedling vigour of watermelon (Citrullus lanata Thumb). African Journal of Plant Science. 8(5). 232-236.
Bashir, M., Ahmad, Z and Murata, N. (2000). Seed-borne Viruses: Detection, Identification andControl. Islamabad, Pakistan,Pakistan Agricultural Research Council, National Agricultural Research Center, pp:156.
Batiano A. (2011). Fighting poverty in sub-Saharan Africa: The multiple roles of legumes in integrated soil fertility management. New York, Dordrecht.
Dugje, I. Y., Omoigui, L. O., Ekeleme, F., Kamara, A. Y andAjeigbe, H.(2009).Farmers’ Guide to Cowpea Production in West Africa. Ibadan, Nigeria, IITA, Pp 5-12
El Balla, M. M. A., Saidahmed, A. I and Makkawi, M. (2011). Effects of moisture contents and maturity on hardseededness and germination in okra (Albemoschus esculentus [L] Moench.) seeds. Weed science 35, 45-51
FAO (Food and Agriculture Organization) (2012). Available from http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567 #ancor
Golnaraghi, A. R., Shahraeen, N., Pourrahim, R., Farzadfar, S. H. and Ghasemi, A. (2004). Occurrence and relative incidence of viruses infecting soybeans in Iran. Plant Dis., 88:1069-1074.
Gómez, C. (2012). Cowpea: Post-harvest management; Rome, Italy. Food and Agriculture Organization (FAO), 71p
Hamim, I., Mohanto, D. C., Sarker, M. A and Ali, M. A. (2014). Effect of seed-borne pathogen on germination of some vegetable seeds. Journal of Phytopathology and Pest Management. 1,(1). 34-51.
IITA (International Institute for Tropical Agriculture). (2013) Research highlights, Ibadan, Nigeria IITA pp 143-146
Kareem, K. T andTaiwo, M. A (2007). Interactions of viruses in cowpea: Effects on growth and yield parameters. Virology Journal, 4, (1), 234-240.
Kumar, L. (2009). Methods for the diagnosis of Plants Virus diseases. Laboratory Manual, Ibadan IITA, 94pp
Mandhare, V. K and Gawade, S. B (2010). Effect of seed-borne Soybean mosaic virus infection on quality and yield parameters in soybean. Legume Research, 33,(1),43-49.
Matthews, R. E. F. (1991). Relationship between plant viruses and invertebrates. pp. 520-561. In: "Plant Virology". 520-561. 3rd Edition. Academic Press Inc. 835 pgs.
Nsa, I.Y, and Kareem, K.T. (2015). Additive interactions of unrelated viruses in mixed infections of Cowpea (Vigna unguiculata L.Walp). Frontier Plant Science, 6, 8:12
Omoigui, L.O. , A.Y. Kamara, F.S. Massawe, M.F Ishiyaku, O. Boukar , S.O. Alabi, and F. Ekeleme (2007). Evaluation of Cowpea Genotypes for their Reactions to Striga gesnerioides in the Dry Savanna of Northeast Nigeria. African Crop Science Conference Proceedings Vol. 8. pp. 273-278
Owolabi, A. T., Taiwo, M. A., and Mabadeje, S. A. (1988). Effects of single and mixed inoculations with Blackeye cowpea mosaic virus on two Nigerian cowpea cultivars. Niger. J. Basic Appl. Sci. 2, 25–33.
SAS [Statistical Analysis System] (2008). Statistical Analysis System SAS/STAT User’s guide, version. 9.2. Cary, N.C SAS Institute Inc.
Singh, G. (2014). Response of soybean (Glycine max) genotypes to plant population and planting geometry in Northern India. International Journal of Agricultural Resources, 6: 653-659.
Susana, F. T and Elena, S. P (2009). The inheritance and assessment of a second quantitative gene for blackeye cowpea mosaic virus in southern pea, (Vigna unguiculata [L.] Waip.) Auburn Univ. Auburn, Alabama, U.S.A.
Taiwo, M. A., and Akinjogunla O. J. (2006). “Cowpea viruses: Quantitative and qualitative effects of single and mixed viral infectionsâ€. African Journal of Biotechnology, 5 (19), 1749-1756.
Thottappilly, G., and Rossel, H. W. (1992). Virus of cowpea in tropical Africa. Trop. Pest Manage. 38, 337–348. doi: 10.1080/09670879209 371724
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