ISOLATION, CHARACTERIZATION AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF DIARRHEAGENIC ESCHERICHIA COLI (DEC) AMONG CHILDREN ATTENDING SOME SELECTED HOSPITALS WITHIN KADUNA METROPOLIS
Abstract
Diarrhoea has been associated with significant morbidity and mortality, ranked the second cause of death in children aged 0 to 5 years. Bacteria, viruses, protozoa, and helminths have all been implicated in diarrhoea, however rotavirus and diarrhoeagenic E. coli (DEC) are the most common cause. This study aimed to determine the antibiotic susceptibility pattern of diarrheagenic Escherichia coli isolated from children 0-5 years attending selected hospitals in Kaduna metropolis. A total of 264 stool samples were collected from children attending four selected hospitals in Kaduna metropolis. Standard culture procedures and molecular techniques such as PCR and 16s rRNA were employed in isolation and characterization of diarrhoeagenic E. coli from the stool samples. The study established a prevalence of 24.2% for diarrheagenic Escherichia coli, and all the isolates demonstrated multiple antimicrobial resistance index (MARI) of 0.5 and above, and showed significant resistance against augmentin (100%), amoxacillin (100%), ampicillin-cloxacillin (ampiclox) (100%), erythromycin (100%), gentamycin (100%), cefoxitin (100%), cefuroxime (95%), ceftriazone (95%) and ceftaxidime (85%). The least (60%) resistance was observed against imipenem. The study concluded that antibiotics have not been very effective in the treatment of E. coli-related diarrhoea. The study, therefore, recommends the implementation of programmes geared towards good hygiene, good nutrition and good health
References
Adesoji, A. T., & Liadi, A. M. (2020). Antibiogram studies of Escherichia coli and Salmonella species isolated from diarrheal patients attending Malam Mande General Hospital dutsin-ma, Katsina state, Nigeria. Pan African Medical Journal, 37.
Afunwa, R.A., Ezeanyinka, J., Afunwa, E.C., Udeh, A.S., Oli, A.N. and Unachukwu, M. (2020). Multiple Antibiotic Resistant Index of Gram-Negative Bacteria from Bird Droppings in Two Commercial Poultries in Enugu, Nigeria. Open Journal of Medical Microbiology, 10(4).
Agbla, J. M. M., Capo-Chichi, A., Agbankpé, A. J., Dougnon, T. V., Yadouleton, A. W. M., Houngbégnon, O., Glele-Kakai, C., Armah, G. E., and Bankolé, H. (2018). Epidemiological Survey of Rotaviruses Responsible for Infantile Diarrhea by the Immunomolecular Technique in Cotonou (Benin, West Africa). International Journal of Microbiology.
Akingbade, O.A., Akinjinmi, A.A., Olasunkanmi, O.I., Okerentugba, P.O., Onajobi, B.I. and Okonko, I.O. (2013). Bacterial organisms isolated from children with diarrhoea in Abeokuta, Nigeria. Stem Cell Journal, 4(4), 5- 9.
Alizade, H. (2018). Escherichia coli in Iran: An Overview of Antibiotic Resistance: A Review Article. Iranian Journal of Public Health, 47(1), 1–12.
Ayandele, A. A., Ajala, O. O., Oyekemi, S. A., and Awotunde, M. O. (2019). Microbiological evaluation and antimicrobial resistant pattern of bacteria isolated from surface drinking water sources in Ogbomoso, Oyo State, Nigeria. Nigerian Journal of Biotechnology, 36(1), 17.
Beshiru A, Okoh AI, Igbinosa, E.O. (2022) Processed ready-to-eat (RTE) foods sold in Yenagoa Nigeria were colonized by diarrheagenic Escherichia coli which constitute a probable hazard to human health. PLoS ONE 17(4): e0266059. https://doi.org/10.1371/journal.pone.0266059
Bruzzese, E., Giannattasio, A., & Guarino, A. (2018). Antibiotic treatment of acute gastroenteritis in children. F1000Research, 7: 193. https://doi.org/10.12688/f1000research.12328.1
Castro, A. M., Santos-Balbuena, H. F., GarcÃa-GarcÃa, A. E., & Arzate-Barbosa, P. (2019). Association of diarrheagenic Escherichia coli with virotypes and sensitivity to antimicrobials in children of the Mexico City. Revista Medica del Hospital General de Mexico, 82(2).
Cheesbrough, M. (2009). District laboratory practice in tropical countries, Part 2. Cambridge University Press.
Chioma, R. O., Mzungu, I., and Orpin, J. B. (2019). Antibiogram of Diarrhoeagenic Escherichia coli Patients under Five Years Attending Selected Hospitals in Kaduna State, Nigeria. Journal of Advances in Microbiology, 14(4), 1–11.
Clinical and Laboratory Standards Institute. (2012). Performance standards for antimicrobial disk susceptibility tests: Approved standard - Eleventh edition, 32 (1).
David, E. E., Yameen, M. A., Igwenyi, I. O., Okafor, A. C., Obeten, U. N., Obasi, D. O., … David, C. N. (2020). The frequency of virulent genes and antimicrobial resistance patterns of Diarrhoeagenic Escherichia coli isolated from stools of children presenting with diarrhoea in a tertiary hospital in Abakaliki, Nigeria. 6(2), 147–152. https://doi.org/10.14202/IJOH.2020.147-152
David, E., Yameen, M. A., Igwenyi, I. O., Iroha, I. R., Nzelibe, H. C., Shuaibu, M. N., ... and Onwe, O. S. (2019). The frequency of fluoroquinolone resistant enterotoxigenic Escherichia coli from stools of diarrheic children in Ebonyi State, Nigeria.
Eseigbe, E.E., Iriah, S., Ayuba, G.I., Ibok, S., Adama, S.J. and Eseigbe, P. (2013). Bacterial isolates from the stools of children aged less than 5 years with acute diarrhoea in Kaduna, Northwestern Nigeria. Annals of Tropical Medicine and Public Health, 6(4), 452-455.
Fair, R. J., and Tor, Y. (2014). Antibiotics and bacterial resistance in the 21st century. Perspectives in Medicinal Chemistry, 6, 25–64.
Franzolin, Marcia Regina et al. (2005). Prevalence of Diarrhoeagenic Escherichia coli in children with diarrhea in Salvador, Bahia, and Brazil.Memórias do Instituto Oswaldo Cruz [online]. 2005, v. 100, n. 4 [Accessed 3 November 2021] 359-363.
Gillespie, S. H., and Hawkey, P. M. (2006). Principles and Practice of Clinical Bacteriology: Second Edition. Principles and Practice of Clinical Bacteriology
Ifeanyi, C. C., Isu, N. I. R., Akpa, A. C., and Ikeneche, N. (2010). Enteric Bacteria Pathogens Associated With Diarrhoea of Children in the Federal Capital Territory Abuja , Nigeria. New York Science Journal, 3(1), 62–69.
Iseghohi, F., Galadima, M. and Kuta, F.A. (2021). Escherichia coli Encoding Extended-Spectrum Beta-Lactamases Isolated from Diarrheic Patients in Minna, Nigeria. The International Journal of Biotechnology, 10(2), 52 - 68.
Joseph, A.A. Odimayo, M.S. and Olokoba, L.B. Olokoba, A.B. & Popoola, G.O. (2017). Multiple Antibiotic Resistance Index of Escherichiacoli Isolates in a Tertiary Hospital in South-West Nigeria. Medical Journal of Zambia, 44(4), 225-232.
Kehinde, A., and Umar, U. (2018). Combating diarrhoea in Nigeria: the way forward. Journal of Microbiology & Experimentation, 6(4). https://doi.org/10.15406/jmen.2018.06.00213
Loha, A., Rahman, M. M., Biswas, A., Mou, M. H., Rifat, T. A., Choudhury, M. M., Jebin, F. M., Alarm, F. M., Sarker, P. K., Tariquzzaman, M., Husain, M. A., Noman, M. A., and Haroon, R. M. (2021). Antibiotic resistance studies of Escherichia coli and Salmonella species isolated from diarrheal patients. African Journal of Biological Sciences, 3(3), 122. https://doi.org/10.33472/afjbs.3.3.2021.122-130
Mandomando, I., Jaintilal, D., Pons, M. J., Vallès, X., Espasa, M., Mensa, L., Sigaúque, B., Sanz, S., Sacarlal, J., Macete, E., Abacassamo, F., Alonso, P. L., & Ruiz, J. (2009). Antimicrobial susceptibility and mechanisms of resistance in Shigella and Salmonella isolates from children under five years of age with diarrhea in rural Mozambique. Antimicrobial Agents and Chemotherapy, 53(6), 2450–2454.
Nikaido H. (2009). Multidrug resistance in bacteria. Annual review of biochemistry, 78, 119-146.
Olayinka, B., Olonitola, O., Olayinka, A., & Agada, E. (2004). Antibiotic Susceptibility Pattern and Multiple Antibiotic Resistance Index of Pseudomonasaeruginosa Urine Isolates From a University Teaching Hospital. African Journal of Clinical and Experimental Microbiology, 5(2), 198–202.
Rawat, D., & Nair, D. (2010). Extended-spectrum β-lactamases in Gram Negative Bacteria. Journal of global infectious diseases, 2(3), 263–274. https://doi.org/10.4103/0974-777X.68531
Rodrigo, M. G.-M. E.-L. (2020). Antimicrobial Resistance in Escherichia coli (p. Ch. 9). IntechOpen. https://doi.org/10.5772/intechopen.93115
Saka, H. K., Dabo, N. T., Muhammad, B., GarcÃa-Soto, S., Ugarte-Ruiz, M., & Alvarez, J. (2019). Diarrheagenic Escherichia coli Pathotypes from Children Younger Than 5 Years in Kano State, Nigeria. Frontiers in public health, 7, 348. https://doi.org/10.3389/fpubh.2019.00348
Sang, W. K., Oundo, V., & Schnabe, l.D. (2012). Prevalence and antibiotic resistance of bacterial pathogens isolated from childhood diarrhoea in four provinces of Kenya. The Journal of Infection in Developing Countries, 6(07): 572-578. https://doi.org/10.3855/jidc.2196
Spano, L. C., da Cunha, K. F., Monfardini, M. V., de Cássia Bergamaschi Fonseca, R., and Scaletsky, I. C. A. (2017). High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil. BMC Infectious Diseases, 17(1), 1–10.
Sulaiman, M. A., Muhammad , H., Muhammad Sani, A., Ibrahim, A., Hussain, I. M., and Anchau, Z. G. (2020). Evaluation of the impact of plasmid curing on antibiotic resistance on some clinical isolates of escherichia coli. Fudma journal of sciences, 4(3), 323 - 327.
Thenmozhi, S., Moorthy, K., Sureshkumar, B. T., and Suresh, M. (2014). Antibiotic Resistance Mechanism of ESBL Producing Enterobacteriaceae in Clinical Field: A Review. International Journal of Pure and Applied Bioscience, 2(3), 207–226.
Ugwu, M. C., Edeani, G. I., Ejikeugwu, C. P., Okezie, U., & Ejiofor, S. O. (2017). Antibiotic susceptibility profile of Escherichia coli and Salmonella causing childhood diarrhoea in Awka Municipality, South-Eastern Nigeria. Clin Microbiol, 6(277), 2.
Wilunda, C., & Panza, A. (2009). Factors associated with diarrhea among children less than 5 years old in Thailand: a secondary analysis of Thailand multiple indicator cluster survey 2006. Journal of Health Research, 23: 17-22.
World Health Organization. (2017, May 7). ''Diarrhoeal disease'' Retrieved from https://www.who.int/news-room/fact-sheets/detail/diarrhoeal-disease
Zeighami, H., Haghi, F., Hajiahmadi, F., Kashefiyeh, M., and Memariani, M. (2015). Multi-drug-resistant enterotoxigenic and enterohemorrhagic Escherichia coli isolated from children with diarrhea. Journal of Chemotherapy, 27(3), 152–155.
Zhang, S. X., Zhou, Y. M., Tian, L. G., Chen, J. X., Tinoco-Torres, R., Serrano, E., Li, S. Z., Chen, S. H., Ai, L., Chen, J. H., Xia, S., Lu, Y., Lv, S., Teng, X. J., Xu, W., Gu, W. P., Gong, S. T., Zhou, X. N., Geng, L. L., and Hu, W. (2018). Antibiotic resistance and molecular characterization of diarrheagenic Escherichia coli and non-typhoidal Salmonella strains isolated from infections in Southwest China. Infectious Diseases of Poverty, 7(1), 1–11.
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