EVALUATION OF IMMUNOLOGICAL MARKERS IN PATIENTS WITH PULMONARY TUBERCULOSIS IN SOME HOSPITALS IN UYO, AKWA IBOM STATE, NIGERIA
Abstract
Pulmonary tuberculosis (PTB) remains a major public health problem especially in the developing countries. Literature evidence opines that serological markers associated with PTB infections enhance early diagnosis. This study employed CD4+ T-lymphocyte counts and total serum immunoglobulin to establish early diagnosis in PTB patients in Uyo. Sputum samples of 105 patients were screened for Acid Fast Bacilli, while blood (7ml) was collected for CD4+ T-lymphocyte counts and quantification of total serum using cytoflow counter and Enzyme linked Immunoassay, respectively. A prevalence of 12.4% was established for PTB infection among the patients. The mean differences between immunoglobulins levels in relation to CD4+ T-lymphocyte counts was not significant at p ˃ 0.05. The mean IgG value of PTB patients was 737.29 ± 435.8 mg/dL, while the apparently healthy subjects (AHS) had the mean IgG value of 23.15 ± 32.2 mg/dL. The mean IgA level in PTB patients (190.91 ± 94.8 mg/dL) was higher than that of AHS (126.81 ± 35.5 mg/dL). The mean IgM value of PTB patients and that of the AHS was 317.75 ± 146.11 and 50.00 ± 32.3 mg/dL, respectively. There was significant difference between the immunoglobulin levels of PTB patients and AHS at p > 0.05 with respect to IgA and at p > 0.001 with respect to IgG and IgM. This study has indicated that biomarkers of humoral immunity were significantly affected when compared with AHS, thus, this study would enhance knowledge of the health practitioners and enable them to grasp the role of biomarkers in
References
Abdul KA, Andrew HL. (2009). Basic Immunology: Functions and Disorders of the Immune System. Saunders Elsevier. pp 212-222.
Akinjogunla, O. J., Ekuma, E.A., Etukudo I. U., Oshosanya, G.O. and Inyang, D. E. (2020). CD4+ T- lymphocyte values, bloodstream bacterial isolates and their antibiotic susceptibility profiles among human immunodeficiency virus infected patients in Uyo, Nigeria. Tropical Journal of Natural Product Research, 4 (9): 612-620.
Amilo GI, Meludu SC, Onyenekwe C, Ekejindu OC, Ifeanyichukwu O. (2012). Evaluation of CD4+ and CD8+ T- lymphocyte counts and serum levels of immunoglobulins in pulmonary tuberculosis (PTB) patients with or without HIV coinfection in South Eastern Nigeria. African Journal of Pharmacy and Pharmacology. 6(23):1639-1643.
Arinola OG, Igbi J. (1998). Serum immunoglobulin and circulating immune complexes in Nigeria with human immunodeficiency virus and pulmonary tuberculosis infection. Tropical Journal of Medical Research. 2(2):41-48.
Cheesbrough M. (2006). District Laboratory Practice in Tropical countries. Low Price editions, UK: Cambridge University Press, Pp, 39-78, 209.
Federal Ministry of Health - Nigeria. (2012) Report first national TB prevalence survey 2012, Nigeria. Abuja: Federal Ministry of Health.
Gomez MP, Donkor S, Adetifa IM, Ota MOC, Sutherland JS. (2012). Analysis of LAM and 38 kDa Antibody levels for diagnosis of TB in a case-control study in West Africa. International Scholarly Research Network ISRN Immunology, 23:1-6.
Goodridge A, Zhang T, Miyata T, Sangwei LU, Riley LW. (2014). Antiphospholipid IgM antibody response in acute and chronic Mycobacterium tuberculosis mouse infection model. Clin Respir J., 8: 137-144.
Itah AY, Udofia SM. (2005). Epidemiology and Endemically of Pulmonary Tuberculosis (PTB) in South-Eastern Nigeria. South Eastern Asian Tropical Medicine Public Health, 36(2): 317-323.
Jain VK, Bishnoi HS, Beniwal OP, Misra S N. (1984). Immunoglobulin profile in pulmonary tuberculosis. Journal of Postgraduate Medicine, 30(80): 1-5.
Kent PT, Kubica GP. (1985). Public Health Mycobacteriology: A Guide for Level III Laboratory. Atlanta: Center for Disease Control. pp 10-154.
Kochak HE, Seyed-Alinaghi S, Zarghom O, Hekmat S, Jam S, Abdi Z. (2010). Evaluation of serological tests using A60 antigen for diagnosis of tuberculosis. Acta Med Iran, 48: 21-26.
Lawn SD, Acheampong JW. (2009).Pulmonary tuberculosis in adults: factors associated with mortality at a Ghanaian teaching hospital. West Afr J Med. 18(4):270-274.
Lyamuya EF, Maselle SY, Matre R. (1999). Serum Immunoglobulin Profile in Asymptomatic HIV-1 Seropositive Adults and in Patients with AIDS in Dares Salaam Tanzania. East African Medical Journal. 76 (7): 370-375.
Nwachukwu E, Peter GA. (2010). Prevalence of Mycobacterium tuberculosis and human immuno-deficiency virus (HIV) infections in Umuahia, Abia state, Nigeria. African Journal of Microbiology Research, 4 (14):1486-1490.
Nzou C, Kambarami RA, Onyango FE, Ndhlovu CE, Chikwasha V. (2010). Clinical predictors of low CD4 count among HIV-infected pulmonary tuberculosis clients: A health facility- based survey. S. Afri Med J. 100:602–605.
Olaniyi JA, Arinola GO. (2011).Humoral immunological factoral and nitrioxide levels in HIV Patients with low CD4+ T-lymphocytes counts. International Journal of Health Research. 4(2): 69-74.
Schneider BE, Korbel D, Hagens K, Koch M, Raupach B, Enders J. (2010). A role for IL-18 in protective immunity against Mycobacterium tuberculosis. Eur. J. Immunol. 40: 396–405.
Selma WB, Harizi H, Boukadida J. (2011).Immunochromatographic IgG/IgM test for rapid diagnosis of active tuberculosis, Clin Vaccine Immunol., 18(12): 2090–2094.
Tripathy S, Meron P, Joshi DR, Patil U, Gadkari DA, Paranjape RS. (2000). CD4 and CD8 Lymphocytes counts in Mycobacterium tuberculosis in pure India. Indian Journal of Medical Research, 111: 195-198.
Umeh EU, Ishaleku D, Iheukwumere CC. (2007). HIV/Tuberculosis co-infection referred chest clinic in Nasarawa State, Nigeria. Journal of Applied Science, 7: 1-9.
Umo AN, Akinjogunla OJ, Umoh NO, Uzono GE. (2020). Diagnosis and risk factors of latent tuberculosis infection among healthcare workers using whole blood human interferon-gamma release assay and tuberculin skin testing. Asian Journal of Research in Infectious Diseases, 3 (3):15-21.
Verma S, Mahajan V. (2008). HIV- Tuberculosis Co-infection. The Internet Journal of Pulmonary Medicine. 10(1): 1531-2984.
Wilson D, Badri M, Maartens G. (2011). Performance of serum C-reactive protein as a screening test for smear-negative tuberculosis in an ambulatory high HIV prevalence population. PLoS One. 6(1):e15248.
World Health Organization (2017). Global tuberculosis report 2017. Geneva: World Health Organization.
World Health Organization (WHO) (2009). A guide to monitoring and evaluation for collaborative TB/HIV activities, World Health Organization.
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