SOMATIC EMBRYOGENESIS IN LOCAL COWPEA (Vigna unguiculata L. (WALP) VARIETIES
DOI:
https://doi.org/10.33003/fjs-2022-0601-895Keywords:
Organogenesis, Explant, Somatic EmbryogenesisAbstract
Organogenesis in vitro was evaluated in explants derived from shoot apices of two local cowpea varieties cultured on Murashige and Skoog (MS) basal medium. To evaluate the response of the cowpea genotypes to in vitro regeneration, seedlings were drived from zygotic embryos excised from surface sterilized seeds after two weeks of culture on MS basal medium + vitamins supplement with 30% sucrose. To regenerate shoots, apices of the In Vitro seedlings with average length of 1cm were excised and cultured on MS supplemented with 0.5, 1.0 or 1.5mg/L 6-Benzylamino purine (BAP) with or without 0.1mg/L α-naphthaleneacetic acid (NAA) for six weeks. Variation was observed in response of the shoot apices from different cowpea cultivars to in vitro regeneration of shoots. The highest shoot number was recorded with Danilla and shoot length was highest in Kanannado, indicating that Danilla responded well to in vitro regeneration. While there were significant differences between these concentrations and other concentrations used in this study on the number of shoots produced, statistically there was no difference between the traetments tested on the in vitro shoot length recorded in this study.Although shoots were succesfully regenerated in this study rooting was not achieved as the explants gradually transformed into calli
References
Hipel, K.W. & McLeod, A.I. (1994). “Time Series Modelling of Water Resources and Environmental Systemsâ€, Amsterdam, Elsevier.
Iqbal, M. (1983). An Introduction to Solar Radiation. Academic, Toronto.
Jadevicius, A. & Huston, S. (2015). ARIMA modelling of Lithuanian house price index, International Journal of Housing Markets and Analysis, 8 (1), 135-147.
John, A.D. & William, A.B. (2013). Solar Engineering of Thermal Processes Fourth Edition. John Wiley & Sons, Inc. Published.
Lincoln, Z., Michael, D.D., Consuelo, C.R., Kati, W.M. and Kelly, T.M. (2015). Step by Step Calculation of the Penman-Monteith Evapotranspiration (FAO-56 Method). Journal of Agricultural and Biological Engineering Department, UF/IFAS Extension.
Mst. Islam, T. & Zakaria, Md. (2019). Forecasting of Maximum and Minimum Temperature in the Cox’s Bazar Region of Bangladesh based on Time Series Analysis. IOSR Journal of Mathematics (IOSR-JM) Volume 15, Issue 5, PP 56-67.
Musa, J. J. (2013). Stochastic Modelling of Shiroro River Stream flow Process. American Journal of Engineering Research (AJER), Volume-02, Issue-06, pp-49-54
Mohammed, H. A., Mohammad, K. Y. & Jeong K. (2019). Time Series ARIMA Model for Prediction of Daily and Monthly Average Global Solar Radiation: The Case Study of Seoul, South Korea. Symmetry, 11, 240, pp 1-17.
Nashirah, A. & Sofian, R. (2017). Autoregressive Integrated Moving Average (ARIMA) Model for Forecasting Cryptocurrency Eychange Rate in High Volatility Environment: A New Insight of Bitcoin Transaction. International Journal of Advanced Engineering Research and Science (IJAERS), 4 (11) p130- 137
Nyatuame, M. & Agodzo, S.K. (2018). Stochastic ARIMA model for annual rainfall and mayimum temperature forecasting over Tordzie watershed in Ghana. Journal of Water and Land Development. 37 p. 127–140.
Santiago, A.V., Pereira, A.R., Folegatti, M.V. & Maggiotto, S.R. (2002). Reference Evapotranspiration Measured with a Weighing Lysimeter and Estimated by Penman-Monteith (FAO-56) on a Monthly and Ten-Days Time Scales. Revista Brasileira de Agrometeorologia, 10, 57-66.
Spencer, J.W. (1971). Fourier Series Representation of the Position of the Sun. Search, 2(5), pp172.
Tukey, J. W . (1977). EDA. Reading, 1 Mass.: Addison-Wesley, p. 43.
Von Randow, R.C.S. & Alvalá, R.C.S. (2006). Estimation of Long-Wave Atmospheric Radiation over Pantanal Sul Mato Grossense during the Dry Seasons of 1999 and 2000. Revista Brasileira de Meteorologia, 21, 398-412.
Published
How to Cite
Issue
Section
FUDMA Journal of Sciences
