PHYSIOLOGICAL RESPONSES AND TOLERANCE EFFICIENCY OF Spinacea oleracea L. UNDER HYDROPONIC Ni2+ STRESS CONDITION
Abstract
An indoor EDTA and HNO3 enriched environment was created by an injection system with timing and varying concentration control. Spinacea oleracea was selected hydroponic plant exposed to various doses of Ni2+ (0, 1000, 2000 and 4000 mg/L) as Na2EDTA at (0, 500 and 3000 mg/L) and (0,500 and 3000 mg/L) HNO3 in different combinations for 6 days with 10-hour-treatment each day. This study used modified Hoagland nutrient culture in a screen house to provide an ideal environment for comparing the efficiency of chelate-assisted and unchelated phytoextraction of Ni2+ by S. oleracea. Changes in morphological characteristics including leaf damage rate to evaluate morphological resistance to Ni2+ uptake and proline contents was observed. Changes in fresh biomass were significant (p < 0.05) with respect to addition of EDTA and HNO3 at different concentration to different concentrations of Ni2+ compared to unchelated treatments of same concentrations of Ni2+. The Ni2+ induced proline accumulation in shoots increased significantly (P < 0.05) with increasing Ni2+ concentrations.
References
Chen, C., Huang, D. and Liu, J. (2009). Functions and Toxicity of Nickel in Plants A Review: Recent Advances and Future Prospects. Clean 2009, 37 (4), 304 – 313.
Dan, T.V., Krishnaraj, S., Saxena and P.K (2002). Cadmium and nickel uptake and accumulation in scented Geranium (Pelargonielm sp.'Frensham'), Water Air Soil Pollut. 137(1): 355 – 364.
Durman, F. and Ozturk, F. (2009).Nickel accumulation and its effect on biomass, protein content and antioxidative enzymes in roots and leaves of water cress(Nasurium o_cinale ) Journal of Environmental Sciences, 22(4):526-532.
Eitinger, T., Mandrand-Berthelot, M.A. (2000). Nickel transport systems in microorganisms, Arch. Microbiol. 173(1): 1–9.
Giordani, C., Cecchis, S. and Zanchi, C. (2005). Phytoremediation of Soil Polluted by Nickel Using Agricultural Crops .Environmental Management. 36(5): 675-681.
Gajewska, E., Sklodowska, M., Slaba, M., and Mazur, J. (2006). Effect of nickel on antioxidative enzyme activities, proline and chlorophyll content in wheat shoots. Biological Plant. 50(4):653-659.
Hussain, M. B., Shafaqa, T.A., Aqeel, A., Saadia, H., Muhammad, A.F., Basharat, A., Saima, A.B and Muhammad, B. G.(2013). Morphological, physiological and biochemical responses of plants to nickel stress: A review. African Journal of Agricultural Reserve.8 (17)1596-1602.
Jean, L., Bordas, F., Gautier-Moussard, C., Vernay, P. (2008). Effect of citric acid and EDTA on chromium and nickel uptake and translocation by Datura innoxia. Environmental Pollution., 153(3):555-563.
Jean, L., Bordas, F., Gautier-Moussard, C., Vernay, P. (2008). Effect of citric acid and EDTA on chromium and nickel uptake and translocation by Datura innoxia. Environ. Pollut., 153(3):555-563.
Kiple, F. Kenneth (2002). Cambridge World History of Food. 2nd edition, United Kingdom: Cambridge, p 288
Kovacs E, Nyitrai P, Czovek P, Ovari M, Keresztes A (2009). Investigation into the mechanism of stimulation by low-concentration stressors in barley seedlings. Journal of Plant Physiolology 166(1):72-79.
Mamdouh, A.Eissa;Mohamed F.Ghoneim;Galal A. ElGharably and Mohamed Abd El- Razek.(2014). Phytoextraction of Nickel, Lead and Cadmium from Metals Contaminated Soils Using Different Field Crops and EDTA. World Applied Sciences Journal. 32(6):1045-1052.
Meers, E., Tack, F. M. G., Van Slycken, S., Ruttens, A., Vangronsveld, J., Verloo, M. G.(2008).Chemical- assisted phytoextraction: A review of potential soil amendments for increasing plant uptake of heavy metals. International .Journal of Phytoremediation. 10(5):390-414.
Pandey, S.N. (2006).Accumulation of heavy metals (Cd, Cr, Cu, Ni and Zn) in Raphanus sativus Land Spinacia oleracea L. plants irrigated with industrial effluent. Journal of Environmental Biology, 27(2):381-384.
Sengar, R.S., Shalini, G., Madhu, G., Amal, S., and Sengar, K. (2008).Occurrence, Uptake, Accumulation, and Physiological Responses of Nickel in Plants and Its Effect on Environment. Journal of phyto-Chemistry.2 (2):44-60.
Seregin, I.V., Kozhevnikova, A.D. (2006). Physiological role of nickel and its toxic effects on higher plants, Russ. Journal of Plant Physiology 53(2): 257 – 277.
Singh, K. and Pandey, S.N.(2011). Effect of nickel-stresses on uptake, pigments and antioxidative responses of water lettuce, Pistia stratiotes L. Journal of Environmental Biology. .32(3): 391-394.
Vajpayee, P., Rai, U.N., Ali, M.B., Tripathi, R.D., Yadav, V., Sinha, S. and Singh, S.N. (2001). Chromium – Induced changes in Vallisneria spiralis L.and its role in phytoremedation of tannery effluents. Bulletin Environment and .Toxicology, 67(2): 246-256.
Vogel-Mikus, K., Drobne, D. and Regvar, M. (2005. Zn, Cd and Pb accumulation and arbuscular mycorrhizal colonization of pennycress Thlaspi praecox Wulf. (Brassicaceae) from the vicinity of a lead mine and smelter in Slovenia, Environmental Pollution 133(2): 233 – 242.
Yoon, J., Ca, X., Zhou, Q. and Ma, LQ. (2006). Accumulation of Pb, Cu, and Zn in native plants growing on a contaminated Florida site. Science Total Environment, 368(2): 456–464.
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