METHANOL LEAF EXTRACT OF Jatropha tanjorensis Ellis and Saroja POSSESS PHYTOCONSTITUENTS WITH FREE RADICAL SCAVENGING ACTIVITY
The body during normal metabolic function produces free radicals which are highly reactive species. Free radicals could also be introduced into the body from the environment. The oxidation induced by reactive oxygen species can result in DNA mutation, membrane protein damage and cell membrane disintegration. The present study assayed the preliminary phytochemicals, total phenolics and total flavonoids and free radicals scavenging ability of methanol extract of Jatropha tanjorensis leaf. Standard methods for determining phytochemicals, reducing power, nitric oxide scavenging, hydroxyl radical, and lipid peroxidation scavenging activity were employed. The phytochemical screening result revealed the presences of phenols, flavonoids, saponin, alkaloids, tannins, terpernoids and steroids. The total phenolic content of methanol extract of Jatropha tanjorensis leaf measured by Folin-Ciocalteau reagent in terms of gallic acid equivalent was 11.35±0.82mgGAE/g. The flavonoid content of the plant sample calculated as Quercetin equivalent was 15.91±1.60mgQCE/g. GC-MS results revealed relevant pharmacological bioactive compounds. The antioxidative activity of the plant’s extract correlated with total phenolic content. The radical scavenging activity showed a dose dependent increase in the reducing power. The minimum NO inhibitory activity was 8.88±0.63 at 200µg/ml and the maximum activity was 32.70±2.71 at 800µg/ml. The minimum percentage H2O2 radical inhibitory activity was 8.30±0.88 at 200µg/ml and a maximum activity was 22.80±2.28 at 800µg/ml. There was also an increase scavenging effect of lipid peroxide radical in concentration dependent manner. The results of this study indicate that the leaf of Jatropha tanjorensis possess antioxidant properties and could serve as free radical inhibitor
Arika, W.M., Abdirahman, Y.A., Mawia, M.A., Wambua. K.F., Nyamai, D.W. (2015). In Vivo Antidiabetic Activity of the Aqueous Leaf Extract of Croton macrostachyus in Alloxan Induced Diabetic Mice. Pharmceutica Analytica Acta. 6: 447-457.
Asif, M. (2015). Chemistry and antioxidant activity of plants containing some phenolic compounds. Chemistry International, 1(1): 35-52.
Atanu, F.O., Avwioroko, O.J., Momoh, S. (2018). Anti-diabetic Effect Of Combined Treatment With Aloe Vera Gel And Metformin On Alloxan-Induced Diabetic Rats. Journal of Ayurvedic and Herbal Medicine, 4(1): 1-5.
Cassidy, A., Hanley, B., Lamuela-Raventos, R.M. (2000). Isoflavones, Lignans And Stilbenes-Origins, Metabolism And Potential Importance To Human Health. Journal of the Science of Food and Agriculture. 80:1044-1062.
Chigozie, O.O., Uzoma, N.O., Ikechukwu, U.R, Ikechukwu, E.S. (2018). Nutritional Composition of Jatropha tanjorensis Leaves and Effects of its Aqueous Extract on Carbon Tetrachloride Induced oxidative Stress in Male Wistar Albino Rats. Biomedical Research, 29(19): 3569-3576.
Chiu-Yin KWAN, A.F.I. (2002). Tetrandrine And Related Bis-Benzylisoquinoline Alkaloids From Medicinal Herbs: Cardiovascular Effects And Mechanisms Of Action. Acta Pharmacologica Sinica. 23: 1057-1068.
Danborno, A.M., Tarfa, F., Toryila1, J.E., Awheela, E.U. and Shekarau, V.T. (2019). The Effects of Jatropha tanjorensis Aqueous Leaf Extract on Haematological Parameters in Wistar Rats. J. Afr. Ass. Physiol. Sci. 7 (2): 133-137.
Dholi, S.K., Raparla, R., Mankala, S.K., Nagappan, K. (2011). Invivo Antidiabetic Evaluation Of Neem Leaf Extract In Alloxan Induced Rats. Journal of Applied Pharmaceutical Science, 1:100-105.
Gulcin I. (2006). Antioxidant and antiradical activities of L-carnitine. Life Sci. 78:803e811.
Holst, B. and Williamson, G. (2008). Nutrients and phytochemicals: from bioavailability to bioefficacy beyond antioxidants. Current opinion in biotechnology, 19:73-82.
Joshi, S.S., Kuszynski, C.A., Bagchi, D. (2001). The Cellular and Molecular Basis Of Health Benefits Of Grape Seed Proanthocyanidin Extract. Current Pharmaceutical Biotechnology, 2: 187-200.
Katan, M.B., Grundy, S.M., Jones, P., Law, M., Miettinen, T. (2003). Efficacy and Safety Of Plant Stanols And Sterols In The Management of Blood Cholesterol Levels. In Mayo Clinic Proceedings, 78: 965-978.
Khan, W., Subhan, S., Shams, D.F., Afridi, S.G., Ullah, R., 4 Abdelaaty A. Shahat , A.A., and Alqahtani, S.A. (2019). Antioxidant Potential, Phytochemicals Composition, and Metal Contents of Datura alba. Hindawi BioMed
Research International. Volume 2019, Article ID 2403718, 8 pages. https://doi.org/10.1155/2019/2403718.
Meir S, Kaneer J, Akiri B. (1995). Determination and involvement of aqueous reducing compounds in oxidative defense system of various senescing leaves. J Agric Food Chem. 43:1813e1815.
Meles, D.K., Adnyana, D.A., Rinaldhi, C.P., Octaviani, R.R., Cempaka, D.S. (2019).The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus). EurAsian Journal of BioSciences, 13(2): 757-762.
Muriithi, N.J., Maina, G.S., Maina, M.B., Kiambi, M.J., Juma, K.K., (2015). Determination of Hematological Effects of Methanolic Leaf Extract of Vernonia lasiopus in Normal Mice. Journal of Blood &Lymph. 5: 139-149.
Njoku, O.V., Chidi, O. (2009). Phytochemical constituents of some selected medicinal plants. African Journal of Pure and Applied Chemistry, 3(11): 228-233.
Nyamai, D.W., Arika, W., Ogola, P.E., Njagi, E.N.M., Ngugi, M.P. (2016). Medicinally Important Phytochemicals: An Untapped Research Avenue. J. Pharmacognosy and Phytochemistry Research and Reviews. 4:35-49.
O’Neill, F.H., Sanders, T.A., Thompson, G.R. (2005). Comparison Of Efficacy Of Plant Stanol Ester And Sterol Ester: Short-Term And Longerterm Studies. The American Journal of Cardiology, 96: 29-36.
Oladele, J., Oladele, O.T., Ademiluyi, A.O., Oyeleke, M.O., Awosanya, O.O., Oyewole, I.O. (2020) Chaya (Jatropha Tanjorensis) Leafs Protect Against Sodium Benzoate Mediated Renal Dysfunction And Hepatic Damage In Rats. Clinical Phytoscience, 6:13-23.
Olayiwola, G., Iwalewa, E.O., Omobuwajo, O.R., Adeniyi, A.A., Verspohi, E.J. (2004). The antidiabetic potential of Jatropha tanjorensis leaves. Nig J Nat Prod Med. 8:55-8.
Omoregie, E.S. and Osagie, A.U. (2007). Phytochemical Screening And Anti-Anemic Effect Of Jatropha tanjorensis Leaf In Protein Malnourished Rats. Plant Archives, 7:509-516.
Oyewole, O.I. and Akingbala, P.F. (2011). Phytochemical Analysis and Hypolipidemic Properties of Jatropha tanjorensis Leaf Extract. European Journal of Medicinal Plants, 1(4): 180-185.
Pallab, K., Tapan, B., Tapas, P. and Ramen, K. (2013). Estimation of total flavonoids content (TPC) and antioxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. Journal of Drug Delivery and
Therapeutics,3(4): 33-37. Pavithra, K., Vadivukkarasi, S. (2015) Evaluation Of Free Radical Scavenging Activity Of Various Extracts Of Leaves From Kedrostis Foetidissima (Jacq.) Cogn. Food Science and Human Wellness, 4(1): 42-46.
Rahman, M.M, Islam, M.B., Biswas, M., Alam, A.K. (2015) In Vitro Antioxidant And Free Radical Scavenging Activity Of Different Parts Of Tabebuia Pallida Growing In Bangladesh. BMC Research Notes, 8(1):621-6.
Ros, E. (2000). Intestinal Absorption Of Triglyceride And Cholesterol; Dietary And Pharmacological Inhibition To Reduce Cardiovascular Risk. Atherosclerosis, 151: 357-379.
Shen SC, Lee WR, Lin HY, Huang HC, Ch Ko, Yang LL. (2002). In vitro and in vivo inhibitory activities of rutin, wogonin, and quercetin on lipopolysaccharide-induced nitric oxide and prostaglandin E(2) production. Eur J Pharmacol. 446:187e194.
Vanhanen, H.T., Blomqvist, S., Ehnholm, C., Hyvönen, M., Jauhiainen, M., (1993). Serum Cholesterol, Cholesterol Precursors, And Plant Sterols In Hypercholesterolemic Subjects With Different Apoe Phenotypes During Dietary Sitostanol Ester Treatment. Journal of Lipid Research. 34:1535-1544.
Wagner, K.H., Elmadfa, I. (2003). Biological relevance of terpenoids; Overview focusing on mono-, di- and tetraterpenes. Ann Nutr Metab., 47(3):95-106.
Yang, C.S., Landau, J.M., Huang, M.T., Newmark, H.L. (2001). Inhibition Of Carcinogenesis By Dietary Polyphenolic Compounds. Annual Review of Nutrition, 21: 381-406.
Copyright (c) 2021 FUDMA JOURNAL OF SCIENCES
This work is licensed under a Creative Commons Attribution 4.0 International License.
FUDMA Journal of Sciences