BRINE SHRIMP LETHALITY ASSAY AND LARVICIDAL ACTIVITY OF N-HEPTADECYL-N-(8-OCTA-1, 3-DIENE-6-YNYL)AMINE (C25H44N) ISOLATED FROM COMBRETUM MOLLE R.

Authors

Keywords:

Brine Shrimp, Larvicidal, Combretum molle, Artemia salina, Culexquinquefasciatus Say

Abstract

The extracts of leaves of Combretum molle were tested against the larvae of Artemia salina, i.e. Brine Shrimp Lethality Test (BST) and Culexquinquefasciatus Say (filaria disease vector) i.e. Larvicidal Test, using crude ethanol, n-hexane, chloroform, ethyl acetate, and methanol extracts. The methanolic extract proved to be the most effective in inducing complete lethality at minimum doses both in the BST and the Larvicidal activity test. The LC50­ values obtained are 24.85 µg/ml and 0.4µg/ml respectively. The Bioactivity result was used to guide the column chromatography which afforded the pure compound ACM 2 which was not active in the BST with LC50 value of 1259 µg/ml but was active in the Larvicidal activity test with LC50 value 15.14 µg/ml.  ACM 2 was proposed to be N-heptadecyl-N-(8-octa-1, 3-diene-6-ynyl)amine (C25H44N).

Dimensions

Abubakar , M. S. and Abdurrahman, E. M. (1998). Useful Plants in Traditional Control of Insects Pests. J. Herbs, Spices and Medicinal Plants. 6 (2):49-54.

Adoum, O. A. (2006):- Control of Clavigraliatomentosicollis, Marucavitrata and Spodoteraexempta with plant extracts. Ife J. of Sci. 8 (1) 9– 13.

Adoum, O.A. (2004):- Hatchability Effect of C18- Diacetylenic Fatty Acid of Xiemeniaamericana L. on Clavigraliatomentosicollis Eggs. J/ Agr. Sci and Technology 14 (1 and 2) 93 – 97.

Alkofahi A., Batshoun R., Owais W., and Najib N., (1996), Biological activity of some Jordanian, medicinal plant extracts. Fitoterapia. 68(5): 435.

Anderson J.E., Chang C.J. and McLaughlin J.L., (1988), Bioactive components of Allamanda Schottii. J. Nat. Prod., 51: 307.

Arias, J. and Mulla, M. S. (1975): Post emergence of Two Insects Growth Regulators on the Mosquito Culextarsalis (Diphtheria Culicidae). Journal of Medicinal Research. 12, 317-322.

Darsie,Jnr, R.F. and Ward R. A. (1960) Identification and Geographical Distribution of the Mosquitoes of North America, North of Mexico, American Mosquito Control Association,vol 1 pp 456-462.

Itharat, A., Houghton, P.J., Eno-Amooquaye, E., Burke, P.J., Sampson, J.H., and Ramam, A. 2004. In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer. J. Ethnopharm. 90:33-38.

Itharat, A., Supavita, T., Jusanit, P., Singchangchai, P., Subchareon, P., Deevisad, G., Ubonkaw, P., Ratanasuwan, P., Kajpunyapong, V., and Muangsrinun, R. 1999. Survey of medicinal plants and local folk wisdom in Southern Thailand: case study at Talebun National Parks. Research Report, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, p82-115.

Ferrigni N.R., Putnam J.E., Anderson B., Jacobson L.B., Nicholas D.E., Moore J.L., Mclaughlin J.L., Powell R.G., and Smith C.R., (1982), Modification and evaluation of the potato disc assay and antitumor screening of euphorbiaceae seeds. J. Nat. Prod., 45: 679.

Keawpradub, N., Amooqyaye, E.E., Burke, PJ., and Houghton, PJ. 1999. Cytotoxic activity of indole alkaloids from Alstonia macrophylla. Planta Med. 65: 311-315.

Keawpradub, N., Houghton, P.J., Amooqyaye, E.E., and Burke, P.J. 1997. Activity of extracts and alkaloids of Thai Alstonia species against human lung cancer cell lines. Planta Med. 63: 97-101.

Laupattarakasem, P., Houghton, P.J., Hoult, J.R.S., and Itharat, A. 2003. An evaluation of the activity related to inflammation of four plants used in Thailand to treat arthritis. J. Ethnopharm., 85: 207-215.

Matthes, H.W.D., Luu, B., and Ourisson, G. 1980. Cytotoxic components of Zingiber zerumbet, Curcuma zedoaria and C. domestica. Phytochem. 19: 2643-2650.

McLaughlin J.L., (1991), Methods in plant biochemistry, K. Hostettman (ed.), London: Academic press, 6: 1.

Meyer B.N., Ferrigni N.R., Putnam J.E., Jacobsen L.B., and McLaughlin J.L., (1982), Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica, 54: 31.

Mukhtar, M. D. and Tukur A. (2000): In-vitro Screening for anti microbial activity of PistiastratiotesL.extracts. Journal of Experimental Biology, vol. 1 (1) : 59-69

Mukhtar, M. D., Sani A. and Yakasai A. A .(2004): Cytotoxicity of extracts of Pistiastratiotes L. on Culex mosquito and A. salina larvae. Animal Research International. Vol. 1 (2), 95-99.

Skehan, P., Storeng, R., Scudiero, D., Monks, A., McMahon, I., Vistica, D., Waren, JT., Bokesch, H., Kenney, S., and Boyd, M.R. 1990. New colorimetric cytotoxicity assay for anticancer drug screening. J Natl. Cancer Inst. 82:1107-1112.

Smithinan, T. 2001. Thai plant names. The Forest Herbarium Royal Forest Department, Bangkok . Subchareon, P. 1998. Handbook of anticancer : Thai traditional medicine : new concept for treated cancer. Thai traditional medicine Institute, Bangkok, p-3.

Sofowora, E.A. (1982):- Medicinal plants and traditional medicine in Africa. John Wiley and Sons, New York pp 71 – 221.

Suffness, M., and Pezzuto, J.M. 1990. Assays Related to Cancer Drug Discovery. In K Hostettmann (ed), Methods in Plant Biochemistry. Vol. 6. Assays for Bioactivity. Academic Press, London, pp.71-133.

Syu, W.J., Shen, C.C., Don, M.J., Ou, J.C., Lee, G.H. and Sun, C.M. 1998. Cytotoxicity of curcuminoids and some novel compounds from Curcuma zedoaria. J. Nat. Prod. 61:1531-1534

Taura D.W. Mukhtar M. D. and Adoum O.A. (2004): Lethality of the Aqueous Extracts of A. nilotica, G. senegalensis, K. Africana and S. longepedunculata on Culex mosquito larvae. Ife Journal of Science 6 (2).45-49

Vibha P., Veena A., Raghavendra K. and Dash A. P. (2007) Strong Larvicidal activity of three species of Spilanthes (Akarkara) against malaria (Anopheles Stephensi Liston, Anopheles Culicifacies, species C) and filarial vector (Culexquinquefasciatus Say). Parasitol Res. (102) 171-174.

William, K. (1975):- Organic Spectroscopy, Macmillan Education Ltd. Hampshire pp 19 – 167.

Wu, T.S., Tien, H.J., Yeh, M.Y., and Lee, K.H. 1988. Isolation and cytotoxicity of rhinacanthin-A and B, two naphthoquinone from Rhinacanthus nasutus. Phytochem. 27: 3787-3788

Wu, T.S., Hsu, H.C., Wu, P.L., Teng, C.M. and Wu, Y.C. 1998. Rhinacanthin-Q, A naphthoquinone from Rhinacanthus nasutus and its biological activity. Phytochem. 49: 2001-2003.

Published

10-07-2021

How to Cite

BRINE SHRIMP LETHALITY ASSAY AND LARVICIDAL ACTIVITY OF N-HEPTADECYL-N-(8-OCTA-1, 3-DIENE-6-YNYL)AMINE (C25H44N) ISOLATED FROM COMBRETUM MOLLE R. (2021). FUDMA JOURNAL OF SCIENCES, 5(2), 166-172. https://doi.org/10.33003/fjs-2021-0502-636

Issue

Vol. 5 No. 2 (2021): FUDMA Journal of Sciences - Vol. 5 No. 2

Section

Research Articles
Copyright & Licensing

FUDMA Journal of Sciences

How to Cite

BRINE SHRIMP LETHALITY ASSAY AND LARVICIDAL ACTIVITY OF N-HEPTADECYL-N-(8-OCTA-1, 3-DIENE-6-YNYL)AMINE (C25H44N) ISOLATED FROM COMBRETUM MOLLE R. (2021). FUDMA JOURNAL OF SCIENCES, 5(2), 166-172. https://doi.org/10.33003/fjs-2021-0502-636

Most read articles by the same author(s)