PRETREATMENT OF MILLET HUSK USING ALKALINE HYDROGEN PEROXIDE TO ENHANCE ENZYMATIC HYDROLYSIS FOR REDUCING SUGAR PRODUCTION

  • Zeenat Ibrahim Saulawa
  • Lawal Nura
  • Muntari Bala
  • Abdullahi A. Iman
Keywords: pretreatment, cellulose, hydrolysis, optimization, millet husk, hydrogen peroxide

Abstract

The effectiveness of alkaline hydrogen peroxide as a suitable choice of pretreatment for the conversion of millet husk to reducing sugars using cellulase enzyme for hydrolysis and subsequent ethanol production was determined. The effects of three variables on reducing sugar production from millet husk were determined using one factor at a time (OFAT) method namely; peroxide concentration, pretreatment time and pretreatment temperature. From the results, it was observed that a significant (P<0.05) amount of reducing sugars were lost during pretreatment of millet husk. The untreated group which was only physically pretreated (milled) however yielded a significantly higher (P<0.05) reducing sugar concentration of 10.67mg/ml after enzymatic hydrolysis while the highest reducing sugar concentration of 4.82mg/ml was obtained using 0.375%v/v peroxide concentration for 60minutes at 250C. Therefore, pretreatment of biomass with alkaline hydrogen peroxide may be more suitable for feedstock with high lignin contents than millet husk.

References

Diaz, A., Toullec, J. L., Blandino, A., Ory, I. D. and Caro, I. (2013). Pretreatment of rice hulls with alkaline peroxide to enhance enzyme hydrolysis for ethanol production. Chemical Engineering Transactions 32,949-954.

Dutra, E. D., Santos, F. A., Alencar, B. R. A., Reis, A. L. S., Fatima, R. S., Katia, A. S., Marcos, A. M. and Menezes, R. S. C. (2017). Alkaline hydrogen peroxide pretreatment of lignocellulosic biomass: status and perspectives. Biomass Conv. Bioref.

El-Naggar, A. N., Deraz, S. and Khalil, A. (2014). Bioethanol production from lignocellulosic feed stocks based on enzymatic hydrolysis: Current status and recent developments. Biotechnology.

Hernawan, R., Maryana, D., Pratiwi, S. and Rosyida, V. T. (2017). Bioethanol production from sugarcane bagasse by simultaneous saccharification and fermentation using Saccharomyces cerevisiae. International conference on Chemistry, Chemical process and Engineering. AIP conference proceedings 1823.

Juarez, J. M., Hernando, A. L., Torre, R. M., Lanza, S.B., Rodriguez, S.B. (2016). Saccharification of microalgae biomass obtained from waste water treatment by enzymatic hydrolysis. Effect of alkaline peroxide pretreatment. Bioresour Technol 104:349-357.

Karagoz, P. and Ozkan, M. (2014). Ethanol production from wheat straw by Saccharomyces cerevisiae and Scheffersomyces stipitis co culture in batch and continuous system. Bioresource Technology, 158, 286-293.

Kim, J.S, Lee, Y.Y and Kim, T.H. (2016). A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresorce Technology 199:42–48.

Li, J. L., Xia, W.J., Ma, G.P., Chen, Y. L. and Ma, Y.Y. (2019). A study on the enzymatic properties and reuse of cellulase immobilized with carbon nano tubes and sodium alginate, AMB Express, 9:122.

Miller G. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar, analytical chemistry 31, Pp. 426-428.

Mohd, A., Abdullah, R., Jambo,A., Marbawi, H., Gansau, J. A., Faik, M. A. and Rodrigues, F. K. (2017). Yeasts in sustainable bioethanol production. Biochemistry and Biophysics Reports, 10: 52-61.

Rabelo, S. C., Amezquita, N. A., Andrade, R.R., Maciel, F. R. and Costa, A. C. (2011). Ethanol
production from enzymatic hydrolysis of sugarcane bagase pretreated with lime and alkaline hydrogen peroxide. Biomass bioenergy, 35: 2600-2607.

Saha, B. C. and Cotta M. A. (2006). Ethanol production from alkaline peroxide pretreated enzymatically saccharified wheat straw. Biotechnol Prog (22): 449-453.

Saha, B. C. and Cotta, M. A. (2007). Enzymatic saccharification and fermentation of alkaline peroxide pretreated rice hulls to ethanol. Enzym Microb Technol 41: 528-532.

Santos, F.A., Queiróz, J.H., Colodette, J.L., Manfredi, M., Queiroz, M., Caldas, C.S. and Soares, F.E.F. (2014) Otimização do pré-tratamento hidrotérmico da palha de cana-de-açúcar visando à produção de etanol celulósico. Quim Nova 37:56–62.

Sun, R. C., Fang, J. M. and Tomkinson, J. (2000). Delignification of Rye straw using hydrogen peroxide. Ind Crop Prod 12: 71-83.
Published
2021-07-06
How to Cite
Saulawa, Z. I., Nura, L., Bala, M., & Iman, A. A. (2021). PRETREATMENT OF MILLET HUSK USING ALKALINE HYDROGEN PEROXIDE TO ENHANCE ENZYMATIC HYDROLYSIS FOR REDUCING SUGAR PRODUCTION. FUDMA JOURNAL OF SCIENCES, 5(2), 289 - 294. https://doi.org/10.33003/fjs-2021-0502-617