IN VITRO EVALUATION OF ANISOPUS MANNII AND LEPTADENIA HASTATA FOR ANTIBACTERIAL POTENTIAL AGAINST BACTERIAL ISOLATES FROM DIABETIC WOUND

  • Sakina B. Sani
  • B. M. Usman
  • M. Hayatu
  • A. Y. Hardiyya
  • A. Bilkisu
  • M. Umma
  • M. M. Mamadina
  • M. I. Hassan
  • I. Safiyanu
  • A. Jibril
  • Y. S. Ajingi
  • A. I. Sale
Keywords: Antibacterial, Asclepeadeceae, Anisopus mannii, Leptadenia hastate, phytochemical, diabetic wound

Abstract

Leptadenia hastata and Anisopus mannii – are perennial plants of family Asclepiadaceae. They are widely distributed in West Africa and are locally used as anti-diabetic agents in Northern Nigeria. This study was conducted to investigate the phytochemical constituent and antibacterial activity of the crude ethanol extract of the Leptadenia hastata and Anisopus mannii against some bacterial isolates from diabetic wound. The phytochemical screening was carried out using standard protocol and antibacterial activity was determined by agar well diffusion method followed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) on the plant extract that showed activity. Result of phytochemical screening reveals the present of tannins, phenols, flavonols, saponins, and alkaloids in all the plants extract except in Anisopus mannii where alkaloid is absent. Also, result from antibacterial activity of leptadenia hastata demonstrated inhibition zones ranging from 11.5±0.71 to 20.0±0.00 at various concentrations (8mg/ml and 4mg/ml) against the tested organisms (Citrobacter specie, E.coli, Proteus vulgaris.) with MIC and MBC values ranging from 2mg/ml-8mg/ml. Hence, the ethanol extract of Leptadenia hastata can be considered as new therapeutic agent for the treatment of diabetic wound infection. Further studies need to be carried out to investigate toxicological effect and diabetic wound healing property of the plant.

References

Agyare C., Duah B.Y., Bekoe,E.P., Hensel, A,, Dapaah,S.O. and Appiah, T. (2016). Review: African medicinal plants with wound healing properties. Journal of Ethnopharmacology, 177: 85–100.

Agyare, C., Kisseih, E., Kyere, I.Y. and Sampane, O.P.P. (2014). Medicinal plants used in wound care: Assessment of wound healing and antimicrobial properties of Zanthoxylum leprieurii. Biological Sciences and Pharceutical Research, 2(8):081-089.

Alam,G., Singh, M.P. Singh A. (2011). Wound healing potential of some medicinal plants. International Journal of Pharmaceutical Sciences Review and Research 9(1):136-145

Ali, M., Diso, S.U., Minjibir, A.A., Sani, J.A., Nas, .FS., Yahya, A. (2017). Assessment of medicinal plants used in treatment of various ailments in Kano city Nigeria. Asian J. Biores. Technol. 2(4):1-11.

Ali, M., Imam, I.U., Abdallah, M.S. (2019). Antibacterial Activity of Leptadenia Hastata Leaves Extracts against Some Gastro-Intestinal Isolates. Archives in Biomedical Engineering & Biotechnology. 1(3):1-5.

Aliero,A.A. and Wara, S.H. (2009). Validating the medicinal potential of Leptadenia hastata. African Journal of Pharmacy and Pharmacology, 3(6): 335-338.

Aliyu, A.B., Ibrahim,H., Musa, A.M., Ibrahim, M.A., Oyewale, A.O. and Amupitan, J.O. (2010). In vitro evaluation of antioxidant activity of African Journal of Biotechnology, 9(16);2437-2441.

Bader, M. (2008). Diabetic foot infection. American Academy of Family Physicians, 78(1):71-79

Cassidy, A., Hanley, B., Lamuela-Raventos, R.M. (2000). Isoflavones, lignans and stilbenes-origins, metabolism and potential importance to human health. Journal of the Science of Food and Agriculture. 80: 1044-1062.

Danbatta, S.H. and Aliyu, B.S. (2011). A survey of major ethno medicinal plants of Kano North, Nigeria, their knowledge and uses by traditional healers. Bayero Journal of Pure and Applied Sciences. 4(2):28-35

Dholi, S.K., Raparla, R., Mankala, S.K., Nagappan, K. (2011). Invivo Antidiabetic evaluation of Neem leaf extract in alloxan induced rats. Journal of Applied Pharmaceutical Science, 1 : 100-105

Edeoga, H.O., Okwu, D.E. and Mbaebie, B.O. (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, 4:685 – 688

Eloff, J.N. (1998). Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol., 60(1):1-8

Haruna, A., Mann, A., Ogbadoyi, E.O. (2017). Phytochemical composition and antitrypanosomal activity of leaf extract of Leptadenia hastata(Pers) Decne. Bayero Journal of Pure and Applied Sciences. 10(2):292-299.

Inngjerdingen, K., Inngjerdingen, K., Nergård, C.S., Diallo, D., Mounkoro, P.P. and Paulsen, B.S. (2004). An ethnopharmacological survey of plants used for wound healing in Dogonland, Mali. West Africa. Journal of Ethnopharmacology, 92: 233–244.

Kang, N.J., Shin, S.H., Lee, H.J., Lee, K.W. (2011). Polyphenols as small molecular inhibitors of signaling cascades in carcinogenesis. Pharmacology & Therapeutics. 130: 310-324.

Kankara, S.S., Ibrahim, M.H., Mustafa, M., Go, R. (2015). Ethnobotanical survey of medicinal plants used for traditional maternal healthcare in Katsina state, Nigeria. S. Afr. J. Bot. 97: 165-175.

Kothari, V.,Gupta, A. and Naraniwal, M. (2012). Extraction methods for preparation of bioactive plants extract; a comparative study. LAP LAMBERT academic publication.

Kumar, A.S. (2016). Medicinal plants: Future source of new drugs. International Journal of Herbal Medicine. 4(4): 59-64.

Lakshmi, S.S., Chelladurai, G. and Suresh, B. (2016). In vitro studies on medicinal plants used against bacterial diabetic foot ulcer (BDFU) and urinary tract infected (UTI) causing pathogens. J Parasit Dis. 40(3):667–673.

Lar, P.M., Ojile, E.E., Dashe, E. and Oluama, J.N. (2011). Antibacterial activity of Moringa olefera seed extracts on some gram negative bacteria isolates. African Journal of Natural sciences. 14: 57-52.

Mann, A., Yahya Y., Banson A. and Ajayi G.O. (2008). Phytochemical and antibacterial screening of Anogeissus leiocarpus against some microorganisms associated with infectious wound. African Journal of Microbiology Research, 2:060-062.

Negbenebor, H.E., Shehu, K., Mukhtar, M.F., Oiza, A.Z., Nura, S. and Fagwalawa, L.D. (2017). Ethno Botanical Survey of Medicinal Plants Used by Hausa People in the Management of Diabetes Mellitus in Kano Metropolis, Northern Nigeria. European Journal of Medicinal Plants. 18(2):1-10.

Nuhu, A.A. And Odinaka, U.F. (2012). Phtochemical creanng and antimcrobal activity of Sacocephalus pobeguini L. Africican Journal of Pharmaceutical research and development, 9(1):86-92

Nyamai, D.W., Arika, W., Ogola, P.E., Njagi, E.N.M, Ngugi, M.P. (2016). Medicinally Important Phytochemicals: An Untapped Research Avenue. Journal of pharmacognosy and phytochemistry 4(1):35-46.

Nyamai, D.W., Mawia, A.M., Wambua, F.K., Njoroge, A. and Matheri, F. (2015). Phytochemical Profile of Prunus africanaStem Bark from Kenya. Journal of Pharmacognosy & Natural Products. 2015; 1: 110.

Oguntibeju, O.O. (2019). Medicinal plants and their effects on diabetic wound healing, Veterinary World, 12(5): 653-663.

Oludare, O.T., Olufunke, A., Desoji,C.A. and Joshua, A. (2016). Antimicrobial activity and phytochemical composition of crude ethyl acetate extracts of Anisopus mannii on some selected clinically important microorganisms. International Journal of Pharma Sciences and Research. 7(5):231-139.

Olusola, A., Olutayo O., Michael A. and Adepoju A.J. (2012). Antimicrobial activity and Elemental analysis of Cassia siberiana leaves Using Atomic Absorption Spectrometer . J. Nat. Prod. Plant Resour.2 (1):9-18.

Osibemhe, M., Lawal, N., Umar, D., Omaji, G.O. and Jibiya, S.A. (2017). Effect of aqueous extract of Anisopus manni stem in Alloxan-induced diabetic rats. Science World Journal 12(3):7-8.

Rao, N., and Lipsky, B.A. (2007). Optimising antimicrobial therapy in diabetic foot infections. Drugs. 67:195-214.

Ros,E. (2000). Intestinal absorption of triglyceride and cholesterol. Dietary and pharmacological inhibition to reduce cardiovascular risk. Atherosclerosis. 151: 357-379.

Roy, P., Abdulsalam, F.I., Pandey, D.K., Bhattacharjee, A. , Reddy, N.E. and Malik, T. (2015). Evaluation of antioxidant, antibacterial, and antidiabetic potential of two traditional medicinal plants of India: Swertia cordata and Swertia chirayita. Pharmacognosy Research. 7(1):S57-S62.

Sani, D., Sanni, S. and Ngulde, S.I. (2009). Phytochemical and antimicrobial screening of the stem aqueous extract of Anisopus mannii. Journal of Medicinal Plants Research. 3(3):112-115.

Sani, S.B., Aliyu, B.S., Haruna, M., Yahya, S.M., Mardiyya, A.Y., Ajingi, Y.S., Wada, L.H. and Abba, H. (2019). Local plants and diabetes management: folkloric practices in metropolitan Kano, Nigeria. Bayero Journal of Pure and Applied Sciences. 12(1):268-273.

Shinkafi, T.S, Bello L, Hassan, S.W, Ali, S. (2015). An ethnobotanical survey of antidiabetic plants used by Hausa-Fulani tribes in Sokoto, Northwest Nigeria. J. Ethnopharmacol. 172(22), 91-99.

Suresh, A., Muthu,G., Suresh, G., Premnath, R., Gopinath, P., Moses, A. and Ramesh, S. (2012). Screening of antibacterial properties of indian medicinal plants against multi drug resistant diabetic foot ulcer isolates. International Journal of Phytopharmacology. 3(2): 139-146.

Swargiary, A. (2017):Recent trends in traditionally used medicinal plants and drug discovery. Asian Journal of Pharmacy and Pharmacology. 3(4):

Taylor, L. N.D. (2000). Plant Based Drugs and Medicines, Rain Tree Nutrition

Thomas, S.D. (2012). Leptadenia hastata: A Review of its Traditional uses and its Pharmacological Activity. Medicinal chemistry. 2(7):148-150.

Umaru, I.J., Badruddin,F.A., Assim, Z.B.and Umaru, H.A. (2018). Antimicrobial properties of leptadenia hastata (pers) decne leaves extract. International Journal of Pharmacy and Pharmaceutical Sciences. 10(2):149-152.

Varma N. (2016). Phytoconstituents and Their Mode of Extractions: An Overview. Research Journal of Chemical and Environmental Sciences, 4(2):8-15.

Yusha’u, M. (2011). Phytochemical screening and antibacterial activity of Hibiscus sabdariffa extracts against some urinary tract isolates. Best journal. 8(2):83-86.
Published
2021-07-01
How to Cite
Sani, S. B., Usman, B. M., Hayatu, M., Hardiyya, A. Y., Bilkisu, A., Umma, M., Mamadina, M. M., Hassan, M. I., Safiyanu, I., Jibril, A., Ajingi, Y. S., & Sale, A. I. (2021). IN VITRO EVALUATION OF ANISOPUS MANNII AND LEPTADENIA HASTATA FOR ANTIBACTERIAL POTENTIAL AGAINST BACTERIAL ISOLATES FROM DIABETIC WOUND. FUDMA JOURNAL OF SCIENCES, 5(2), 109 - 114. https://doi.org/10.33003/fjs-2021-0501-601

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