PHYTOCHEMICAL ANALYSIS AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF TOKAR SHA AGAINST ENTERIC BACTERIA
Abstract
The antibacterial activity of Tokar sha; a local traditional medication widely used by many people in North-west zone of Nigeria especially Sokoto, Kebbi and Zamfara against enteric infections were examined against some clinical isolates of pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Bacillus cereus and Salmonella typhi) using agar well diffusion method. The pattern of inhibition varied with the tokar sha concentrations and the organisms tested. The tokar sha was more effective on E. coli with a maximum zone of growth inhibition of 25mm at 35mg/ml followed by B. cereus (20mm). However, S. aureus and S. typhi were resistant to tokar sha at all concentrations tested. The minimum inhibitory concentrations (MIC) were found to be 35mg/ml for both E. coli and B. cereus. The antibacterial activities exhibited by tokar sha in this study could be attributed to the presence of its constituents which signifies the potential of the tokar sha as a therapeutic agent. These findings may justify the ethnomedicinal use of tokar sha as an antibacterial agent against enterobacteria
References
Adebolu, T. T. and Oladimeji, S. A. (2005). Antimicrobial Activity of Leaf Extracts of Ocimum Gratissimum on Selected Diarrhoea Causing Bacteria in Southwestern Nigeriaâ€. African Journal of Biotechnology 4 :(7) 682-684.
Arabski, M., Wasik, S., Dworecki, K. and Kaca, W. (2009). Laser interferometric and cultivation methods for measurement of colistin/ampicilin and saponin interactions with smooth and rough of Proteus mirabilis lipopolysaccharides and cells. Journal of Microbiological Methods, vol. 77, no. 2, pp. 178–183.
Arora (2004). Textbook of Microbiology. Satish Kumar publishers, India.
Babayi, H., Kolo, I., Okogun, J. I. and Ijah, U. J. (2004). The Antimicrobial Activities of Methanolic Extracts of Eucalyptus Camaldunensis and Terminalia Catappa Against Some Pathogenic Microorganisms. Biokemistri; 16(2):106-111.
Boakye-Yiadom (1979). Antimicrobial Properties of Some West African Medicinal Plants and Antimicrobial Activity of Aqueous Extract of Cryptolepsis Sangumolenta. Quarterly Journal of Crude Drug Research, 17(2):78-80.
Boyanova, L. (2005). Activity of Bulgaris Propolis against 94 Helicobacter pylori strains in Vitro by Agar-Well Diffusion, Agar Dilution and Disc Diffusion Methods. Journal of Medical Microbiology, 54:481-83.
Brain, K. and Turner, T., (1975). The Practical Evaluation of Phytopharmaceuticals. Great Britain: John Wrightan and Sons Ltd, P. 2.
Buck, W.B., Osweiter, G.D. and Van Gelder, A. G. (1976). Clinical and diagnostic Veterinary Toxicology. Iowa: Kendall/hunt Publishing Co; P.5211.
Cohen, M. L. (1992). Epidemiology of Drug Resistanceâ€. Implications for a Post-Antimicrobial Era. Science, 257 :( 5073) 1050-1055.
Cowan, M. M. (1999). Plants Products as Antimicrobial Agents. Clinical Microbiology Review. 12:564-582.
Elvin-Lewis, M. (2001). Should we be Concerned about Herbal Remedies. Journal of Ethno pharmacology, 75 :( 2-3) 141-164.
Erdogrul. O. T. (2002). Antibacterial Activity of Some Plant Extracts Used in Folk Medicine. Pharmaceutical Biology; 40:269-273.
Kabara, J. J. (1978). Health Oils from the Tree of Life, In Pharmacological Effects of Lipids. AOCS Press London. pp. 624-629.
Kafaru, E. (1994). Immense Help from Nature`s Workshop. Elika Health Services Ltd., Academic Press Plc. Lagos, Nigeria: 1-27.
Naqvi, S. A. H., Khan, M.S.Y. and Vohora, S. B. (1991). Antibacterial, Antifungal and Anthelmintic Investigations on Indian Medicinal Plants (Fitoterapia). Journal of Indian Medicine,6 :( 2) 221-228.
Nascimento, G. G. F., Freitas, J. P. C. and Silva, G.L. (2000). Antibacterial Activity of Plant Extracts and Phytochemicals on Antibiotic-Resistant Bacteriaâ€. Brazilian Journal of Microbiology 31: 247-256.
NCCLS, (2000). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Stardard. 5th Ed. Wayne, PA USA.
Osowole, O., Ajaiyeoba, E., Bolaji, O., Akinboye, D., Fawole, O., Gbotosho, G. and Oduola, A. A. (2005). Survey of Treatment Practices for Febrile Illnesses among Traditional Healers in The Nigerian Middle Belt Zone. African Journal of Traditional and Complimentary/ Alternative Medicine. 2:337–34412.
Owolabi, O. J., Omogbai, E. K. I. and Obasuyi, O. (2007). Antifungal and Antibacterial Activities of the Ethanolic and Aqueous Extract of Kigella Africana (Bignoniaceae) Stem Bark. African Journal of Biotechnology, 6 :( 14) 1677-1680.
Rios, J. L. and Recio, M. C. (2005). Medicinal Plants and Antimicrobial Activity. Journal of Ethno pharmacology. 100: 80-84.
Rodders, J., Speedie, M., Tyler, V. (1996). Pharmacognosy and Pharmaco-Biotecknology. Baltimore: Williams and Wilkins.7-12.
Shibata, H., Kondo, K., Katsuyama, R., Kawazoe, K., Sato, Y., Murakami, K., Takaishi, Y., Arakaki, N. and Higuti, T. (2005). Alkyl Gallates, Intensifiers of ß-Lactam Susceptibility in Methicillin-Resistant Staphylococcus Aureus Antimicrobial Agents Chemotherapy. 49(2):549-555.
Sofowora, E. A. (1993). Medicinal plants and traditional remedies in Africa. University of Ife Press, Nigeria.
Trease, G. E. and Evans, W.C. (2009). Textbook of Pharmacognosy, 16th (Ed) BalliersTindall, London.
Ugbogu, O. C. (2006). Lauric Acid Content and Inhibitory Effect of Palm Kernel Oil on Two Bacterial Isolates and C. Albicans. African Journal of Biotechnology 5(11):1045-1047.
Yadesa, T.M., Gudina, E.K. and Angamo, M.T. (2015). Antimicrobial Use-Related Problems and Predictors among Hospitalized Medical In-Patients in Southwest Ethiopia: Prospective Observational Study. PLoS ONE 10(12): e0138385. doi:10.1371/journal. pone.013838
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