MORPHOLOGICAL AND MOLECULAR IDENTIFICATION of ENDOPHYTIC FUNGI FROM PSIDIUM Guajava and Vachellia nilotica
DOI:
https://doi.org/10.33003/fjs-2026-1005-4912Keywords:
Endophytic fungi, Medicinal plants, Morphological, Molecular and Phylogenetic Identification, diversityAbstract
Endophytic fungi are essential components of plant ecosystems, often contributing to host health and secondary metabolites production. The present study aimed to isolate and identify fungal endophytes from the stems and leaves of P. guajava and V. nilotica. Morphological, Molecular and Phylogenetic Identification of were carried out using standard procedures. Fungi were isolated and categorized by host tissue using appropriate fungal atlas. Molecular identification was performed using rRNA sequence analysis (ITS1, 5.8S, ITS2), and phylogenetic relationships were established by comparing sequences against GenBank database matches. A total of 20 endophytic fungi were isolated from four host segments (P. guajava stem (PGS), P. guajava leaves, (PGL), V. nilotica leaves (VNL), V. nilotica stem (VNS) corresponding to a colonization frequency of 80.95%. The following fungal isolates were identified: Thysanophora penicilloides, Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Fusarium spp, Sporangia spp, Fusarium verticillioides, Aureobasidium pullulans, and Gliocladium roseum. Psidium guajava leaves (PGL) exhibited the highest colonization (26.19%), followed by its stem (23.80%), while Vachellia nilotica showed lower frequencies (16.66% for leaves and 14.28% for stems). Dominant species identified include: Aspergillus fumigatus, Fusarium verticillioides, Aspergillus flavus, and Aspergillus niger. Molecular analysis of the ITS/rRNA regions confirmed the identity of four primary isolates (PGL, VNL, PGS, VNS) with DNA fragments ranging from 558 to 1056 bp. The DNA fragments show a sequence identity percentages range between 99.77% and 99.99% indicating a high taxonomic accuracy within the respective species clades.
References
Gao, Y., Xu,Y., Dong, Z., Guo,Y., Luo, J., Wang, F., Yan, L., Zou, X. (2025). Endophytic Fungal Diversity and Its Interaction Mechanism with Medicinal Plants. Molecules, 30(1):1028 -1034.
Munir, E.; Yurnaliza; Lutfia, A.; Hartanto, A. (2022). Isolation and Characterization of Phosphate Solubilizing Activity of Endophytic Fungi from Zingiberaceous Species. OnLine J. Biol. Sci., 22, 149–156.
Li, X.; Awais, M.; Wang, S.; Zhang, Z.; Zhao, S.; Liu, Y.; Sun, Z.; Fu, H.; Li, T. (2025). The process of nitrogen-adaptation root endophytic bacterial rather than phosphorus-adaptation fungal subcommunities construction unveiled the tomato yield improvement under long-term fertilization. Front. Microbiol. 15, 1487323
Chancellor, T.; Smith, D.P.; Chen, W.; Clark, S.J.; Venter, E.; Halsey, K.; Carrera, E.; Mcmillan, V.; Canning, G.; Armer, V.J. A. (2024). fungal endophyte induces local cell wall-mediated resistance in wheat roots against take-all disease. Front. Plant Sci., 15, 1444271
Bora, P and Devi, N.N. (2023). Exploration of the chemical constituents and its antioxidant, antibacterial activities of endophytic fungi isolated from the medicinal plant Dillenia indica. Arch. Microbiol., 205 -214.
Li, N., Xu, D., Huang, R. H., Zheng, J.Y., Liu, Y.Y., Hu, B. S., Gu, Y.Q and Du, Q. (2022). A New Source of Diterpene Lactones from Andrographis paniculata (Burm. f.) Nees-Two Endophytic Fungi of Colletotrichum sp. with Antibacterial and Antioxidant Activities. Front. Microbiol., 13(1): 819770.
Pandy, R.; Kumar, S.S.; Suresh, P.; Annaraj, J.; Pandi, M.; Vellasamy, S.; Sagadevan, S. (2023). Screening and characterization of fungal taxol-producing endophytic fungi for evaluation of antimicrobial and anticancer activities. Open Chem., 21, 20220344
Utami, S. H., Dwi, R., Rai, Y.S. Sugi, H., Chomisatut, T., Fitri M. And Faiza, N. I. N. (2020). Antimicrobial activity of endophytic fungi isolated from physalisangulata L plant. Journal of Energy and Natural Resources, 9 (1): 10-13.
World Health Organization (2020). Applied microbiology: Rhodesian human African trypanosomiasis (rHAT) in Kafue Nation Park, Zambia-David square Zambia Hokkaido University. Journal of food and microbiology, (4),1-12.
Harry, P. and Koning, D. (2020). The drugs of sleeping sickness: Their mechanism of action and resistance and a brief history. Journal of tropical medicine and infectious diseases, 514- 23.
Sergio, M., Lilia C., Sarah H., Elizabeth A., Carmenza S., Alicia I., William H., and Luis C. (2021). Screening and evaluation of anti-parasitic and In vitro anticancer activity of Panamanian and entophytic fungi. Journal of Marine Drugs, 11(10), 4058-4082.
Hussaina, S. B., Faruk, S. N., Maigari, A.K., and Salisu, A. (2025). The in -vitro activity of an extract derived from selected Endophytic fungal species isolated from Psidium guajava against Trypanosoma Brucei Brucei. International Research Journal of Biological Sciences, 14(1): 1-7.
Afolayan, A. J., et al. (2020). "Phytochemical analysis and antimicrobial activity of Psidium guajava leaf extracts." Journal of Pharmacy and Pharmacology, 72(8), 1038-1046.
Desalegn, B., et al. (2020). "Endophytic fungi: A treasure trove of bioactive compounds." Journal of Fungi, 6(3), 247.
Emiru, A.Y., Makonnen, E., Regassa, F., Regassa, F. and Tufa, T.B. (2021). Antitrypanosomal activity of hydromethanol extract of leaves of Cymbopogon citratus and seeds of Lepidium sativum: in-vivo mice model. BMC Complementary Medicine and Therapies; 21:290.
Hussaini, I.M., Ibrahim, S., Usman, A. and Musa, B. (2024). Antibacterial Potential of Endophytic Fungi Isolated from Psidium guajava (Guava) Leaf against Escherichia coli and Klebsiella pneumonia. UJMR, Conference Special Issue. 9(3):180 – 186.
Digra, S. and Nonzom, S. (2023). An insight into endophytic antimicrobial compounds: An updated analysis. Plant Biotechnology Report, 1–31.
Gupta, A., Meshram, V., Gupta, M., Goyal, S., Qureshi, K.A., Jaremko, M. and Shukla, K.K. (2023). Fungal Endophytes: Microfactories of Novel Bioactive Compounds with Therapeutic Interventions; A Comprehensive Review on the Biotechnological Developments in the Field of Fungal Endophytic Biology over the Last Decade. Biomolecules, 13, 1038.
Eric, N. T., Sandrine, A. Y. F., Bruno, L. N., Florentina, M and Maximilienne, A. N. (2020). Diversity of Endophytic Fungi of Psidium guajava (Myrtaceae) and Their Antagonistic Activity against Two Banana Pathogens. Journal of Advances in Microbiology, 20(11): 86-10.
Meshram, V., Elazar, M., Maymon, M., Sharma, G., Shawahna, R., Charuvi, D. and Freeman, S. (2023). Endophytic Fusarium clavum confers growth and salt tolerance in Cucumismelo. Environmental and Experimental Botany, 206: 105–153.
Tchamgoue, E.N., Fanche, S.A.Y., Ndjakou, B.L., Matei, F. and Nyegue, M.A. (2020). Diversity of Endophytic Fungi of Psidium guajava (Myrtaceae) and Their Antagonistic Activity against Two Banana Pathogens. 20(11): 86-101.
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Copyright (c) 2026 Adamu Babayo Samaila, Sam Mao Panda, Auwal AAlhassan Barde, Sani Tajo Tukur, Aishatu Adamu Samaila
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