DIVERSITY OF MICROORGANISMS IN BIOGAS PRODUCTION THROUGH CO-DIGESTION OF CATTLE DUNG, POULTRY WASTE, AND PALM OIL MILL EFFLUENT

Authors

  • Dr. (Mrs). Oluwaponmile Tosin Ojetokun
    Federal University of Agriculture, Abeokuta
  • Prof. Babatunde Saheed Bada
    Federal University of Agriculture, Abeokuta
  • Dr. (Mrs). Adijat Olabisi Atayese
    Federal University of Agriculture, Abeokuta
  • Dr. Olumide Joshua Ojetokun
    Ahmadu Bello University image/svg+xml

Abstract

This study assessed microbial diversity in biogas production from co-digestion of cattle dung, poultry waste, and palm oil mill effluent (POME). The experiment used a batch feeding system with 28-day retention, examining six substrate treatments: (CD), (PW), (CD:PW 1:1), (CD: POME 1:1), (PW: POME 1:1), and (CD: PW: POME 1:1:2). pH and temperature were monitored using a pH/temperature meter, and biogas volume was measured by water displacement. The data underwent descriptive and inferential statistical analyses. The optimal co-digestion treatment of PW: POME (1:1) yielded the highest cumulative biogas volume (8.33 dm³) and peak production of 3.80 dm³ on day 14. Initial substrate analysis showed total viable bacterial counts of 6.35 × 105, 2.17 × 105, and 0.27 × 105 CFU/mL for CD, PW, and POME, respectively, while total viable fungal counts were 2.05 × 105, 3.25 × 105, and 3.05 × 105 CFU/mL. Microbial analysis identified four suspected bacterial species (Bacillus aquimaris, B. cibi, B. altitudinis, and B. cereus), five fungal species (Curvularia lunata, Aspergillus flavus, A. nidulans, A. aculeatus, and Trichoderma viride), and two yeast species (Candida albicans and C. tropicalis), with Bacillus and Aspergillus being dominant genera. The biogas slurry is rich in nutrients, particularly potassium, indicating potential as a biofertilizer. The co-digestion process promoted a diverse microbial community dominated by Bacillus and Aspergillus species, which enhance substrate breakdown and biogas production.

Author Biographies

Dr. (Mrs). Oluwaponmile Tosin Ojetokun

Institute of Food Security, Environmental Resources, and Agricultural Research

Research Fellow II

Prof. Babatunde Saheed Bada

Department of Environmental Management and Toxicology, College of Environmental Resources Management, 

Professor

Dr. (Mrs). Adijat Olabisi Atayese

Center of Excellence in Agricultural Development and Sustainable Environment

Research Fellow I

Dr. Olumide Joshua Ojetokun

Department of Community Medicine

Masters Student

Dimensions

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Published

29-12-2025

How to Cite

Ojetokun, O., Bada, B., Atayese, A. O., & Ojetokun, O. J. (2025). DIVERSITY OF MICROORGANISMS IN BIOGAS PRODUCTION THROUGH CO-DIGESTION OF CATTLE DUNG, POULTRY WASTE, AND PALM OIL MILL EFFLUENT. FUDMA JOURNAL OF SCIENCES, 9(12), 369-375. https://doi.org/10.33003/fjs-2025-0912-4173

Issue

Vol. 9 No. 12 (2025): FUDMA Journal of Sciences - Vol. 9 No. 12

Section

Research Articles
Copyright & Licensing

Copyright (c) 2025 Dr. (Mrs). Oluwaponmile Tosin Ojetokun, Prof. Babatunde Saheed Bada, Dr. (Mrs). Adijat Olabisi Atayese, Dr. Olumide Joshua Ojetokun

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Ojetokun, O., Bada, B., Atayese, A. O., & Ojetokun, O. J. (2025). DIVERSITY OF MICROORGANISMS IN BIOGAS PRODUCTION THROUGH CO-DIGESTION OF CATTLE DUNG, POULTRY WASTE, AND PALM OIL MILL EFFLUENT. FUDMA JOURNAL OF SCIENCES, 9(12), 369-375. https://doi.org/10.33003/fjs-2025-0912-4173