PHYTOCHEMICAL PROFILING, ANTIOXIDANT POTENTIAL, AND LC-MS ANALYSIS OF BIOACTIVE COMPOUNDS IN Ipomoea batatas (L.) LAM LEAVES
DOI:
https://doi.org/10.33003/fjs-2025-0906-3755Keywords:
Ipomoea batatas, Phytochemicals, Antioxidant activity, LC-MS, Total phenolic content, Total flavonoid contentAbstract
Leaves of Ipomoea batatas (sweet potato) are abundant sources of phytochemicals with possible therapeutic benefits. This study investigated the phytochemical profile, total phenolic content (TPC) and Total flavonoid content (TFC), antioxidant activity, and bioactive compounds in ethanol extracts of I. batatas leaves using qualitative and quantitative methods. The phytochemical screening revealed the presence of alkaloids, flavonoids, terpenoids, tannins, and glycosides in separate solvent fractions, namely methanol, hexane, dichloromethane, ethyl acetate, and aqueous. Quantitative analysis revealed the highest TPC (172.58 ± 2.37 mg GAE/g) in aqueous fraction and the highest TFC (59.89 ± 4.70 mg QE/g) in ethyl acetate fraction. DPPH assay showed a strong antioxidant effect using the ethyl acetate fraction, which was observed to have the lowest IC50 (18.42 ± 1.29 μg/mL), comparable to quercetin. LCMS analysis has identified up to 15 bioactive compounds such as luteolin, quercetin, kaempferol, and chlorogenic acid among others as likely contributors to the observed bioactivities. This foliage has the potential to act as a source of natural antioxidants and further support the traditional ethnomedicine use of I. batatas leaves. The extraction solvent also shows a differential effect on phytochemical yield and bioactivity, highlighting polar solvents as ideal for the extraction of phenolic and flavonoid phytochemicals.
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