OPTIMIZATION OF LIPASE PRODUCTION USING AGRO-ALLIED WASTES BY Bacillus subterraneous IN SOLID-STATE FERMENTATION

Authors

Keywords:

Lipase, Bacillus subterraneus, Solid-State Fermentation, Agro-Allied Wastes, Enzyme Optimization

Abstract

Lipases are industrially significant enzymes widely used in food, detergent, pharmaceutical, and biodiesel industries. This study investigates the optimization of lipase production by Bacillus subterraneous isolated from abattoir soil in Kurmin mashi Abattoir, Kaduna using agro-allied wastes as substrates in solid-state fermentation (SSF). Soil samples were collected and analyzed to isolate lipase-producing bacteria, which were identified through morphological, biochemical, and molecular techniques. Various agro-wastes such as wheat bran, rice bran, sugarcane peel, and groundnut husk were evaluated for their efficiency in supporting microbial growth and enzyme production. The study aimed to enhance enzyme yield by optimizing key fermentation parameters, including pH, temperature, substrate concentration, incubation time, and agitation speed. The highest lipase activity of 6.90±0.10 U/mL was achieved under optimal conditions: pH 6, temperature 40°C, inoculums level 4.0% (w/v), and incubation time 48 hours. Among the tested agro-allied wastes, groundnut shell and groundnut oil proved to be the most effective substrate for lipase induction. The enzyme was subsequently purified using ammonium sulfate precipitation, dialysis, and gel filtration chromatography. SDS-PAGE analysis confirmed the molecular weight of the purified lipase to be 25kDa. The findings suggest significant industrial potential, particularly in biodegradable detergents, biodiesel production, food processing, and pharmaceutical applications. Moreover, the utilization of agro-waste as a fermentation substrate highlights the economic and environmental sustainability of this production approach, supporting waste valorization and circular bioeconomy models.

Dimensions

Ghosh, P. K., Saxena, R. K., Gupta, R., Yadav, R. P., & Davidson, S. (2016). Microbial lipases: Production and applications. Journal of Scientific & Industrial Research, 75(6), 437-452.

Gricajeva, A., Bendikiene, V. & Kalediene, L. (2016) Lipase of Bacillus strasphericus L1: cloning, expression and characterization. Int. J Biol. Macromol, 92:96-104

Gupta, R., Gupta, N. & Rathi, P. (2004). Bacterial lipases: An overview of production purification and biochemical properties. Applied Microbiology and Biotechnology, 64(6): 763 - 781.

Hasan, F., Shah, A. A., & Hameed, A. (2006). Industrial applications of microbial lipases. Enzyme and Microbial Technology, 39(2), 235-251. https://doi.org/10.1016/j.enzmictec.2005.10.016

Ilesanmi, O.I., Adekunle, A.E., Omolaiye, J.A., Olorode, E. M. & Ogunkanmi, A.L. (2020), Isolation, optimization and molecular characterization of lipase producing bacteria from contaminated soil. Scientific African 8 Pp 1-10.

Joseph, B., Upadhyaya, S. & Ramteke, W.P. (2011). Production of cold active bacterial lipases through semisolid state fermentation using oil cakes. Enzyme Research. 11: 796407.

Krzyczkowska, J. & Kozlowska, M. (2017). Effect of oils extracted from plant seeds on the growth and lipolytic activity of Yarrowia lipolytica yeast. J. am. Oil. Chem. Soc. 94: 661 671.

Pandey, A., Soccol, C. R., Rodriguez-Leon, J. A., & Nigam, P. (1999). Solid-State Fermentation in Biotechnology: Fundamentals and Applications. Asiatech Publishers.

Rathi, P., Bradoo, S., Saxena, R. K., & Gupta, R. (2001). A thermostable lipase from Bacillus sp.: Production, partial purification, and characterization. Microbial Cell Factories, 5(1), 1-8. https://doi.org/10.1186/1475-2859-5-1

Saadatullah, I.M. Waheedullah, N.M. & Rehman, Z. (2018). Isolation identification and characterization of a lipase producing Pseudomonas Journal of Biomaler 2(2): 51 57.

Salihu, A., Alan, M., Abdulkarim H., & Salleh, H. (2012). Gas Separation Lolade Production Insight in the utilization of renewable agricultural residues resource conserve. Recycle 59: 36-44.

Sethi, B. K., Nanda, P. K., & Sahoo, S. (2016). Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus NCFT 4269. Braz. J. Microbiol. 47: 143 149.

Sharma, R., Chisti, Y., & Banerjee, U. C. (2019). Production, purification, characterization, and applications of lipases. Biotechnology Advances, 17(5), 627-662. https://doi.org/10.1016/S0734-9750(99)00064-8

Singhania, R. R., Patel, A. K., Soccol, C. R., & Pandey, A. (2009). Recent advances in solid-state fermentation. Biochemical Engineering Journal, 44(1), 13-18. https://doi.org/10.1016/j.bej.2008.10.019

Thakur V., Tewari, R. & Sharma, R. (2014) Evaluation of Production Parameters for maximum lipase production by P.Stutzeri MTC 5618 and Scale-up bioreactor.

Vakhlu, J., & Kour, A. (2006). Yeast lipases: Enzyme purification, biochemical properties, and gene cloning. Electronic Journal of Biotechnology, 9(1), 69-85. https://doi.org/10.2225/vol9-issue1-fulltext-9

Venkatesagowda, B., Ponugupaty, E., Barbosa, A.M. & Robert, F.H. (2015). Solid-State Fermentation of coconut kernel-cake as substrate for the production of lipases by the coconut kernel-associated fungus Lasiodiplodia theobromae VBE-1. Ann Microbiol 65, 129142.

Zarinviarsagh, M., Ebrahimpour, G. & Sadeghi, H. (2017). Lipase and biosurfactant from Ochrobacterum intermedium strain MZV101 isolated by washing powder for detergent application, Lipids Health Dis 16:177

Published

27-04-2025

How to Cite

OPTIMIZATION OF LIPASE PRODUCTION USING AGRO-ALLIED WASTES BY Bacillus subterraneous IN SOLID-STATE FERMENTATION. (2025). FUDMA JOURNAL OF SCIENCES, 9, 79-83. https://doi.org/10.33003/fjs-2025-09(AHBSI)-3498

Issue

Vol. 9 (2025): FUDMA Journal of Sciences - Vol. 9 April (AHB Special Issue)

Section

Research Articles
Copyright & Licensing

FUDMA Journal of Sciences

How to Cite

OPTIMIZATION OF LIPASE PRODUCTION USING AGRO-ALLIED WASTES BY Bacillus subterraneous IN SOLID-STATE FERMENTATION. (2025). FUDMA JOURNAL OF SCIENCES, 9, 79-83. https://doi.org/10.33003/fjs-2025-09(AHBSI)-3498

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