HISTOPATHOLOGY OF WATERMELON ROOT INFECTED WITH ROOT-KNOT NEMATODE (Meloidogyne spp.) IN KWARA STATE, NIGERIA
Abstract
This study was carried out to determine the feeding pattern of root-knot nematode (RKN) infecting the root of watermelon in Ilorin, Nigeria. A commonly cultivated variety, kaolak was obtained and planted in a screen house. The screenhouse experiment was set out in a Completely Randomized Design (CRD). Two treatments were used; inoculated and uninoculated seedlings. Each treatment was replicated thirty times, making a total of sixty treatment combinations. Roots from each treatment were removed and cut into 4 to 5 cm pieces. The root materials were immediately fixed in formalin-aceto-alcohol (FAA). Results of this experiment showed the disorganization of the vascular tissue in the infected root. Visible galls were observed in the roots after 29 days which revealed similarity in feeding and reproduction of root-knot nematode in parasitized plant. The study advanced our knowledge on RKN feeding pattern in watermelon and the cosmopolitan nature of the pathogen.
References
Adams, S. O., Obonyilo, B. A., & Kolawole, T. B. (2024). Contribution of agricultural output to Nigerias constant basic price GDP: A panel data regression approach. Fudma Journal of Sciences, 8(6), 118 - 127. Retrieved from https://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/2741
Akhtar, A. and Hisamuddin (2015). Studies on the histopathological responses of Vigna mungo towards Meloidogyne Incognita at different time intervals. J Plant Pathol Microbiol 6(11) 319-329. https://doi.org/10.4172/2157-7471.1000319 DOI: https://doi.org/10.4172/2157-7471.1000319
Ayoub, A and Fathulla, C. (2020). Histopathological and morphological studies of root knot nematode Meloidogyne spp. on turfgrass (Cynodon dactylon). Plant Archives. 19, 3279-3282.
Bhat, M.Y. and Hisamuddin (2010). The effect of Meloidogyne incognita on the histopathology of Momordica charantia roots. Arch Phytopathol Plant Protect. 43, 1619-1624. DOI: https://doi.org/10.1080/03235400802678162
Bird, A.F. (1962). The inducement of giant cells by Meloidogyne javanica. Nematropica8, 1-10 DOI: https://doi.org/10.1163/187529262X00927
Davis E. L., Hussey R. S., Baum T. J., Bakker J., Schots A., et al. (2000) Nematode parasitism genes. Annu Rev Phytopathol 38: 365-396. DOI: https://doi.org/10.1146/annurev.phyto.38.1.365
Daykin, M.E. and Hussey, R.S. (1985). Staining and histopathological techniques in Nematology. In: Barker, K.R., Carter C.C. & Sasser, J.N. (Eds.). Advanced Treatise on Meloidogyne Vol. II: Methodology. North Carolina University and USAID, 39-48 pp.
Derek, G., Bartlem, M., Jones, G. K. and Ulrich, Z. (2013).Vascularization and nutrient delivery at root-knot nematode feeding sites in host roots. Journal of Experimental Botany. 65(7) 17891798, https://doi.org/10.1093/jxb/ert415 DOI: https://doi.org/10.1093/jxb/ert415
Escobar, C., Barcala, M., Cabrera, J., and Fenoll, C. (2015). Overview of root-knot nematodes and giant cells in Advances in Botanical Research, Vol.73, edsC.EscobarandC. Fenoll (Oxford: Elsevier AcademicPress), 132 DOI: https://doi.org/10.1016/bs.abr.2015.01.001
Gautam, C. and Goswani, B.K. (2002). Different Combinations of Neem Cake and Carbofuran against Meloidogyne incognita on Vigna radia. International Journal of Nematology. 12, 106-110
Greco, N. and Di Vito, M. (2009). Population dynamics and damage levels. In: Perry, R.N., Moens, M. & Starr, J.L. (Eds). Root-knot nematodes. Wallingford, UK, CAB International, pp. 246-274. DOI: https://doi.org/10.1079/9781845934927.0246
Osunlola, O.S. and Fawole, B. (2015). Histopathology of sweet potato roots infected with Meloidogyne incognita .Pakistan Journal of Nematology. 33(1): 31-37 DOI: https://doi.org/10.1155/2015/725363
Wesemael, W.M., Viaene, N., and Moens, M. (2011). Root-knot nematodes (Meloidogyne spp.) in Europe. Nematology 13, 316. https://doi.org/10.1163/138855410X526831 DOI: https://doi.org/10.1163/138855410X526831
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