EVALUATION OF Pseudomonas aeruginosa ACTIVITIES OF MUNG BEAN (Vigna radiata) SEED EXTRACT

  • Aminu Usman Federal University of Agriculture Zuru, Kebbi State
  • Abdulaziz B. Kutawa Department of Plant Science and Biotechnology, Federal University Dutsin-Ma, Katsina State
  • Danladi D. Musa Department of Plant Science and Biotechnology, Federal University Dutsin-Ma, Katsina State
  • Faruq Ahmad Danmallam Department of Veterinary Microbiology, College of Veterinary Medicine, Federal University of Agriculture Zuru, Kebbi State, Nigeria
Keywords: Anti-bacterial, Extraction, Mung bean seed, Pseudomonas aeruginosa

Abstract

This research focused on exploring of mung bean seed extracts and its potential as antibacterial agents. Mung bean (Vigna radiata) is a warm-season legume originally from the Indian subcontinent, where it was domesticated around 1500 BC. It is now cultivated widely across Asia, Africa, and the Americas due to its adaptability to diverse climates and soils. They are widely recognized for their high nutritional value and medicinal properties. The study was motivated by the increasing global concern over antimicrobial resistance and the need for natural alternatives to synthetic antibacterial agents. The research began with the collection of mung bean seeds from northern Nigeria, followed by their extraction using polar (ethanol) solvent. The extraction process aimed to evaluate the antibacterial efficacy of the mung bean extracts, antibacterial assays was conducted using the agar-well diffusion method against Pseudomonas aeruginosa. The results showed that the mung bean extracts exhibited significant inhibitory effects on the bacterial pathogens, with the largest zones of inhibition observed at higher extract concentrations. The study further analyzed the statistical significance of these results using an ANOVA, which confirmed that the antibacterial activity was consistent across different concentrations, although the differences between the concentrations were not statistically significant. The study also highlighted the potential of mung beans in food preservation, given their consistent antibacterial activity across different concentrations.

References

Adetutu, A. & Segun, I. (2018). "Phytochemical constituents and antimicrobial activities of Zingiber officinale on Pseudomonas aeruginosa." *European Journal of Plant Biology*, 4(2), 49-56.

Kadir, H. A., & Al-Mahmood, S. (2015). "Antimicrobial activity of eucalyptus leaf extracts against Pseudomonas aeruginosa." *International Journal of Pharmacy and Pharmaceutical Sciences*, 7(5), 141-144.

Kumar, A., & Prakash, G. (2019). Antibacterial activity of Aloe vera against clinical strains of Pseudomonas aeruginosa. Asian Journal of Pharmaceutical and Clinical Research. 12(4): 44-48. https://doi.org/10.22159/ajpcr.2019.v12i4.30788.

Moustafa, M. H., Younis, R. E., & Al-Shahrani, I. S. (2017). Curcumin inhibits the growth of Pseudomonas aeruginosa. The Egyptian Journal of Medical Human Genetics. 18(4): 331-337. https://doi.org/10.1016/j.ejmhg.2017.07.006. DOI: https://doi.org/10.1016/j.ejmhg.2017.07.006

Nostro, A., Germano, M. P., D'Angelo, V., Marino, M., & Cannatelli, M. A. (2004). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology. 39(5): 462-466. https://doi.org/10.1111/j.1472-765X.2004.01612.x. DOI: https://doi.org/10.1111/j.1472-765X.2004.01612.x

Xie, J., Yu, W., Liu, F., Li, G., & Zeng, Y. (2015). Baicalein also displays antibacterial activity against biofilm formation of Pseudomonas aeruginosa. Journal of Microbiology and Biotechnology. 25(7): 1233-1241. https://doi.org/10.4014/jmb.1502.02030

Al-Rooqi, M. M., et al. (2022). Comparative analysis of bioactive metabolites in soybean, pea, and mung bean sprouts. Food Research International. 150: 110746. https://doi.org/10.1016/j.foodres.2021.110746 DOI: https://doi.org/10.1016/j.foodres.2021.110746

Andrews, J. M. (2001). Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy. 48(suppl_1): 5-16. DOI: https://doi.org/10.1093/jac/48.suppl_1.5

Association of Official Analytical Chemist (AOAC). (2005). Official methods of analysis (18th ed.). AOAC International

Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength, and limitation. Medicinal & Aromatic Plants 4.3: 196.

Bhat, S., et al. (2022). Understanding the antimicrobial activity of mung bean extracts: Insights from a systematic review. Food Control. 134: 108737. https://doi.org/10.1016/j.foodcont.2021.108737 DOI: https://doi.org/10.1016/j.foodcont.2021.108737

Choudhury, P. K., et al. (2023). Bioactive peptides derived from mung bean: A review of their biological activities and potential health benefits. Journal of Functional Foods, 102: 104700. https://doi.org/10.1016/j.jff.2023.104700

Clinical and Laboratory Standards Institute (CLSI). (2012). "Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement." CLSI document M100-S22.

Dos Santos et al. (2019). Chemical composition and antibacterial activity of eucalyptus oil against Pseudomonas aureofaciens. Journal of Pharmacy and Pharmacology. 71(8): 1134-1143.

Ganesan, K., & Xu, B. (2018). A critical review on phytochemical profile and health promoting effects of mung bean (Vigna radiata). Food Science and Human Wellness. 7(1): 1133. https://doi.org/10.1016/j.fshw.2017.11 DOI: https://doi.org/10.1016/j.fshw.2017.11.002

Handa, S. S., Khanuja, S. P. S., Longo, G., & Rakesh, D. D. (2008). Extraction technologies for medicinal and aromatic plants. International Centre for Science and High Technology, Trieste.

Kim et al. (2019). Antibacterial activity of ginger extract against Pseudomonas aureofaciens. Journal of Food Protection, 82(12): 2033-2040.

Kumar et al. (2018). Curcumin-mediated inhibition of quorum sensing in Pseudomonas aureofaciens. Journal of Applied Microbiology. 125(4): 931-941

Lambrides, C.J. and Godwin, I.D. (2006). Mungbean. In: Chittarajan, K., Genome Mapping and Molecular Breeding in Plants. 3: 69-90 DOI: https://doi.org/10.1007/978-3-540-34516-9_4

Liu et al. (2020). Antibacterial activity of cinnamon oil against Pseudomonas aureofaciens. Journal of Essential Oil Research. 32(3): 239-247.

Mehta, N., Rao, P., & Saini, R. (2021). Exploration of the anti-bacterial, antioxidant, and anticancer potential of the seed coat extract of mung bean (Vigna radiata L. Wilczek). Plant Archives. 21(1): 16281633. https://doi.org/10.51470/PLANTARCHIVES.2021.v21.no1.222 DOI: https://doi.org/10.51470/PLANTARCHIVES.2021.v21.no1.222

Mogotsi, K. K., (2006). Vigna radiata (L.) R. Wilczek. In: Brink, M. & Belay, G. (Editors). PROTA 1: Cereals and pulses/Craleset lgumessecs. [CD-Rom]. PROTA, Wageningen, Netherlands

Sienkiewicz et al. (2020). Antibacterial activity of thyme essential oil against Pseudomonas aureofaciens. Journal of Essential Oil Research. 32(2): 159-167.

Singh, R., et al. (2022). Review on plant-derived bioactive peptides: Biological activities and their potential utilization in food development. Food Science and Biotechnology. 31(4): 673-685. https://doi.org/10.1007/s10068-022-01068-7.

Tang, D., Dong, Y., Ren, H., Li, L., & He, C. (2014). A review of phytochemistry, metabolite changes, and medicinal uses of the common food mung bean and its sprouts (Vigna radiata). Chemistry Central Journal, 8(1), 19. https://doi.org/10.1186/1752-153X-8-4 DOI: https://doi.org/10.1186/1752-153X-8-4

Tarkhan, A. E., & Saad, S. G. (2015). "Antibacterial activity of Nigella sativa oil against clinical isolates of Pseudomonas aeruginosa. International Journal of Microbiological Research. 6(1): 22-29. https://doi.org/10.12691/ijmr-6-1-4

Yi-Shen, Z., Shuai, S., & FitzGerald, R. (2018). Mung bean proteins and peptides: Nutritional, functional and bioactive properties. Food & Nutrition Research. 62: 1-12. https://doi.org/10.29219/fnr.v62.1290 DOI: https://doi.org/10.29219/fnr.v62.1290

Zhang et al. (2020). Antibacterial activity of garlic extract against Pseudomonas aureofaciens. Journal of Food Science. 85(5): S1448-S1456.

Published
2025-04-27
How to Cite
Usman, A., Kutawa , A. B., Musa, D. D., & Danmallam, F. A. (2025). EVALUATION OF Pseudomonas aeruginosa ACTIVITIES OF MUNG BEAN (Vigna radiata) SEED EXTRACT. FUDMA JOURNAL OF SCIENCES, 9, 42 - 46. https://doi.org/10.33003/fjs-2025-09(AHBSI)-3384