QUANTIFICATION OF QUININE IN MEDICINAL PLANTS FROM EDE, NIGERIA USING HPLC
DOI:
https://doi.org/10.33003/fjs-2025-0903-3351Keywords:
Medicinal plants, Quinine, High-performance liquid chromatography, Malaria, NigeriaAbstract
Nigeria, a West African country, is rich in medicinal plants traditionally used to manage various ailments. Quinine, a well-established antimalarial drug, has been widely used as a chemotherapeutic agent for treating malaria. This study aimed to develop and validate a rapid, simple, accurate and precise high-performance liquid chromatography (HPLC) method for the quantitatively determine the quinine content in three selected medicinal plants – Azadirachtai ndica (neem), Citrus aurantii folia (lime), and Mangifera indica (mango). Cold extraction method with vortex agitator was employed to obtain plant extracts. The separation and quantification of quinine were performed using an Agilent 1260 Infinity HPLC system equipped with a diode-array detector (DAD). Separation of quinine from extract was achieved on a Phenomenex Gemini C18 column in less than 2 minutes using an isocratic mobile phase of 30% acetonitrile and 70% double-distilled water and a flow rate of 1.0 mL/min at 254 nm. The standard calibration curve obtained was found to be linear R² = 0.9975. The analytical figures of merits were also evaluated for the percentage extraction recovery of quinine was 99.5%, the intraday precision as percent relative standard deviation was 1.14, it proved excellent % RSD which is less than 2 and of limits of detection (LOD) and limits of quantification (LOQ) were found to be 0.14µg/mL and 0.42µg/mL respectively. This study showed that, with the use of HPLC, quinine levels in these plants were accurately measured, thus, the method used was highly reliable, with excellent accuracy and precision. This study helps confirm whether...
References
Canales, Nataly. A., Hansen, T. N. G., Cornett, C., Walker, K., Driver, F., Antonelli, A.. and Rnsted, N. (2020). Historical chemical annotations of Cinchona bark collections are comparable to results from current day high-pressure liquid chromatography technologies. Journal of ethnopharmacology, 249, 112375. https://doi.org/10.1016/j.jep2019.112375.Epub2019
Carrington, Y., Guo, J., Le, C. H., Fillo, A., Kwon, J., Tran, L. T., and Ehlting, J. (2018):. Evolution of a secondary metabolic pathway from primary metabolism: shikimate and quinate biosynthesis in plants. The Plant Journal, 95(5), 823-833
Cosenza, G. P., Somavilla, N. S., Fagg, C. W., and Brando, M. G. (2013): Bitter plants used as substitute of Cinchona spp.(quina) in Brazilian traditional medicine. Journal of Ethnopharmacology, 149(3), 790-796.
Costa, Carvalhol D., Costa, H.S., Albuquerque, Tania G. and Ramos F. (2015): Advances in phenolic compounds analysis of aromatic plants and their potential applications, Trends Food Science and Technology, 45 (2) (2015) 336-354. https://doi.org/10.1016/j.tifs.2015.06.009
Gessler, M., Mueller, S., & Weber, K. (2021): Role of medicinal plants in malaria treatment: A historical perspective. Malaria Journal, 20 (1), 19.
Giovane de Jesus Gomes Ribeiro, Sun Liu Rei Yan, Giuseppe Palmisano and Carsten Wrenger (2023): Plant Extracts as a Source of Natural Products with Potential Antimalarial Effects: An Update from 2018 to 2022. Pharmaceutics 2023, 15, 1638. https://doi.org/10.3390/pharmaceutics15061638
Gosetti, F., Mazzucco, E., Zampieri, D., and Gennaro, M. C. (2010): Signal suppression/enhancement in high-performance liquid chromatography tandem mass spectrometry. Journal of Chromatography A, 1217(25), 3929-3937.
Mainasara, M. M.. Aliero, B. L. Aliero, A. A and. Dahiru, S. S (2011): Phytochemical and Antibacterial Properties of Calotropis Procera (Ait) R. Br. (Sodom Apple) Fruit and Bark Extracts. International Journal of Modern Botany 2011; 1(1): 8-11 https://doi.org/10.5923/j.ijmb.20110101.03
Mekonnen, B., Cizungu, L., Alegre, J., Blondeel, H., De Lombaerde, E., Verbeeck, H., ... and De Frenne, P. (2025). Smallholder farmers knowledge on management of Cinchona in the Democratic Republic of the Congo. Journal of Plant Ecology and Evolution, 158(1), 3.
Mittal M, Gupta N, Parashar P, Mehra V. and Khatri M. Phytochemical evaluation and pharmacological activity of S. aromaticum: A comprehensive review. International Journal of Pharm Pharma Sci. 2014; 6(8): 67-72.
Oyebode Oyinlola,, Kandala Ngianga-Bakwin, Chilton Peter J and Lilford Richard J (2016): Use of traditional medicine in middle-income countries: a WHO-SAGE study. Health Policy and Planning, 2016, Vol. 31, No. 8. https://doi.org/10.1093/heapol/czw022.
Rovira-Vallbona, E.; Van Hong, N.; Kattenberg, J.H.; Huan, R.M.; Hien, N.T.T.; Ngoc, N.T.H.; Guetens, P.; Hieu, N.L.; Mai, T.T.; Duong, N.T.T.; et al. Efficacy of Dihydroartemisinin/Piperaquine and Artesunate Monotherapy for the Treatment of Uncomplicated Plasmodium falciparum Malaria in Central Vietnam. J. Antimicrob. Chemother. 2020, 75, 22722281.
Salisu, M. (2018): Ethnobotanical Assessment of Plants used for the Treatment of Malaria in Kiyawa Town, Jigawa State Nigeria. FUDMA Journal of Sciences (FJS) Vol. 2 No. 4, December, 2018, pp 148 153
Trenti, F., Yamamoto, K., Hong, B., Paetz, C., Nakamura, Y., and OConnor, S. E. (2021): Early and late steps of quinine biosynthesis. Organic Letters, 23(5), 1793-1797.
Walter K, John CC (2022). "Malaria". JAMA. 327 (6): 597. doi:10.1001/jama.2021.21468. PMID 35133414. S2CID 246651569.
World Health Organization Africa/ Countries/ Nigeria (2022): Report on malaria in Nigeria, 2022.
Yuan H, Ma Q, Ye L, Piao G. (2016): The Traditional Medicine and Modern Medicine from natural Products. Molecules, 2016; 21(5); 559.
Zongo, G., Ouattara, A., and Traore, A. (2019): HPLC-based standardization of quinine in traditional antimalarial plants. Journal of Ethnopharmacology, 12(2), 134-1
Zygmunt B. and Namiesnik, J. 2003: Preparation of Samples of Plant Material for Chromatographic Analysis. Journal of Chromatographic Science, Vol. 41, March 2003
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