EVALUATION OF THE IMPACT OF PLASMID CURING ON ANTIBIOTIC RESISTANCE ON SOME CLINICAL ISOLATES OF ESCHERICHIA COLI
Abstract
Multidrug resistance (MDR) exhibited by some strains of Escherichia coli may be due to acquiring mobile genetic element (R-plasmid) by the bacteria, or intrinsically induced by inappropriate use of antibiotics by the hosts. Infection by such strains may result to prolonged illness and greater risk of death. The study evaluated the impact of curing on antibiotic resistance on selected clinical isolates of E. coli. Twenty clinical isolates of E. coli from our previous studies were re-characterized using conventional microbiological techniques. Antibiotic sensitivity testing was determined by disk diffusion method, MDR selected based on resistance to ≥ 2 classes of antibiotics. Multiple antibiotic resistance (MAR) index was determined as ratio of the number of antibiotic resisted to the total number of antibiotics tested and considered significant if ≥. 0.2. The isolates that showed significant MAR index were subjected to plasmid curing using acridine orange, thereafter, profiled for plasmid and the cured ones were re-tested against the antibiotics they initially resisted. Out of the 20 isolates, 19 (95%) were confirmed as E. coli, all (100%) of which were MDRs, which was highest against augmentin (78.9%) followed by amoxacillin (52.6%). However, after the plasmid curing only 6 (31.6%) out of the 19 isolates cured retained significant MAR index and the level of the significance had reduced drastically in 16 (84.2%) isolates. Conclusively, curing assay can completely eliminate R-plasmid acquired resistance. More studied on plasmid curing agents for possible augmentation of the agents into antibiotics may see the rise of successful antibiotic era again.
References
Abdullahi, H.O, Maryam, A., and Machido,D.A. (2016). Isolation and Characterization of Escherichia coli O157in human stool samples from parts of Kaduna Metropolis Nigeria. American Journal of Food Science and Technology, 5:125-128.
Amer, M.M., Mekky, H.M., Amer, A.M. and Fedawy, H.S. (2018). Antimicrobial resistance
genes in pathogenic Escherichia coli isolated from diseased broiler chickens in Egypt and their relationship with the phenotypic resistance characteristics, Veterinary World, 11(8): 1082-1088
Burrssow, H.H.Canchaya, C. and Herdt, W. (2004). Phages and Evolution of the Bacteria Pathogen: from genomics rearrangement to Lysogenic conversion. Journal of Microbiology and Molecular Biology Revision, 68:560-602.
Carattoli, A. (2013). Plasmid and the spread of resistance. International Journal of medical
microbiology, (303):298-304.
Cheesebrough M. (2009). District Laboratory Practice in Tropical Countries. Cambridge University Press, 2nd ed., 96-97.
Elias, H.M., Qader, M.K. and Saleh and W.M. (2013). Determination of Plasmid DNA Role in Multidrug Resistant Pseudomonas aeruginosa Clinical Isolates. International Journal of Microbiology and Immunology, 1: 80-86.
Letchumanan, V., Chan, K. and Lee, L. (2015). An insight of Traditional Plasmid curing in Vibrio species. Frontiers in microbiology. http://doi.org/10.3389/fmicb.2015.00735
Daneman, N., Sarwar, S., Fowler, R.A., Cuthbertson, B.H. (2013). Effects of selective decontamination on antimicrobial resistance in intensive care units. Lancet Infectious Disease, 13:328-341.
DeSmet, A.M., Kluytmans, J.A., Blok, H.E., Mascini, E.M., Benus, R.F, Bernards,A.T. (2011).
Selective digestive tract decontamination and antibiotic resistance in patients in intensive care units. The Lancet,11:372-380
Duedu, K.O., Offei, G., Codjoe, F. S. and Donkor, E.S. (2017). Multidrug Resistant Enteric
Bacterial Pathogens in a Psychiatric Hospital in Ghana: Implications for Control of Nosocomial Infections. International Journal of Microbiology, https://doi.org/10.1155/2017/9509087
Giwa, F.J., Ige, O.T., Haruna, D.M., Yaqub, Y., Lamido, T.Z. and Usman, S.Y. (2018).
Extended-Spectrum beta-lactamase production and antimicrobial susceptibility pattern of uropathogens in a Tertiary Hospital in Northwestern Nigeria. Annals of Tropical Pathology, 9:11-6.
Guillermo, V.S., Ronald, N.M. and Jose, M.B. (2012). In vitro antimicrobial resistance of urinary Escherichia coli isolates among U.S outpatients from 2000-2010. Antimicrobial Agents and Chemotjerapy,56(4):2181-2183
Kasumi, I., Tazumi, S., Furuya, N., Komano, T. (2013). Exclusion A proteins of Inc1 plasmid R64 and Inc1y plasmid R621a recognize different segments of their cognate Tra Y proteins in entry exclusion plasmid, Springer, 69:138-145
Olukoya, D.K. and Oni, O. (1990). Plasmid Profile Analysis and Antimicrobial Susceptibility Patterns of shigella isolates from Nigeria. Epidemiology and Infection,105:59-64
Oriomah, C., Akpe, A.R. (2019). Plasmid curing of antibiotic resistant Escherichia coli isolates from urine and stool samples. Journal of Microbiology and Antimicrobials,11:1-4.
Rogers, B.A., Kennedy, K.J., Sidjabat, H.E., Jones, M., Collignon, P. and Paterson, D.I. (2012). Prolonged, carriage of resistant E. coli by returned travelers. European Journal of Clinical Investigation,13:328-341
Sulaiman, M.A. and Usman, A. R. (2020). Assessment of Potential Efficacy of Coliphage
Therapy on Multidrug Resistant Clinical Isolates of Escherichia coli. UMYU Journal of Microbiology Research,5 (1)49-53.
Sulaiman, M.A., Aminu, M., Ella, E.E. and Abdullahi, I.O. (2020). Characterization of
Escherichia albertii, Shigella species and Diarrhoeagenic Escherichia coli from stool samples of gastroenteritis patients in Nano state, Nigeria. Ph.D Thesis, Department of Microbiology, Ahmadu Bello university, Zaria.
Wayne, P.A (2018). CLSI Performance Standards for Antimicrobial Susceptibility Testing.28th ed. CLSI supplement M100. Clinical and Laboratory Standards Institute
Zaman, M.A, Pasha, M.H. and Akhter, M.Z. (2010). Plasmid curing of Escherichia coli cells with ethidium bromide, sodium dodecyl sulfate and acridine orange. Bangladesh Journal of Microbiology,27(1):28-31.
Zhang, R., Pan, L., and Zhao, Z.(2012). High Incidence of plasmid in marine Vibrio species isolated from Mai po Nature Reserve of Hong Kong. Ecotoxicology, 21 (6):1661-1668
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