ACUTE TOXICITY TEST AND EVALUATION OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF METHANOL LEAF EXTRACT OF OCHNA KIBBIENSIS HUTCH AND DALZ (OCHNACEAE)

  • Muhammad Abubakar Alhaji Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University, Sokoto
  • Abdullahi Musa Ismaila Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University, Sokoto
  • Amina Jega Yusuf Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University, Sokoto
  • Yahaya Muhammad Sani Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Zaria
  • Nasir Ibrahim Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University, Sokoto
  • Muhammad Koko Buhari Department of Chemistry, Federal University Gusau
  • Abdullahi Isah Department of Science Laboratory Technology, Umaru Ali Shinkafi Polytechnic, Sokoto
Keywords: Ochna kibbiensis, Toxicity, Antinociceptive, Analgesic, Anti-inflammatory

Abstract

Pain is an unpleasant feeling caused by the stimulation of pain receptors. Inflammation is a complex response to exogenous and endogenous stimuli. Ochna kibbiensis Hutz and Dalz (Ochnaceae) is majorly used traditionally in the treatment of pain, inflammation and malaria. This study aimed to evaluate the analgesic and anti-inflammatory properties of the leaf extract of the plant. The analgesic activity of the Methanol leaf extract (MLE) was evaluated using acetic acid-induced writhing in mice and formalin-induced pain in rat models while the anti-inflammatory studies were carried out using formalin-induced inflammation in rats. In the acetic acid model, the MLE in graded doses (40, 80 and 160 mg/kg) significantly (p0.05) and in a concentration-dependent manner reduced the number of writhes induced by acetic acid. Similarly, the extract significantly (p0.05) reduced pain induced by formalin in both phases, with the highest dose (160 mg/kg) having a higher % inhibition than the standard drug, Piroxicam (10 mg/kg). In the formalin-induced inflammation test in rats, at the fourth hour, all the doses significantly (p0.05) in a concentration-dependent manner reduced the paw oedema of the rat. To the best of our knowledge, this is the first report of the evaluation of the analgesic and anti-inflammatory activities of the methanol leaf extract of O. kibbiensis.

References

Abdullahi, M. I., Haruna, A.K., Musa, M.A., Alhassan, M.A., Umar, A., Yusuf, A.J., Uba, A. and Ibrahim, Z.Y.Y. (2015). Preliminary phytochemical and antimicrobial evaluation of the methanolic leaf extract of Ochna Kibbiensis Hutz and Dalz (Ochnaceae). Journal of Chemical and Pharmaceutical Research. 7(2): 626-631. https://www.researchgate.net/publication/275888847

Abdullahi, M. I., Musa, A. M., Nasir, T., Magaji, M. G., Yusuf, A. J. and Iliya, I. (2016). Anti-proliferative study and isolation of ochnaflavone from the ethyl acetate-soluble fraction of Ochna kibbiensis Hutch and Dalziel. Natural Product Research, 31(18): 2149-2152. http://doi.org/10.1080/14786419.2016.1274892. DOI: https://doi.org/10.1080/14786419.2016.1274892

Abdullahi, I. D., Gudaji, M. I. And Yaro, A. H. (2024). Modulation of cytokine expression and antimalarial activity of residual aqueous fraction of the methanol leaf extract of Ficus asperifolia in Plasmodium berghei-infected mice. FUDMA Journal of Sciences, 8(2): https://doi.org/10.33003/fjs-2024-0802-2160 DOI: https://doi.org/10.33003/fjs-2024-0802-2160

Barcellos, J. D., Grossman, E., Navas, D. M., Junior, J. O., Fonseca, P. R. B and Posso, I. P. (2017). Prevalence of Chronic Pain, Treatments, Perception, and Interference on Life Activities: Brazilian Population-Based Survey. Pain Research and Management, 2017: 1-9. https://doi.org/10.1155/2017/4643830. DOI: https://doi.org/10.1155/2017/4643830

Bature, H. B., Tsafe, A. I., Zauro, S. A. and Yusuf, A. J. (2022). EFFECT OF NEOCARYA MACROPHYLLA LEAVES EXTRACTS ON MURINE MODEL OF PAIN. FUDMA Journal of Sciences, 6(5): 264-269 https://doi.org/10.33003/fjs-2022-0605-1186 DOI: https://doi.org/10.33003/fjs-2022-0605-1752

Beivit, H., Borchgrevink, P. C., Allen, S. M., Rosseland, L. A., Romundstad, L., Breivik Hals, E. K., Krarstein, G. and Stubhaug A. (2008). Assessment of pain. British Journal of Anaesthesia, 101(1): 17-24. https://doi.org/10.1093/bja/aen103. DOI: https://doi.org/10.1093/bja/aen103

Burkill, H.M. (1985). The useful plants of west tropical Africa, Royal Botanic Gardens, Kew. Richmond, United Kingdom, 4: 445.

Bushra, B.N.R. and Ganga, D.T. (2003). Antibacterial activity of selected seaweeds from Kovalam south west of India. Asian journal of microbiology, Biotechnology and Environmental sciences, 5(3):319-322. https://www.researchgate.net/publication/286605101.

Christenhust, M. J. M. and Byng, J. W (2016). The number of known plants species in the world and its annual increase. Phytotaxa. 261(3): 201-217. https://doi.org/10.11646/phytotaxa.261.3.1. DOI: https://doi.org/10.11646/phytotaxa.261.3.1

Coelho, L. P., Reis, P. A., de Castro, F. L., Gayer, C. R. M., da Silva Lopes, C., da Costa e Silva, M. C., de Carvalho Sabino, K. C., Todeschini, A. R. and Coelho, M. G. (2005). Antinociceptive Properties of Ethanol Extract and Fractions of Perodon pubescence Benth seeds. Journal of Ethnopharmacology. 98 (1-2): 109-116. https://doi.org/10.1016/j.jep.2005.01.014. DOI: https://doi.org/10.1016/j.jep.2005.01.014

Debono, D. J., Hoeksema, L. J. and Hobbs, R. D (2013). Caring for Patients with Chronic Pain: Pearls and Pitfalls. Journal of the American Osteopathic Association. 113(8): 620-627 https://doi.org/10.7556/jaoa.2013.023. DOI: https://doi.org/10.7556/jaoa.2013.023

Deradt, R., Jongney, S., Deivaicee, F. and Falhout, M. (1980). Release of prostaglandins E and F in an algogenic reaction and its inhibition. European Journal of Pharmacology. 61(1): 17-24. https://doi.org/10.1016/0014-2999(80)90377-5. DOI: https://doi.org/10.1016/0014-2999(80)90377-5

Dhara, A. K., Suba, V., Sen, T., Pal, S. and Chaudhuri, A. K. (2000). Preliminary studies on the anti-inflammatory and analgesic activity of methanol fraction of the root of Tragia involucrate. Journal of Ethnopharmacology. 72(1-2): 265-268. https://doi.org/10.1016/s0378-8741(00)00166-5. DOI: https://doi.org/10.1016/S0378-8741(00)00166-5

Djova, S. V., Nyegue, M. A., Messi, A. N., Afagnigni, A. D. and Etoa, F. X. (2019). Phytochemical Study of Aqueous Extract of Ochna schweinfurthiana F. Hoffm Powder Bark and Evaluation of Their Anti-Inflammatory, Cytotoxic, and Genotoxic Properties. Evidence-Based Complementary and Alternative Medicine, https://doi.org/10.1155/2019/8908343. DOI: https://doi.org/10.1155/2019/8908343

Dubuisson, D. and Dennis, S. G. (1977). The formalin test: quantitative studies of the analgesic effect of morphine, meperidine and brain stem stimulation in rats and cats. Pain, 4(2):161-174. https://doi.org/10.1016/0304-3959(77)90130-0. DOI: https://doi.org/10.1016/0304-3959(77)90130-0

Fantone, J. C. and Ward, P. A. (1982). Role of Oxygen-derived Free Radicals and Metabolites in leukocyte-dependant Inflammatory reactions. American Journal of Pathology. 107(3): 395-418.

Ibrahim, Z. Y. Y., Musa, A. M., Abdullahi, M. I., Uba, A., Yusuf, A. J., Magaji, M. J.Aliyu, I. M and Yau, J. (2015). Phytochemical and antinociceptive studies on the methanol leaf extract of Ochna schweinfurthiana (ochnaceae). International Journal of Advances in Pharmacy, Biology and Chemistry. 4(4): 838-843.

Imam, M. Z., Nahar, N., Akter, S. and Rana, M. S. (2012). Antinociceptive Activity of Methanol Extract of Flowers of Impatient balsamina. Journal of Ethnopharmacology. 142(3): 802-810. https://doi.org/10.1016/j.jep.2012.06.004. DOI: https://doi.org/10.1016/j.jep.2012.06.004

Khan, H., saeed, M., Gilani, A. U. H., Khan, M. A., Dar, A. and Khan I. (2010). The antinociceptive activity of Polygonatum verticillatum rhizomes in pain models. Journal of Ethnopharmacology, 127(2): 521-527. https://doi.org/10.1016/j.jep.2009.10.003. DOI: https://doi.org/10.1016/j.jep.2009.10.003

Kumar, V. R. And Srinivas, L. (2022). In vitro anti-cancer, anti-inflammatory and anti-arthritic activity of ethanolic extract of Ochna obtusata leaves. Research Journal of Pharmacy and Technology, 15(9): 3999-4004. http://dx.doi.org/10.52711/0974-360X.2022.00670 DOI: https://doi.org/10.52711/0974-360X.2022.00670

Liu, N., Zhang, G., Niuu, Y., Wang, Q., Zheng, J., Yang, J., Sun, T., Niu, J. and Yu, J. (2020). Anti-inflammatory and analgesic activities of indigo through regulating the IKK/IB/NF-B pathway in mice. Food and Function, 2020(11): 8537-8546. https://doi.org/10.1039/C9FO02574J DOI: https://doi.org/10.1039/C9FO02574J

Liu, Y., Yang, C., Zhang, J., Ihsan, A., Ares, I., Martinez, M., Lopez-Torres, B., Martinez-Larranaga, M., Wang, X., Anadon, A. and Martinez, M. (2024).Recent progress in adverse events of carboxylic acid non-steroidal anti-inflammatory drugs (CBA-NSAIDs) and their association with the metabolism: the consequences on mitochondrial dysfunction and oxidative stress, and prevention with natural plant extracts. Expert Opinion on Drug Metabolism and Toxicology, 20(8): https://doi.org/10.1080/17425255.2024.2378885 DOI: https://doi.org/10.1080/17425255.2024.2378885

Lorke, D. (1983). A new Approach to Practical Acute Toxicity Testing. Archives of Toxicology, 54(4): 275-287. https://doi.org/10.1007/bf01234480. DOI: https://doi.org/10.1007/BF01234480

Magadum, S. B., Khandale, M. and Limaye, R. P. (2015). The study of anti-inflammatory activity of Buspirone in rats. International Journal of Healthcare and Biomedical Research. 04(01): 51-59.

Orlando FA and Mainous AG III (2024). Editorial: Inflammation and chronic disease. Frontier in Medicine. 11:1434533. https://doi.org/10.3389/fmed.2024.1434533 DOI: https://doi.org/10.3389/fmed.2024.1434533

Oronsky, B.; Caroen, S.; Reid, T. (2022). What Exactly Is Inflammation (and What Is It Not?). International Journal Molecular Science 23, 14905. https://doi.org/10.3390/ijms232314905 DOI: https://doi.org/10.3390/ijms232314905

Tjlsen A., Berge, O., Hunskaar, S., Rosland, J. H. and Hole, K. (1992). The formalin test: an evaluation of the method. The Journal of International Association for the study of Pain, 51(1): 5-17. https://doi.org/10.1016/0304-3959(92)90003-T DOI: https://doi.org/10.1016/0304-3959(92)90003-T

Shibata, M., Ohnkubo, T., Takahashi, H. and Inoki, R. (1989). Modified formalin test: characteristic biphasic pain response. Pain, 38(3): 347-352. https://doi.org/10.1016/0304-3959(89)90222-4 DOI: https://doi.org/10.1016/0304-3959(89)90222-4

Shumaia, P., Abu Shuaib, R., Abdul, K., Most Afia, A. and Tahmida, S. (2014). Phytochemical screening and studies of analgesic potential of Moring oleifera Lam. stem bark extract on experimental animal model. International Journal of Phytopharmacy. 4(5): 128-131. http://dx.doi.org/10.7439/ijpp.v4i5.107.

Suh, S. J., Jin, U. H. and Kim, S. H. (2006). Ochnaflavone inhibits TNF--induced human VSMC proliferation via regulation of cell cycle, ERK1/2 and MMP-9. Journal of Cellular Biochemistry, 99(5):1298-1307. https://doi.org/10.1002/jcb.20912 DOI: https://doi.org/10.1002/jcb.20912

Zhang Y, Song M, Yang Z, Huang X, Lin Y, Yang H. Healthy lifestyles, systemic inflammation, and breast cancer risk: a mediation analysis. BMC Cancer. (2024) 24:208. https://doi.org/10.1186/s12885-024-11931-5 DOI: https://doi.org/10.1186/s12885-024-11931-5

Zheng J, Liu M, Zhao L, Hbert JR, Steck SE, Wang H, et al. Dietary inflammatory potential, inflammation-related lifestyle factors, and incident anxiety disorders: a prospective cohort study. Nutrients. (2023) 16:121. https://doi.org/10.3390/nu16010121 DOI: https://doi.org/10.3390/nu16010121

Published
2025-06-30
How to Cite
Alhaji, M. A., Ismaila, A. M., Yusuf, A. J., Sani, Y. M., Ibrahim, N., Buhari, M. K., & Isah, A. (2025). ACUTE TOXICITY TEST AND EVALUATION OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF METHANOL LEAF EXTRACT OF OCHNA KIBBIENSIS HUTCH AND DALZ (OCHNACEAE). FUDMA JOURNAL OF SCIENCES, 9(6), 105 - 109. https://doi.org/10.33003/fjs-2025-0906-3224