OPTIMIZATION AND CHARACTERIZATION OF PECTINASE PRODUCED BY ASPERGILLUS FLAVUS SS13 USING CITRUS PEEL

Authors

  • Mohammed I. Ja'afaru
    Modibbo Adama University, Yola, Faculty of Life Sciences, Department of Microbiology
  • Justice O. Ogwuche
    Modibbo Adama University, Yola, Faculty of Life Sciences, Department of Microbiology
  • Olumuyiwa M. Adeyemo
    Department of Biotechnology, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa State
  • Adati L. Bernard
    Modibbo Adama University, Yola, Faculty of Life Sciences, Department of Microbiology

Keywords:

Aspergillus flavus SS13, Pectinases, Mutagenesis, Optimization, Characterization

Abstract

Pectinases have multipurpose applications in both industrial and biotechnological sectors. But they are expensive when available. This study was carried out to isolate fungi, screen them for pectinase activity, identify selected fungi isolate, mutate, optimize, produce, and characterize the partially purified pectinase. Aspergillus flavus SS13 was isolated using standard technique, it exhibited the highest pectinase activity of 36.0±0.3 mm zone of hydrolysis and it was identified based on its macroscopic and microscopic characteristics. EMS mutated A. flavus SS13 had a pectinase activity of 40±0.2 U/mL, UV-mutated A. flavus SS13 exhibited 36.0±0.5 U/mL while the SA-mutated A. flavus SS13 showed pectinase activitiy of 35±0.4 U/mL, and unmutated A flavus SS13 had 28.0 ±0.2 U/mL at pH 3.0, temperature 40 °C and incubation period of 96 h utilizing orange peel as carbon. The partially purified pectinase of A. flavus SS13, A. flavus SS13-EMS, A. flavus SS13-UV, and A. flavus SS13-SA had between 1.3 to 1.5 fold purification and 26.0 to 30.0 % recovery. The partially purified pectinase of A. flavus SS13 wild type and the three mutants had improved activity in the presence of tween-80, while a significant pectinase activity was noted with cations such as Cu2+, Ca2+, and K+ in all the three mutants’ enzyme while the ions of ammonium, cobalt, and sodium repressed pectinase activity in the wild type A. flavus SS13. The stability of the pectinase produced by the mutants at acidic pH and elevated temperature could be an added advantage for its diverse applications in the food, detergent,...

Author Biographies

Mohammed I. Ja'afaru

 Professor Department of Microbiology

Justice O. Ogwuche

 Graduate Assistant, Department of Microbiology 

Dimensions

Adedayo, M., Mohammed, M., Ajiboye, A., & Abdulmumini, S. (2021). Pectinolytic activity of Aspergillus niger and Aspergillus flavus grown on grapefruit (citrus Parasidis) peel in solid state fermentation. Global Journal of Pure and Applied Sciences, 27(2), 93-105.

Adeleke, A. J., Odunfa, S. A., Olanbiwonninu, A., & Owoseni, M. C. (2012). Production of cellulase and pectinase from orange peels by fungi. Nature and Science, 10(5), 107-112.

Ahmed, I., Zia, M., Azhar, M., Akram, Z., Naveed, T., & Nowrouzi, A. (2015). Bioprocessing of citrus waste peel for induced pectinase production by Aspergillus niger; its purification and characterization. Journal of Radiation Research and Applied Sciences, 9. https://doi.org/10.1016/j.jrras.2015.11.003

Ajayi, A., Lawal, B., Salubi, A., Onibokun, A., Oniha, M., & Ajayi, O. (2021). Pectinase production by Aspergillus niger using pineapple peel pectin and its application in coconut oil extraction. Paper presented at the IOP Conference Series: Earth and Environmental Science.

Akbar, S., Prasuna, R., & Khanam, R. (2015). Strain improvement by induction of mutagenesis for hyperproduction of pectinase using Aspergillus tamarii. Journal of Scientific and Industrial Research, 74(3), 160-164

Alqahtani, Y. S., More, S. S., R, K., & Shaikh, I. A. (2022). Production and Purification of Pectinase from Bacillus subtilis 15A-B92 and Its Biotechnological Applications. 27(13). https://doi.org/10.3390/molecules27134195

Amilia, K., Sari, S., & Setyaningsih, R. (2017). Isolation and screening of pectinolytic fungi from orange (Citrus nobilis Tan.) and banana (Musa acuminata L.) fruit peel. Paper presented at the IOP Conference Series: Materials Science and Engineering. 2017: IOP Publishing.

Antier, P., Minjares, A., Roussos, S., Raimbault, M., & Viniegra-Gonzalez, G. (1993). Pectinase-hyperproducing mutants of Aspergillus niger C28B25 for solid-state fermentation of coffee pulp. Enzyme and Microbial Technology, 15(3), 254-260. https://doi.org/10.1016/0141-0229(93)90146-s

Banu, A., Devi, M., Gnanaprabhal, G. R., B.V, P., & Muthusamy, P. (2010). Production and characterization of pectinase enzyme from Penicillium chrysogenum. Indian Journal of Science and Technology, 3, 377-381. https://doi.org/10.17485/ijst/2010/v3i4/29721

Carmona, E. C., Brochetto-braga, M. R., Pizzirani-kleiner, A. A., & Jorge, J. A. (1998). Purification and biochemical characterization of an endoxylanase from Aspergillus versicolor. FEMS Microbiology Letters, 166(2), 311-315. doi: https://doi.org/10.1016/S0378-1097(98)00348-6

Carrasco, M., Rozas, J. M., Alcano, J., Cifuentes, V., & Baeza, M. (2019). Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp. Microbial Cell Factories, 18, 1-11.

Domsch, K. H., Gams, W., & Anderson, T.-H. (1980). Compendium of soil fungi. Volume 1: Academic Press (London) Ltd.

Ezugwu, A. L., Onoyima, S. C., Eje, O. E., Nsude, C. A., Ugoh, A. I., & Chilaka, F. C. (2023). Properties and application of pectinase obtained from Galactomyces candidum using pectin extracted from mango peels as a carbon source. Biocatalysis and Agricultural Biotechnology, 51, 102763. doi: https://doi.org/10.1016/j.bcab.2023.102763

G, N., Taze, B. H., Demir, H., Tari, C., nltrk, S., & Lahore, M. F. (2014). Evaluation of orange peel, an industrial waste, for the production of Aspergillus sojae polygalacturonase considering both morphology and rheology effects. Turkish Journal of Biology, 38(4), 537-548.

Haile, S., & Ayele, A. (2022). Pectinase from Microorganisms and Its Industrial Applications. 2022, 1881305. doi: 10.1155/2022/1881305

Jekayinfa, S. O., Orisaleye, J. I., & Pecenka, R. (2020). An assessment of potential resources for biomass energy in Nigeria. Resources, 9(8), 92. https://doi.org/10.3390/resources9080092

Kadija Tul Kubra, S. A., & Manam Walait, H. S. Potential Applications of Pectinases in Food, Agricultural, and Environmental Sectors. Journal of Pharmaceutical, Chemical, and Biological Sciences, 6(2), 25-34.

Kamalambigeswari, R., Alagar, S., & Sivvaswamy, N. (2018). Strain improvement through mutation to enhance pectinase yield from Aspergillus niger and molecular characterization of polygalactouronase gene. Journal of Pharmaceutical Sciences and Research, 10, 989-994.

Kaur, A., Singh, A., Dua, A., & Mahajan, R. (2016). Cost-effective and concurrent production of industrially valuable xylano-pectinolytic enzymes by a bacterial isolate Bacillus pumilus AJK. Preparative Biochemistry & Biotechnology, 47. https://doi.org/10.1080/10826068.2016.1155059

KC, S., Upadhyaya, J., Joshi, D. R., Lekhak, B., Kumar Chaudhary, D., Raj Pant, B., . . . Koirala, N. (2020). Production, characterization, and industrial application of pectinase enzyme isolated from fungal strains. Fermentation, 6(2), 59.

Ketipally, R., & Ram, M. R. (2018). Optimization of pectinase production by Aspergillus oryzae RR 103. Current Agriculture Research Journal, 6(1), 37.

Khatri, B. P., Bhattarai, T., Shrestha, S., & Maharjan, J. (2015). Alkaline thermostable pectinase enzyme from Aspergillus niger strain MCAS2 isolated from Manaslu Conservation Area, Gorkha, Nepal. SpringerPlus, 4(1), 1-8.

Madika, A., Eugene, U. A., Bishir, M., Sulaiman, M. A., & Hussaini, I. M. (2020). Screening of Aspergillus niger isolated from soil for pectinase production. FUDMA Journal of Sciences, 4(2), 244-249.

Miller, G. L. (1959). Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry, 31(3), 426-428. https://doi.org/10.1021/ac60147a030

Okafor, U., Okochi, I., Chinedu, S. N., Ebuehi, O., & Onyegeme-Okerenta, B. (2010). Pectinolytic activity of wild-type filamentous fungi fermented on agro-wastes. African Journal of Microbiology Research, 4, 2729-2734.

Okonji, R. E., Itakorode, B. O., Ovumedia, J. O., & Adedeji, O. S. (2019a). Purification and biochemical characterization of pectinase produced by Aspergillus fumigatus isolated from soil of decomposing plant materials. Journal of Applied Biology and Biotechnology, 7(3), 1-8.

Patil, S., & Dayanand, A. (2006). Production of pectinase from deseeded sunflower head by Aspergillus niger in submerged and solid-state conditions. Bioresource Technology, 97, 2054-2058. doi: 10.1016/j.biortech.2005.09.015

Phutela, U., Dhuna, V., Sandhu, S., & Chadha, B. S. (2005). Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposing orange peels. Brazilian Journal of Microbiology, 36. 63-69. https://doi.org/10.1590/S1517-83822005000100013

Popa, A., Israel-Roming, F., & Cornea, C. P. (2017). Fungal strain improvement for xylanase overproduction through physical and chemical mutagenesis. 6. Research Journal of Agricultural Sciences, 48(2), 2016.

Premjet, S. (2012). Enhanced cellulose production by ultraviolet (UV) irradiation and N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis of an Acetobacter species isolates. African Journal of Biotechnology, 11. https://doi.org/10.5897/AJB11.3158

Shin, I., Dowmez, S., & Kilic, O. (1983). Study on pectolytic enzyme production from some agricultural wastes by fungi. Chemie Microbiologie Technologie der Lebensm, 8, 87-90.

Singh, S., Pathak, S., Sandhu, S., & Rajak, R. (2015). Mutation studies on fungal glucoamylase: A review. International Journal of Pharmacy and Biological Sciences, 5, 297-308.

Soares, M., Da Silva, R., & Gomes, E. (1999). Screening of bacterial strains for pectinolytic activity: Characterization of the polygalacturonase produced by Bacillus sp. Revista de Microbiologia, 30. https://doi.org/10.1590/S0001-37141999000400002

Tari, C., Dogan, N., & Gogus, N. (2008). Biochemical and thermal characterization of crude exo-polygalacturonase produced by Aspergillus sojae. Food Chemistry, 111(4), 824-829.

Thakur, A., Pahwa, R., Singh, S., & Gupta, R. (2010). Production, purification, and characterization of polygalacturonase from Mucor circinelloides ITCC 6025. Enzyme Research, 2010 (2), 170549. http://dx.doi.org/10.4061/2010/170549

Zhang, G., Li, S., Xu, Y., Wang, J., Wang, F., Xin, Y., Liu, H. (2019). Production of alkaline pectinase: a case study investigating the use of tobacco stalk with the newly isolated strain Bacillus tequilensis CAS-MEI-2-33. BMC Biotechnology. 19(1), 45. https://doi.org/10.1186/s12896-019-0526-6

Published

31-01-2025

How to Cite

OPTIMIZATION AND CHARACTERIZATION OF PECTINASE PRODUCED BY ASPERGILLUS FLAVUS SS13 USING CITRUS PEEL. (2025). FUDMA JOURNAL OF SCIENCES, 9(1), 186-195. https://doi.org/10.33003/fjs-2025-0901-3014

Issue

Vol. 9 No. 1 (2025): FUDMA Journal of Sciences - Vol. 9 No. 1

Section

Research Articles
Copyright & Licensing

FUDMA Journal of Sciences

How to Cite

OPTIMIZATION AND CHARACTERIZATION OF PECTINASE PRODUCED BY ASPERGILLUS FLAVUS SS13 USING CITRUS PEEL. (2025). FUDMA JOURNAL OF SCIENCES, 9(1), 186-195. https://doi.org/10.33003/fjs-2025-0901-3014